LOCUS       sequence-20491-e         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG424GAL-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(14..138)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(146..162)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(146..162)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(177..618)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(183..206)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    501..618
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
       61 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
      121 ttttttgtac aaacttgtga tgggggatcc actagttcta gaatccgggg ttttttctcc
      181 ttgacgttaa agtatagagg tatattaaca attttttgtt gatactttta ttacatttga
      241 ataagaagta atacaaaccg aaaatgttga aagtattagt taaagtggtt atgcagtttt
      301 tgcatttata tatctgttaa tagatcaaaa atcatcgctt cgctgattaa ttaccccaga
      361 aataaggcta aaaaactaat cgcattatca tcctatggtt gttaatttga ttcgttcatt
      421 tgaaggtttg tggggccagg ttactgccaa tttttcctct tcataaccat aaaagctagt
      481 attgtagaat ctttattgtt cggagcagtg cggcgcgagg cacatctgcg tttcaggaac
      541 gcgaccggtg aagacgagga cgcacggagg agagtcttcc ttcggagggc tgtcacccgc
      601 tcggcggctt ctaatccgta ctagagctcc agcttttgtt ccct
//
