LOCUS       sequence-204867-        9202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSIN - EF-1alpha - MTS - tFd - P2A - MTS - tFNR, vector encoding for
            mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd) 
            and Ferredoxin-NADP(+) oxireductase (FNR), under EF-1alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm-231
FEATURES             Location/Qualifiers
     source          1..9202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..1180
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          233..1171
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1128..1148
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1265..1339
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      1361..1370
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1370..1411
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1721..1777
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1778..1852
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1883..1900
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3082..3102)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3087..3416
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3267..3402
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3426..4025
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3426..3445)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3922..3942
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4318..4551
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4702..4719)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4708..4932
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      4975..4984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5028..5132
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5133..5201,5202..5993)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5351..5370)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6164..6752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6653..6672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             6911..7544
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7591..7716
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8209..8442
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8627..8671
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8958..9075
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      241 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      301 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      361 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      421 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      481 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      541 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      601 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      661 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      721 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      781 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      841 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
      901 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
      961 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1021 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1081 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1141 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattgatc cttcgaacta
     1201 gaattcgaag cttgagctcg agatctgagt ccggtagcgc tagttcgaag gatccgccgc
     1261 caccatgagc gtgctgacgc ccttgctgct tagaggactg accggaagcg cacgccgcct
     1321 gcccgtgcct agggcgaaga tacatagcct gttcgaagcc gccaccatgg gtaagcccat
     1381 tcccaacccg ctgttgggcc tcgatagcac agttaacatg gctacctaca aagtgacact
     1441 ggttcggcct gatggtagcg agacaacaat tgacgtgcct gaggacgaat acatactcga
     1501 cgttgcggaa gagcaagggc tggatctgcc atttagttgc cgagcgggcg cttgcagcac
     1561 ttgtgccggg aagttgctcg aaggggaagt ggatcagtct gatcagtcct ttctggatga
     1621 cgatcagatc gaaaaagggt tcgtgctcac ctgtgtcgct tatccaagga gcgattgtaa
     1681 gattctcact aatcaggagg aggagcttta cggctcaggc gcgacaaatt tttctctcct
     1741 caaacaggct ggggacgtgg aggagaaccc tggccctatg tccgttctta ccccactgtt
     1801 gttgcgggga ctcactggat cggctagacg actccccgtc ccaagggcaa agatccattc
     1861 tctggaattc gccgccacca tgcaccacca ccatcaccac gttaacatgt acaatgcaac
     1921 caactccagg agcagaatgt ttagatatga agtagtagga ctgaggcaga ctgccgaaac
     1981 cgaaaagacc aactatgcca tacgaaatag cggaagccag ttttttaacg taccctatga
     2041 taggatgaat cagttcatgc agcagataac acgctggggc ggaaagatcg tatccattca
     2101 gcccttgaac ggcacagtgg ctcccctggc tgccaccacg gaacccgctg caaataatgg
     2161 cgctgcgccc gtcaaagaga aaaaggttga tataccagtt aacatctatc gcccgaacaa
     2221 cccctgtatt ggaaaagtaa tatccaacga ggaactggtg agggagggcg gtgagggaac
     2281 cgtgaaacac attatttttg atatctctgg aaccgagctc agatatttgg agggccagtc
     2341 tatcgggatc attccggctg ggaccgacgc aaatggtaaa ccacataaac tccggctgta
     2401 ttctatcgcc tcaacgaggc acggggactt tcaggatgac aagaccgtct cactgtgcgt
     2461 tagacgcctc gaatataaag ataaagagac tggcgagacc atttatggcg tgtgctcctc
     2521 ctacctgaat cagctgcagc ctggagacga agtgaagatt actggtcccg ttgggaagga
     2581 aatgctgttg agtgacgacc ctgaagccac catcataatg cttgctaccg gcaccggaat
     2641 tgcccccttt cgcgctttct tgtggcggat gttcaaagaa aacaacccag attatcagtt
     2701 caaaggactt gcctggctgt tttttggcgt cgcctacacc gctaacatcc tgtataaaga
     2761 tgagctggag gccatccagg cccagtatcc tgatcacttt cggcttacat acgcaatttc
     2821 acgagagcag aagactccgg acggtgggaa aatgtacatc caggggcgaa tcgccgaaca
     2881 tgctgacgag atttggcagt tgcttcagaa aaaaaacaca cacgtctata tgtgcggcct
     2941 gaggggcatg gaacctggga tagatgaagc aatgacagca gccgccgcca agaatggcgc
     3001 agattggcag gaatttctta aaggaacatt gaagaaagag ggacggtggc acgtggaaac
     3061 atattaataa gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3121 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3181 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3241 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3301 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3361 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     3421 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     3481 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     3541 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     3601 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     3661 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     3721 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     3781 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     3841 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     3901 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     3961 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4021 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     4081 cgataaaata aaagatttta tttagtctcc agaaaaaggg ggcggccgct cgagacctag
     4141 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     4201 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     4261 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4321 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4381 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4441 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4501 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     4561 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4621 ggccttgaca ttataataga tttagcagga attgaactag gagtggagca cacaggcaaa
     4681 gttctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4741 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4801 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4861 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4921 tgggctctat ggcttctgag gcggaaagaa ccagctgggg gcgcgcccct cgaggccgcc
     4981 atggtcatag ctgtttgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5041 tttgtttatt tttctaaata cattcaaata tgtatccgct cacgagacaa taaccctgat
     5101 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5161 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5221 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5281 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5341 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5401 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5461 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5521 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5581 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5641 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5701 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5761 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5821 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5881 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5941 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6001 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6061 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6301 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6361 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6421 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6481 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6541 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6601 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6661 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6721 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6781 tctggccttt tgcacggacc gccactgcca attacctgtg gtttcattta ctctaaacct
     6841 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     6901 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     6961 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     7021 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     7081 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     7141 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     7201 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     7261 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     7321 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     7381 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7441 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7501 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7561 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7621 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7681 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7741 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7801 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7861 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7921 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7981 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8041 accgcacagc aaccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8101 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8161 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8221 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8281 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8341 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8401 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     8461 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8521 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8581 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8641 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     8701 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     8761 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     8821 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     8881 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccacaaatg
     8941 gcagtattca tccacaattt taaaagaaag ggggggattg gggggtacag tgcaggggaa
     9001 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     9061 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gatcgataag
     9121 ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct
     9181 aggtcttgaa aggagtggga at
//
