LOCUS       sequence-204868-        8592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA3 - CMVmin - PIF3 - VPR expresses PIF3 and VPR transactivator 
            domain , under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm- 226
FEATURES             Location/Qualifiers
     source          1..8592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2536..2685
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             2710..2730
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3160..3516
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             3535..4104
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     promoter        complement(4145..4163)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4146..4163)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4183..4200)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4189..4413
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4459..4887
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4546..4565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4756..4777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4896..4916)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4901..5230
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5081..5216
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5143..5162
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5297..6091
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5351..5370)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5961..5980
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6265..6386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6302..6321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6356..6375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6435..6451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6435..6451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6448..6470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6459..6475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6483..6489,6490..6507,6508..6513))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6528..6549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6666..6683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6837..7425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6917..6936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7596..8387,8388..8456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8219..8238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8457..8561)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 atgcctctgt ttgagctttt caggctcacc aaagctaagc ttgaatctgc tcaagacagg
      961 aacccttctc cacctgtaga tgaagttgtg gagctggtgt gggaaaatgg tcagatatca
     1021 actcaaagtc agtcaagtag atcgaggaac attcctccac cacaagcaaa ctcttctaga
     1081 gctagagaga ttggaaatgg ctcaaagacg actatggtgg acgagatccc tatgtcagtg
     1141 ccatcactaa tgacgggttt gagtcaagac gatgactttg ttccatggtt gaatcatcat
     1201 ccctcccttg atggatattg ctctgatttc ttgcgtgatg tgtcgtctcc tgttactgtc
     1261 aacgagcaag agagtgatat ggcggtaaac caaactgctt tcccgttgtt tcagagaaga
     1321 aaggatggca atgaatcagc tcctgctgct tcttcgtcgc agtataacgg tttccaatcg
     1381 cattctctgt atggaagtga tagagctaga gatcttccta gccaacaaac caatccggat
     1441 cggtttactc agacgcagga accactaatt actagtaaca agcctagttt ggtcaacttt
     1501 tcacatttct tacgccctgc aacttttgcg aagactacta ataataacct tcatgacact
     1561 aaagaaaaga gtcctcaaag cccgccaaat gtgtttcaga ccagagttct tggagctaaa
     1621 gactctgaag ataaggttct taacgagtct gttgcttctg ctacgcctaa agataaccaa
     1681 aaggcttgcc taatatcaga ggactcatgt agaaaagacc aagagagtga aaaagcagtt
     1741 gtatgttctt ctgttggctc gggtaatagt ctcgatggcc catccgaaag tccttcactt
     1801 tctttaaaga gaaagcattc gaatattcaa gacattgact gtcatagtga agatgtggaa
     1861 gaagaatcag gagatggaag aaaggaagca ggtccatctc gaacgggttt gggttcaaag
     1921 agaagccgct ctgcagaagt gcataatctg tctgaaagga gacggcgtga taggatcaac
     1981 gagaagatgc gtgccctgca agaactcatt ccaaactgta acaaggtgga caaagcttcg
     2041 atgctagatg aagccatcga gtatctcaag tcactccaac ttcaagtgca gatcatgtca
     2101 atggcgtctg gttactatct gccaccggcg gttatgttcc caccgggtat ggggcattac
     2161 ccggcagcag ctgctgcaat ggcaatgggt atgggaatgc cttatgcaat gggcttgcct
     2221 gatttgagcc gtggtggttc atcggttaac cacggaccac agttccaagt ctcggggatg
     2281 caacaacaac cagtggcgat gggtattcca cgtgtctctg gtggtggtat ctttgccggt
     2341 tcttcgacga ttggcaatgg ctcgactaga gatttatctg gttctaaaga tcaaacaacg
     2401 acgaataaca acagtaactt gaaaccaata aagagaaaac aggggtcttc tgatcagttt
     2461 tgtggatcgt cggacagcgc cggcagcgcc ggcagcgccg gcagcggatc cgaggctagt
     2521 ggatctggga gagcagatgc tctggatgac tttgatctgg acatgctggg ctccgacgca
     2581 ctcgatgact tcgatctgga tatgcttggc agcgacgcac tggacgactt cgatctggac
     2641 atgttgggat ctgacgcgct ggacgacttc gatctggaca tgctgatcaa ttctcgctca
     2701 tccggttcac caaagaaaaa gaggaaggtt gggagtcagt atcttcccga taccgacgat
     2761 cggcaccgga tagaggaaaa gaggaagcgg acatacgaga ccttcaaatc tatcatgaag
     2821 aagtcaccct tctctggacc caccgatccc agacccccgc cccggcgcat tgccgtaccc
     2881 tcccgcagta gcgcctcagt ccccaagcct gcacctcaac cttatccttt taccagttca
     2941 ctgtctacca ttaattacga cgagtttccg acgatggtgt ttccctctgg ccagatatct
     3001 caggcctctg ccctggcgcc tgcacctcca caggtactgc ctcaggcacc ggcacccgcc
     3061 ccggctcctg caatggtgtc cgccctcgct caggcccccg cacctgtgcc tgtacttgcc
     3121 cctggccccc cgcaggctgt cgctcctcct gcccccaagc ctacccaggc cggggaggga
     3181 actctgtcag aggccttgtt gcagttgcag ttcgatgacg aggacctggg agctcttctg
     3241 ggcaattcta ccgaccccgc cgtcttcact gacctggcaa gcgtggacaa tagcgaattt
     3301 caacagttgc ttaaccaggg tattccggtt gctccgcata ctactgagcc catgctgatg
     3361 gaataccctg aggccatcac cagattggtc accggggcac agcggcctcc ggacccagct
     3421 cccgcccctc tgggcgcccc cggactgcca aacggcctgc tgtccggaga cgaagatttc
     3481 tctagtattg ccgacatgga cttttctgcc cttttgggca gcgggagcgg ttccagagat
     3541 agtcgcgaag gaatgttcct gccaaaaccc gaagcaggca gcgccataag tgacgtgttc
     3601 gagggacggg aagtgtgcca gccaaagagg attagaccat tccacccacc aggctctccc
     3661 tgggctaata ggcccctccc tgcttcattg gcgcctactc cgactggtcc cgtccacgag
     3721 ccagtggggt ctctgactcc tgcacctgtg ccacagcccc tggacccagc cccagctgtc
     3781 acccccgagg ccagccacct gctcgaagat ccagatgagg aaacctccca ggccgtaaag
     3841 gccttgcggg aaatggccga tactgtaatc ccacagaaag aggaggccgc catttgtggg
     3901 caaatggatc tcagtcatcc ccctccaagg ggtcacctgg atgaattgac cacaaccttg
     3961 gaatcaatga cagaagacct caatctggat agccccctga cgccggagct gaacgagatt
     4021 cttgacactt ttctcaacga tgaatgcctc ttgcatgcca tgcatatcag caccggcctg
     4081 tctatttttg atacctcctt gttttgataa gcggccgctc gagcatgcat ctagagggcc
     4141 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
     4201 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4261 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4321 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4381 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4441 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4501 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4561 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4621 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4681 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4741 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4801 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4861 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4921 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4981 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5041 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5101 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5161 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5221 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     5281 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5341 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5401 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5461 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5521 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5581 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5641 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5701 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5761 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5821 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5881 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5941 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6001 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6061 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6121 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6181 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6241 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6301 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6361 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6421 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6481 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6541 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6601 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7261 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7441 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7741 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7921 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7981 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8041 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8101 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8161 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8221 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8281 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8341 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8401 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8461 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8521 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8581 ccacctgacg tc
//
