LOCUS       sequence-204893-        8577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast two hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG417
FEATURES             Location/Qualifiers
     source          1..8577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1557..1898
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1904..1923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1929..1938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1941..1967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2099..2446)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     primer_bind     2317..2339
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2331..2348
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2332..2348
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2442..2458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2466..2482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2490..2496,2497..2514,2515..2520))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2535..2556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3005..3192
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3060..3084)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(3309..4403)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4404..4809)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(4851..4867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4875..4891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4899..4905,4906..4923,4924..4929))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4944..4965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    5020..5053
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5131..5164
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5233..5250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5404..5992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5484..5503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6163..6954,6955..7023))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6786..6805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7024..7128)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7196..7214
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7252..7274)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7410..8574
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gactccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg
     1981 aggccagtga attaatgtga gaccagacgg tcacagcttg tctgtaagcg gatgccggga
     2041 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2101 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     2161 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     2221 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     2281 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     2341 cggccagtga attcgagctc ggtacctcgc gaatgcatct agatatcgga tcccgggccc
     2401 gtcgactgca gaggcctgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     2461 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2521 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2581 ttccagtcgg gaaacctgtc gtgccagcgg tctcagcttt cgagctgcag atgaatcgta
     2641 gatactgaaa aaccccgcaa gttcacttca actgtgcatc gtgcaccatc tcaatttctt
     2701 tcatttatac atcgttttgc cttcttttat gtaactatac tcctctaagt ttcaatcttg
     2761 gccatgtaac ctctgatcta tagaattttt taaatgacta gaattaatgc ccatcttttt
     2821 tttggaccta aattcttcat gaaaatatat tacgagggct tattcagaag ctttggactt
     2881 cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta ccttgccaga
     2941 aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg acacttctaa
     3001 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     3061 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     3121 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     3181 cacctctacc ggccggtcga aattccccta ccctatgaac atattccatt ttgtaatttc
     3241 gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga
     3301 gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg
     3361 gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact
     3421 gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca
     3481 gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg
     3541 tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca
     3601 gatggcaaca aacccaagga acctgggata acggaggctt catcggagat gatatcacca
     3661 aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg
     3721 gcggcagaat caatcaattg atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt
     3781 tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac
     3841 caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt
     3901 tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc
     3961 tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct
     4021 ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat
     4081 ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt
     4141 ctaacactac ctgtacccca tttaggacca cccacagcac ctaacaaaac ggcatcagcc
     4201 ttcttggagg cttccagcgc ctcatctgga agtgggacac ctgtagcttc gatagcagca
     4261 ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct
     4321 ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg
     4381 acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata tatatattgc
     4441 tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat tgctaaccac ctattggaaa
     4501 aaacaatagg tccttaaata atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc
     4561 atgaacgctt ctctattcta tatgaaaagc cggttccggc gctctcacct ttcctttttc
     4621 tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat taacaaaaaa
     4681 tttccagtca tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc gttatgttga
     4741 ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt
     4801 cccacagttg ggggatctcg actctagcta gaggatcaat tcgtaatcat gtcatagctg
     4861 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4921 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4981 ctgcccgctt tccagtcggg aaacctgtcg tgccagctga taacttcgta taatgtatgc
     5041 tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagctagc ttggctgcag
     5101 gtcgagcggc cgcgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
     5161 ttatcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5221 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5821 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6001 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6061 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6301 gcgagatcca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6781 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6841 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6901 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6961 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7021 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7081 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7141 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     7201 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     7261 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     7321 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     7381 agagcagatt gtactgagag tgcaccataa cgcatttaag cataaacacg cactatgccg
     7441 ttcttctcat gtatatatat atacaggcaa cacgcagata taggtgcgac gtgaacagtg
     7501 agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg
     7561 aagttcctat tccgaagttc ctattctcta gctagaaagt ataggaactt cagagcgctt
     7621 ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc accggactgt
     7681 aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa agtatctctt
     7741 tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt cgctccttga
     7801 acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt actctttaga
     7861 caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg aaaatgtaaa
     7921 catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa ttttctgacc
     7981 aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat ccacatcggt
     8041 atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct tcgctagtaa
     8101 tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata gacaccaaag
     8161 tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag actgcattat
     8221 agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa tagcgctctc
     8281 gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa aatagcgctc
     8341 tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg
     8401 ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag attcttcgtt ggtaaaatag
     8461 cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat
     8521 tagcgctctc gcgttgcatt tttgttctac aaaatgaagc acagatgctt cgttgct
//
