LOCUS       sequence-204855-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Functional studies using RNAi; rescue experiments; substituting 
            protein for its different form. miRNA cassette with nuclear EGFP 
            expression marker; your protein of interest with C-terminal FLAG..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Szczesny RJ, Kowalska K, Klosowska-Kosicka K, Chlebowski A, Owczarek
            EP, Warkocki Z, Kulinski TM, Adamska D, Affek K, Jedroszkowiak A, 
            Kotrys AV, Tomecki R, Krawczyk PS, Borowski LS, Dziembowski A
  TITLE     Versatile approach for functional analysis of human proteins and 
            efficient stable cell line generation using FLP-mediated 
            recombination system.
  JOURNAL   PLoS One. 2018 Mar 28;13(3):e0194887. doi: 
            10.1371/journal.pone.0194887. eCollection 2018.
  PUBMED    29590189
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-03-28"; volume: "13(3):e0194887. doi"; pages: " 
            10.1371/journal.pone.0194887. eCollection 2018"
COMMENT     Sequence Label: pKK-RNAi-nucEGFPmiR-TEV-FLAG
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(41..82)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(146..172)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(179..220)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(284..310)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(317..358)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(422..448)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     CDS             complement(494..514)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(518..538)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(542..562)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(617..638)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctctagatca ctagatatga cgcgtgcggc cgcagatctg ggccatttgt tccatgtgag
       61 tgctagtaac aggccttgtg tcctgaaatg tactgcgtgg agacgtcagt cagtggccaa
      121 aacgtctcca cgcgcagtac atttcagcat acagccttca gcaagcctcc aggatctggg
      181 ccatttgttc catgtgagtg ctagtaacag gccttgtgtc ctgaaatgta ctgcgtggag
      241 acgtcagtca gtggccaaaa cgtctccacg cgcagtacat ttcagcatac agccttcagc
      301 aagcctccag gatctgggcc atttgttcca tgtgagtgct agtaacaggc cttgtgtcct
      361 gaaatgtact gcgtggagac gtcagtcagt ggccaaaacg tctccacgcg cagtacattt
      421 cagcatacag ccttcagcaa gcctccagga tcacttaaga ctagcgctcc aattgcacta
      481 cctcctctta ctacaccttg cgtttcttct ttgggtcgac ctttctcttc tttttagggt
      541 ccaccttccg ctttttcttg gggtctgctg cggccttgta cagctcgtcc atgccgagag
      601 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      661 ctttgctc
//
