LOCUS       sequence-204891-        8831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast two hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pCK012
FEATURES             Location/Qualifiers
     source          1..8831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(1344..1649)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1446..1465)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1969..2652)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     2567..2586
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2653..2755)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      2810..2857
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2884..3071
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2939..2963)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3095..3111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3095..3111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3108..3130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3119..3135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3143..3149,3150..3167,3168..3173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3188..3209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3326..3343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3497..4085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3577..3596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4470..4517
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4542..4561
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4749..5543)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4749..5543)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4860..4879)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5470..5489
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5544..5648)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5675..7017
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6638..6685)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7058..7076
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7114..7136)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7236..7255
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7276..7557
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7558..8232
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8335..8790)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8472..8493)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8684..8703
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 actccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ccccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attaatgtga gaccgactgg
     1321 ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg
     1381 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga aaccggcaca
     1441 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa
     1501 agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt
     1561 cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct
     1621 cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga
     1681 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca
     1741 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat
     1801 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata
     1861 cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc
     1921 ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt agtaagccgg
     1981 atccacgcgg cgtttacgcc ccccctgcca ctcatcgcag tactgttgta attcattaag
     2041 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     2101 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     2161 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     2221 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     2281 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     2341 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     2401 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     2461 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2521 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2581 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2641 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     2701 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
     2761 tcaggtctca ttcgtgagct tgatccgtcg acctgcagcg gccgcataac tagcataacc
     2821 ccttggggcc tctaaacggg tcttgagggg ttttttgcgc gcttgcagcc aagctaattc
     2881 cgggcgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
     2941 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac
     3001 tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac
     3061 acctctaccg gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3121 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3181 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3241 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3301 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3361 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3421 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3481 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3541 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3601 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3661 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3721 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3781 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3841 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3901 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3961 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4021 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4081 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4141 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4201 aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc
     4261 ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg
     4321 gggaaaagga agaaacgcgg gcgtattggc cccaatggag tctcggtggg gtttggcccc
     4381 aatggagtct cggtggggta attggcccca atggagtctc ggtggggtat cgacagagtg
     4441 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     4501 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     4561 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     4621 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     4681 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     4741 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     4801 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     4861 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     4921 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     4981 tgagtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     5041 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     5101 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     5161 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     5221 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     5281 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     5341 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     5401 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     5461 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     5521 tgcaatccat cttgttcaat catactcttc ctttttcaat attattgaag catttatcag
     5581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5641 gttccgcgca catttccccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt
     5701 agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
     5761 tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt
     5821 tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     5881 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact
     5941 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag
     6001 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg
     6061 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa
     6121 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa
     6181 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag
     6241 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt
     6301 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct
     6361 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa
     6421 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata
     6481 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc
     6541 ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt
     6601 gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga
     6661 ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc
     6721 aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct
     6781 gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta
     6841 tatgcgtcta tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt
     6901 ccatgcgggg tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca
     6961 ctcctcaatt ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata
     7021 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7081 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     7141 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     7201 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     7261 agtgcaccat agatcaacga cattactata tatataatat aggaagcatt taatagaaca
     7321 gcatcgtaat atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt
     7381 ctttattgaa aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt
     7441 tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat
     7501 tggtgactat tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg
     7561 tctgttatta atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc
     7621 acagaggccg cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg
     7681 cccaatagaa agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa
     7741 gcatataaaa atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct
     7801 aaggaggatg ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga
     7861 gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta
     7921 tttccaaaag actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt
     7981 cccttgtttg attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct
     8041 gactgggttg gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg
     8101 acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc
     8161 ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat
     8221 gctaagaaat aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccga
     8281 tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
     8341 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     8401 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     8461 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     8521 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     8581 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8641 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8701 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8761 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
     8821 ggaagggcga t
//
