LOCUS       sequence-204862-        9311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Chinthalapudi K, Rangarajan ES, Brown DT, Izard T
  TITLE     Differential lipid binding of vinculin isoforms promotes 
            quasi-equivalent dimerization.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):9539-44. doi: 
            10.1073/pnas.1600702113. Epub 2016 Aug 8.
  PUBMED    27503891
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1600702113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-08-23- 23"; volume: "113"; issue: "34"; pages: "9539-44"
COMMENT     Sequence Label: pcDNA4-GFP-Metavinculin
FEATURES             Location/Qualifiers
     source          1..9311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        37..416
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        417..620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     571..591
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    622..640
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    643..661
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     671..695
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             807..836
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(837..839,840..842,843..1553)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(837..839,840..842,843..1553)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(882..903)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1143..1162)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1490..1511
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1560..1583
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5036..5065
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5081..5098
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5121..5138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5127..5351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5397..5825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5484..5503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5694..5715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5834..5854)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5839..6168
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6019..6154
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6081..6100
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6216..6263
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6282..6656
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAGWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6786..6907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6823..6842)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6877..6896
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6956..6972)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6956..6972)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6969..6991)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6980..6996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7004..7010,7011..7028,7029..7034))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7049..7070
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7187..7204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7358..7946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7438..7457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8117..8908,8909..8977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8740..8759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8978..9082)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9157..9176)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
       61 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      121 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      181 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      241 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      301 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      361 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      421 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      481 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      541 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      601 aggtctatat aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
      661 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      721 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
      781 agcttgctag cggtaccgcc accatgcatc accaccacca tcatcatcac caccatatgg
      841 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      901 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      961 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1021 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1081 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1141 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1201 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1261 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1321 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1381 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1441 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1501 tggagttcgt gaccgccgcc gggatcaccc tcggcatgga cgagctgtac aagggatccc
     1561 tggaagttct gttccagggg cccgccgccg ctatgccagt gtttcatacg cgcacgatcg
     1621 agagcatcct ggagccggtg gcacagcaga tctcccacct ggtgataatg cacgaggagg
     1681 gcgaggtgga cggcaaagcc attcctgacc tcaccgcgcc cgtggccgcc gtgcaggcgg
     1741 ccgtcagcaa cctcgtccgg gttggaaaag agactgttca aaccactgag gatcagattt
     1801 tgaagagaga tatgccacca gcatttatta aggttgagaa tgcttgcacc aagcttgtcc
     1861 aggcagctca gatgcttcag tcagaccctt actcagtgcc tgctcgagat tatctaattg
     1921 atgggtcaag gggcatcctc tctggaacat cagacctgct ccttaccttc gatgaggctg
     1981 aggtccgtaa aattattaga gtttgcaaag gaattttgga atatcttaca gtggcagagg
     2041 tggtggagac tatggaagat ttggtcactt acacaaagaa tcttgggcca ggaatgacta
     2101 agatggccaa gatgattgac gagagacagc aggagctcac tcaccaggag caccgagtga
     2161 tgttggtgaa ctcgatgaac accgtgaaag agttgctgcc agttctcatt tcagctatga
     2221 agatttttgt aacaactaaa aactcaaaaa accaaggcat agaggaagct ttaaaaaatc
     2281 gcaattttac tgtagaaaaa atgagtgctg aaattaatga gataattcgt gtgttacaac
     2341 tcacctcttg ggatgaagat gcctgggcca gcaaggacac tgaagccatg aagagagcat
     2401 tggcctccat agactccaaa ctgaaccagg ccaaaggttg gctccgtgac cctagtgcct
     2461 ccccagggga tgctggtgag caggccatca gacagatctt agatgaagct ggaaaagttg
     2521 gtgaactctg tgcaggcaaa gaacgcaggg agattctggg aacttgcaaa atgctagggc
     2581 agatgactga tcaagtggct gacctccgtg ccagaggaca aggatcctca ccggtggcca
     2641 tgcagaaagc tcagcaggta tctcagggtc tggatgtgct cacagcaaaa gtggaaaatg
     2701 cagctcgcaa gctggaagcc atgaccaact caaagcagag cattgcaaag aagatcgatg
     2761 ctgctcagaa ctggcttgca gatccaaatg gtggaccgga aggagaagag cagattcgag
     2821 gtgctttggc tgaagctcgg aaaatagcag aattatgtga tgatcctaaa gaaagagatg
     2881 acattctacg ttcccttggg gaaatatctg ctctgacttc taaattagca gatctacgaa
     2941 gacaggggaa aggagattct ccagaggctc gagccttggc caaacaggtg gccacggccc
     3001 tgcagaacct gcagaccaaa accaaccggg ctgtggccaa cagcagaccg gccaaagcag
     3061 ctgtacacct tgagggcaag attgagcaag cacagcggtg gattgataat cccacagtgg
     3121 atgaccgtgg agtcggtcag gctgccatcc gggggcttgt ggccgaaggg catcgtctgg
     3181 ctaatgttat gatggggcct tatcggcaag atcttctcgc caagtgtgac cgagtggacc
     3241 agctgacagc ccagctggct gacctggctg ccagagggga aggggagagt cctcaggcac
     3301 gagcacttgc atctcagctc caagactcct taaaggatct aaaagctcgg atgcaggagg
     3361 ccatgactca ggaagtgtca gatgttttca gcgataccac aactcccatc aagctgttgg
     3421 cagtggcagc cacggcgcct cctgatgcgc ctaacaggga agaggtattt gatgagaggg
     3481 cagctaactt tgaaaaccat tcaggaaagc ttggtgctac ggccgagaag gcggctgcgg
     3541 ttggtactgc taataaatca acagtggaag gcattcaggc ctcagtgaag acggcccgag
     3601 aactcacacc ccaggtggtc tcggctgctc gtatcttact taggaaccct ggaaatcaag
     3661 ctgcttatga acattttgag accatgaaga accagtggat cgataatgtt gaaaaaatga
     3721 cagggctggt ggacgaagcc attgatacca aatctctgtt ggatgcttca gaagaagcaa
     3781 ttaaaaaaga cctggacaag tgcaaggtag ctatggccaa cattcagcct cagatgctgg
     3841 ttgctggggc aaccagtatt gctcgtcggg ccaaccggat cctgctggtg gctaagaggg
     3901 aggtggagaa ttccgaggat cccaagttcc gtgaggctgt gaaagctgcc tctgatgaat
     3961 tgagcaaaac catctcccca atggtgatgg atgcaaaagc tgtggctgga aacatttccg
     4021 accctggact gcaaaagagc ttcctggact caggatatcg gatcctggga gctgtggcca
     4081 aggtcagaga agccttccaa cctcaggagc ctgacttccc gccgcctcca ccagaccttg
     4141 aacaactccg actaacagat gagcttgctc ctcccaaacc acctctgcct gaaggtgagg
     4201 tccctccacc taggcctcca ccaccagagg aaaaggatga agagttccct gagcagaagg
     4261 ccggggaggt gattaaccag ccaatgatga tggctgccag acagctccat gatgaagctc
     4321 gcaaatggtc cagcaagccg ggcatcccag ccgctgaggt gggtataggt gttgtagctg
     4381 aggcagatgc ggccgatgct gctggcttcc ctgtcccccc tgacatggaa gacgattacg
     4441 aacctgagct gctgttaatg ccatccaatc agccggtcaa ccagcccatt ctggccgcgg
     4501 ctcagtcctt gcatcgggaa gctaccaagt ggtctagtaa gggcaatgac atcattgcag
     4561 cagccaagcg catggctctg ctgatggctg agatgtctcg gctggtaaga gggggcagtg
     4621 gtaccaagcg ggcactcatt cagtgtgcca aggacatcgc caaggcctca gatgaggtga
     4681 ctcggttggc caaggaggtt gccaagcagt gcacagataa acggattaga accaacctct
     4741 tacaggtatg tgagcgaatc ccaaccataa gcacccagct caaaatcctg tccacagtga
     4801 aggccaccat gctgggccgg accaacatca gtgatgagga gtctgagcag gccacagaga
     4861 tgctggttca caatgcccag aacctcatgc agtctgtgaa ggagactgtg cgggaagctg
     4921 aagctgcttc aatcaaaatt cgaacagatg ctggatttac actgcgctgg gttagaaaga
     4981 ctccctggta ccagtgagaa ttcgcggccg ctcgagtcta gagggcccgc ggttcgaaca
     5041 aaaactcatc tcagaagagg atctgaatat gcataccggt catcatcacc atcaccattg
     5101 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5161 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5221 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5281 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5341 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     5401 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5461 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5521 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5581 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5641 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5701 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5761 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5821 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5881 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5941 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     6001 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6061 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     6121 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     6181 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     6241 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     6301 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     6361 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     6421 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     6481 gggtgcgcgg cctggacgag ctgtacgccg ggtggtcgga ggtcgtgtcc acgaacttcc
     6541 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     6601 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     6661 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6721 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6781 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6841 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6901 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6961 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7021 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7081 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7141 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7201 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7261 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7321 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7381 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7441 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7501 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7561 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7621 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7681 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7741 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7801 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7861 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7921 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7981 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8041 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8101 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8161 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8221 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8281 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8341 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8401 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8461 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8521 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8581 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8641 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8701 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8761 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8821 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8881 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8941 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9001 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9061 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9121 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     9181 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     9241 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     9301 taggcgtttt g
//
