LOCUS       sequence-20486-e         605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GAL-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(116..240)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(317..338)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(578..597)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
       61 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag
      121 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
      181 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
      241 gatgggctgc aggaattcga tatcaagctt aatggtgagc aagggcgagg agctgttcac
      301 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      361 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      421 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      481 gtgcttcagc cgctaccccg accacatgaa gcancacgac ttcttcaagt ccgccatgcc
      541 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      601 cgccg
//
