LOCUS       sequence-204877-        5164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector expressing Cre from rTH promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5164)
  TITLE     AAV plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV.rTH.PI.Cre.SV40
FEATURES             Location/Qualifiers
     source          1..5164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          603..735
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     718..737
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        780..798
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..1878
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    1931..2052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1942..1961)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1996..2015
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2214..2343
                     /label=AAV2 ITR
     primer_bind     complement(2362..2379)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2362..2378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2371..2393)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2520..2975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2607..2626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2817..2838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3001..3105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3106..3174,3175..3966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3324..3343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4137..4725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4626..4645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5013..5034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5049..5054,5055..5072,5073..5079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5087..5103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5092..5114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5111..5127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5111..5127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatca attcctagcg gtctcctgtc ccacagaata ccagccagcc cctgccctac
      241 gtcgtgcctc gggctgaggg tgattcagag gcaggtgcct gtgacagtgg atgcaattag
      301 atctaatggg acggaggcct ttctcgtcgc cctcgctcca tgcccacccc cgcctccctc
      361 aggcacagca ggcgtggaga ggatgcgcag gaggtaggag gtgggggacc cagaggggct
      421 ttgacgtcag cctggccttt aaagagggcg cctgcctggc gagggctgtg gagacagaac
      481 tcgggaccac cagaagcttt attgcggtag tttatcacag ttaaattgct aacgcagtca
      541 gtgcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc
      601 aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga
      661 gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg
      721 cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtactt
      781 aatacgactc actataggct agtcgagaat tgtacacttt acttaaaacc attatctgag
      841 tgtgaaatgt ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca
      901 acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct
      961 gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg
     1021 aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt
     1081 caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt
     1141 catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg
     1201 cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa
     1261 cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata
     1321 cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc
     1381 aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc
     1441 agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact
     1501 aaactggtcg agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg
     1561 ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact
     1621 cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac
     1681 tctggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat
     1741 atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta
     1801 aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg
     1861 ctggaagatg gcgattagcc attaacgcgg cgtggtacct ctagagtcgg gccgcttcga
     1921 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     1981 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2041 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     2101 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt
     2161 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
     2221 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     2281 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     2341 cagccttaat taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     2401 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     2461 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     2521 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2581 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2641 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2701 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     2761 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2821 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2881 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2941 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     3001 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3061 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     4321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     5041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     5101 caatttcaca caggaaacag ctatgaccat gattacgcca gatttaatta aggccttaat
     5161 tagg
//
