LOCUS       sequence-20488-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(14..138)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(146..162)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(146..162)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(168..431)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(251..274)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
       61 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
      121 ttttttgtac aaacttgtga tgggggatcc actagttcta gaatccgtcg aaactaagtt
      181 ctggtgtttt aaaactaaaa aaaagactaa ctataaaagt agaatttaag aagtttaaga
      241 aatagattta cagaattaca atcaatacct accgtcttta tatacttatt agtcaagtag
      301 gggaataatt tcagggaact ggtttcaacc ttttttttca gctttttcca aatcagagag
      361 agcagaaggt aatagaaggt gtaagaaaat gagatagata catgcgtggg tcaattgcct
      421 tgtgtcatca tttactccag gcaggttgca tcactccatt gaggttgtgc ccgttttttg
      481 cctgtttgtg cccctgttct ctgtagttgc gctaagagaa tggacctatg aactgatggt
      541 tggtgaagaa aacaatattt tggtgctggg attctttttt tttctggatg ccagcttaaa
      601 aagcgggctc cattatattt
//
