LOCUS       sequence-204924-       13808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert a single copy of a construct at a site on Chr I, 
            corresponding to the Shigella flexneri protein OspF (a MAP kinase 
            inhibitor) expressed specifically in the intestine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Lee SH, Omi S, Thakur N, Taffoni C, Belougne J, Engelmann I, Ewbank 
            JJ, Pujol N
  TITLE     Modulatory upregulation of an insulin peptide gene by different 
            pathogens in C. elegans.
  JOURNAL   Virulence. 2018 Dec 31;9(1):648-658. doi: 
            10.1080/21505594.2018.1433969.
  PUBMED    29405821
REFERENCE   2  (bases 1 to 13808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/21505594.2018"; journalName: "Virulence"; date: 
            "2018-12-31- 31"; volume: "9"; issue: "1"; pages: "648-658"
COMMENT     Sequence Label: pSO9
FEATURES             Location/Qualifiers
     source          1..13808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3415..3431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3415..3431)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3428..3450)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3439..3455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3463..3469,3470..3487,3488..3493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3508..3529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3590..4178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3670..3689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      4289..4798
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4376..4395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4582..4603
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4840..5631,5632..5700))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5463..5482
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5701..5805)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6011..6033
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6026..6042
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7607..7640)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(7640..7651)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(9073..9396)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    11314..11338
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11341..12366
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             11341..12366
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(12367..12391)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12744..12777)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     13147..13163
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tttttatagt ttgtgaaatt ttttatactt ccacatttca tgaacagttt ggtttggcca
       61 catgactaag gcgtgaccca catagaaaac atcaggtgcc tttgaagcaa tatttcacat
      121 cttcgcaaag ctaaggtcat tgttattttt gccatttcat gactataatg gcatcatttt
      181 attcccccaa tttaaccaaa agtcagatgc aaaatgttga aagtgttgca atttcaaagt
      241 agaaaatgta actaaactgt caaagaaaat tgtcatactt tgaccagaaa ccatctcccc
      301 atccacctcc cctcatcgga gccacccgcc aatcccaaaa tggtacctaa cattactgac
      361 actctcccgc actcaacaat tgacacctgt ctcaatgact gataagcatg ctgagtctct
      421 tcaatccgca tggtctctaa gctactttca gaattgcaac atgctgcgat ggacatcttg
      481 tcgaagttta agagtatgta taaaaggaca cgagcttcat gtattcttca ctcggtcact
      541 tagatcgatc aatcactatg cccataaaaa agccctgtct aaaactaaat ttagattctt
      601 tgaatgttgt gaagtctgaa ataccgcaga tgctttctgc gaatgaaaga ttgaaaaata
      661 acttcaatat attgtacaac caaatacggc aatacccagc gtactatttc aaagtagcat
      721 cgaacgtgcc aacttactct gatatatgtc aatttttttc tgtcatgtac caaggattcc
      781 agattgtgaa ccatagtgga gatgtgttta ttcatgcatg tcgtgaaaat cctcagagta
      841 agggggattt tgttggggac aagtttcata ttagtatagc tagggagcaa gttcctctgg
      901 cattccaaat tttgtctggt ttattatttt cagaggacag tcctatagat aaatggaaga
      961 taactgatat gaatcgagtt tctcagcaat ctcgtgtggg gataggagct cagtttacgc
     1021 tatatgtaaa atcagatcag gaatgctcgc aatatagtgc tttattactt cataaaatac
     1081 gacaatttat aatgtgtctc gagtctaatc tattaagaag caaaatcgct cccggggagt
     1141 atccggcgtc agatgttaga cctgaagact ggaaatatgt cagctatcgt aatgaactac
     1201 gaagcgatcg agatggaagt gaaaggcaag agcaaatgtt acgagaggaa ccattttatc
     1261 gattgatgat agagtagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt
     1321 caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa
     1381 aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt
     1441 gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc
     1501 cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg
     1561 ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct
     1621 tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct
     1681 ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca
     1741 atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt
     1801 gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac
     1861 gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtcgatgc
     1921 gcgatgcaag tcggcgattt ctttgaagtt ttgtcaaaag aagagacaag gacttgatag
     1981 atacgctcat cagagtgaga ttcattgcaa atagtgtgta aatcgtggag agacgagaga
     2041 aaaggtgtga gaagcttaga agataaagaa taggaagaag aacatatctc atttcctatt
     2101 ttctctaatt tttcgttcat ttttctagac attacccatt tgatggtttt tcaatatggt
     2161 tatcagggta ctcattttgg tagttttcaa agcaatttgg agtttttttt taaattgtca
     2221 actacaaaaa cgcagcttaa aggtccaaat atcatagtat aaaaattctt taaaaaattc
     2281 caaattttat atgatttttt gaaaattgga agaatgttag tttttgccat ttttttggaa
     2341 gtttttctcc ggattttatg gtacttatat ttcaaatata aatttttgta ggaattcgag
     2401 tatgcgagag agctatagtt tactaaaaaa aagtggcaaa aactcagtaa ttgccacttt
     2461 tttcaaaaat gttttgaaaa attttactat gatatttggc cattttggca ccatatgtac
     2521 gttacttttt acgaaacact ctaatttttg aaatttgagt ttcatgttta atcttttgta
     2581 taaaattttt atggtccttt tgtgacagga taaaaaattg caaaaaaata gaaaactttg
     2641 tttcgtgaaa acacctagtt taaaaattgt gggccactta gcgattctag tcaaaaatca
     2701 gacaaatgtc agctgccgta actttttccg gatcagggtt atcaaatttt aattttaatt
     2761 gtaaatgtag aaaaatgatc ggcaacattt tgctaattga aaatattttg aaaattgcta
     2821 tagtacatat taatctagta ttaatatagt attaatgtta taatatcagt tatgtgtttg
     2881 aataactcaa gcgtaagttt ccgtaaaata tctttaaatt ttggtaattt tttacggttt
     2941 ggtataaatt tttgcatttt ttgattttct acactttttt tcgatttcgc attttccgaa
     3001 tttctctgaa ataaatcgaa caatcggaaa attttcgagg gcacccttag ccatgcagct
     3061 caggaatttg actttatgtt cccattcaaa tttttttaaa attaaataaa atgctaattg
     3121 taagataaat tacaaaaatt attaaatctc atcatcctta tgatgatagt gcagtgcatg
     3181 aatatatttt tccaaatagg atatgattcg tcgagtagct tcacgtaatc cgttttcttc
     3241 aatcaccatt tcgaactgca attttttttt tggatttttg taacaatatt gtcataaatt
     3301 gtgattttga tattttgcgt agagcatcag aacctttctg ctttgaattt taatgggatt
     3361 aaaaattata aaaaacttgc accctaatta aagcgcgata cctggtgacc catggtcata
     3421 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3481 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattac atgtgagcaa
     3541 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3601 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3661 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3721 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3781 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3841 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3901 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3961 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4021 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4081 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4141 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4201 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4261 caaaaaggat cttcacctag atccttttac gcgccctgta gcggcgcatt aagcgcggcg
     4321 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4381 ttcgctttct tcccttcctt tctcgccacg ttcgctttcc ccgtcaagct ctaaatcggg
     4441 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4501 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4561 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4621 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4681 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     4741 aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca accggggtaa
     4801 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4861 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4921 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4981 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5041 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5101 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5161 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5221 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5281 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5341 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5401 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     5461 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggga aacgttcttc
     5521 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5581 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5641 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5701 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5761 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5821 agtgccacct gacgtagtta acaaaaaaaa gcccgccgaa gcgggcttta ttaccaagcg
     5881 aagcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     5941 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     6001 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gttgatcagt catccaagtg
     6061 gggatcagga agaagttgat aaagtgattc aaaaactcga ggagctcaac acagcgatga
     6121 acccaatttt gagagcaaca ctgacgaaga actatgacgc gagggtaggc actaccttga
     6181 agctatccac ctgcctgcct gccgcctatt tccatgtgca gtaggaacgt gtgtaggtat
     6241 aggcaggcat tatcgcgtct cctttcgaac tacctagtac ctagtaggta gtttgaaggg
     6301 aggcgcgata atgcctgcct atgtctacac atgtgcctac tgcgcatgga aataggcggt
     6361 agtcaggcag gtagatagcc tcataaaatt caatacttcc ttttcagaaa gaatgctgga
     6421 atgtattcaa agaggagtca ctcttcccat tgctcagaga atatgaagag ttgctcggcg
     6481 agcaaaagtt catgattgga gatcgatatt catgggctga catcgctgtc gtcgagtttc
     6541 taactcggtg tcagcagtgc tacgatagct tctatctggc tcatttcgag aggctccgaa
     6601 ctttttgcca gacttttgag tctcttccgc acattcgtcc ctacattcag ggacgtgttg
     6661 attcatttat ctaatttttt aaaatggata tttttcgttt tttatttgtt tgttttgctg
     6721 ataattcaat aaagttacgc aacgaatttt agacatttat gaaaaccgaa aatcgacaaa
     6781 aatattttaa gaaaaatagc agcaaaattc aatattttat caaattttgt ttatggtaat
     6841 gagaaagaaa acatacaata tttttctaag aagaagaaaa atgaccagaa ttttaagatt
     6901 tttctcgaat atacgaaaaa ttgataaact aaaattttcg atttttccat ttattttgaa
     6961 aaaaaaactc ttgacggttt tcatcatgcg aagaggtgag aaatattctg aaagttatgc
     7021 attttgaaaa aattgcaaaa aaatacgtta aaattttttg atctattgtt tttttttgaa
     7081 aaccggaatt taaaatggaa ttttttttca aaaattaaaa ttaaaattta aatatttcta
     7141 gtgtttggat agtttttcaa aagctagatt taacccgcga gatgtcgacc gctagtgtag
     7201 cttacagatg gctgaagact tcgcaaaaat cttatcttag aaacccgcga ggtgtcggcc
     7261 gctaaaactt tgtaaacctg ccaacaaaat gcttcgcatt gtctgaacag atgaaacctt
     7321 gaaacaaaac atatacaatt gaaatcccgt aaagtgtcgg gcgcaagaac tgcgattagt
     7381 tgtataattg gactagctca aatttgacca aaatctcatt tttagacctc taagatgtcg
     7441 gccactataa ctttattcaa cttgaaactg caaaaaaact tatcaactgt atagattcaa
     7501 gtattatgtc aagtattggt tttagagcta cagtacactc tgataacaaa atctgcagaa
     7561 atacctccct gtcaattccc aaaatacggt agcaaactca cttcgtataa cttcgtatag
     7621 tataccttat acgaagttat ctgccctcga ttgtctatct tccattctca ccaagtttca
     7681 acaactttat tcagtcattc ttctctggtt catttcccaa ccaatcttcc cgaataaagc
     7741 ccatctgtcc tttcggccac attcctcacc tacatccgta ctccttatct cccgttctta
     7801 tccagtcttt tatcgtctct gcgtctcgca cactgtctcg tcggtaatgt aaaaaaaaaa
     7861 caaaaaaaac ttgaaaactt gcggaaagat ttcctaataa aaggagacga atccggtagg
     7921 aaatcgacaa gtctctgctt ctcgaagact aggtgagtta catttctttg gccgaattat
     7981 tttgaaatcc taaatttgat aatttcagag atgtctgtaa aacttgcgtg ttatgtgacg
     8041 gcttcggtca ctgtcgccac tcttatggta tgcttcatga ccatgtcaac catctactcg
     8101 gaggttgatg gattcagaga gaagcttgat accgagatga atgtgttcag agtgagttaa
     8161 attatatttt gaattttaaa taatttttaa ttttctagca atctaccaat ggattgtgga
     8221 aggacatagt tgtcatcgga agatctagca agcgtgtccg ttgtcaatat gaagagacca
     8281 acgctacccc aactccacat gctgatggat ccccatctgc tccaccaggt caaccaccag
     8341 cagttccacc agtcttcaac cagccaaaga ctccaaatgg agccaatgga aatggaccaa
     8401 cctgcaactg caatgctgat aacaagtgcc cagctggacc atccggacca aagggagttc
     8461 caggagttcc aggactcgac ggagttccag gacttgacgg tgttccagga gttggagctg
     8521 atgatatcgc tccacaacgc gagtctgtcg gatgcttcac ttgcccacaa ggaccagttg
     8581 gaccaccagg agctcttgga agaccaggac cacgtggact tccaggacca agaggacaaa
     8641 atggaaaccc aggaagagat ggacaaccag gacatccagg agagcaagga tcatccggtc
     8701 aaatcggaaa gatcggagag ccaggaccac caggagagaa gggacgcgac gccgagcatc
     8761 caatcggaag accaggacca aagggaccaa gaggagatca aggaccaaca ggaccagctg
     8821 gacagaacgg tcttcacgga ccaccaggag agccaggaac cgttggacca gaaggaccat
     8881 ctggaaagca aggacgtcaa ggaccagacg gaacccaggg agagactgga ccagacggaa
     8941 gaccaggaaa ggatgccgag tactgccagt gcccagacaa gtctccacca tcagaggctg
     9001 tcaacgccaa ccgtggatac agaaatatct aaattgttgg tgttttctaa taaaatattt
     9061 gagattcagt gagacttttt tcttggcggc acaataaagt ttcctctttt tgttgggtca
     9121 ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt tctcaacctt tcgggtttaa
     9181 agcacttcaa aatggaattg aagagagatt gtgaaagtac atcagacaga atcacatttt
     9241 gatagcattc acttcactca gatgcaaact tttcagtgac tataaaaagt cataaaataa
     9301 taacattaaa aaagcaaata aattagcgag agaatttttt gacaaaaaga aagaatagtg
     9361 atagagaaga agggaatgct tgaaaggatt ttgcatttat cttaatcacc gtcttcgaga
     9421 agacgaacca tggcgccagt ttcagaatcc aagtttctaa tatagttcat cacaatgttg
     9481 acgttggtcc aacctccggc ttgcataatc tctgggatgg aaactccagc gcgagccatg
     9541 tcacgggcag ctccgacacg ggcggaatgt ccagaccaag ccaagtaacg ttgtccggaa
     9601 tcatccttgg ctccgtagat gagacggtgg gtagcttcga agattcctga aaatttattt
     9661 tcagcttatt cttagctaat atgttgttta aacttacctt caagagctcg agtcgagagt
     9721 tgtgatgttg cactcggggc agcaactccg ttcttgcgaa cacgacagaa caggtagttg
     9781 tttggatcat cggcgactcc ggaaaccgaa atccagcgct caacgagctt ggtaactcca
     9841 agggagagcg ctttttcgac tccggcagtc gagaccagcg tttttgtacg tccgatgtgg
     9901 atgagcattc ttcctccgtc tgtgcgacta atatccttga cgcgaatgcg ggcaatttcg
     9961 gcgatacgga gaagtgtgtt gtaagcaatt ccgaggaagg cgaggtttcg gatgtcctgg
    10021 caacgatcgg aattttccat aagagaacgg acctgatcga agtcggttct gaaaatttcg
    10081 tcaagtctta ggattgaata cattaatatt taaacttacc tttcgaaggc gagggcttgc
    10141 ttggctctct ctccagcatc aacattttcc ttgcggatac gtctcattac gagagatacg
    10201 gcattagaat ctgatgggcg tggaagtcca gatcggcgat ggagcatatt gagctgtccg
    10261 agatgttgtt ggatcgtctt caccgccaat ccacgagctt ggaggtagag caagtagtca
    10321 cgaacgtctt ctggctcggc tgggaaccac ttgcggttgt taagcttgca ccaggcagcc
    10381 caagaacggc acactgagag aagcatcttc caggtgtgct cggagaaggc ttgtctgtca
    10441 cggaacatat ccatgaggtt cttgcgaact tcatcacttg tcgcgtcaac tggaagtgct
    10501 ggaagatttt gatgaacagt gagaagattt gacatatttt cgaagttttt tagatgcact
    10561 agaacaaagc gtgttggctt cctctgagcc cgctttcctt atatacccgc attctgcagc
    10621 cttacagaat gttctagaag gtcctagatg cattcgtttg aaaatactcc cggtgggtgc
    10681 aaagagacgc agacggaaaa tgtatctggg tctctttatt gtgtacacta cttttccatg
    10741 taccgaatgt gagtcgccct ccttttgcaa caagcagctc gaatgttcta gaaaaaggtg
    10801 gaaaatagta taaataccgt tgaaaataaa taccgaacaa catttgctct aattgtgaaa
    10861 ttagaaatct tcaaactata atcatgtcac tttaccacta tttccgtcca gctcaacgtt
    10921 ccgtttttgg tgatcatttt tgctttcgtc gtaaatctac acacgcgtct cttccgtgcg
    10981 agagtccaag ccagcagcca aattcgttga ctgagtattc aacgtttata cgttgtcggc
    11041 aacgagaaat aggaaaatgc atcgggaaat gttctttttt cgattttttc caaggttttg
    11101 acaaatttta ccacgaattt tgctatgttt tcaattaaaa aatatgttat tcaactgttt
    11161 ctatgaggaa aataaggctt tgcatgtaat tttcttattc agcataattt ttaattaatt
    11221 tgaattttct gtcctaacgt ttattttgtt ttcttggtta tgactgatct gaaattaatt
    11281 tttgaatttt aaggtaatat gtcaggcggt gccgcaagtt tgtacaaaaa agcaggctcc
    11341 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
    11401 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
    11461 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
    11521 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
    11581 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
    11641 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
    11701 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
    11761 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
    11821 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
    11881 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
    11941 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
    12001 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
    12061 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
    12121 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
    12181 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
    12241 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
    12301 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
    12361 gaatagaccc agctttcttg tacaaagtgg gtccaattac tcttcaacat ccctacatgc
    12421 tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt
    12481 ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat
    12541 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa
    12601 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata
    12661 aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt
    12721 tcagtttatg accgcaattt ttaataactt cgtatagtat accttatacg aagttatact
    12781 agtatatatt tttatgtttt cttttaaacc ttcctccata tttctgaatt gaatgttgtt
    12841 gcatattgtg tgtccttatt tcatttccat catccttttg tgtgtttccc ccttccacca
    12901 attcccccca cgtcctaatg aaatgaaagc gagaagataa ataaacttgc acattgtgtt
    12961 cagatgagag gagcggaata cgaattggga gacggaaaga gaaaaaaaaa tgtttagatt
    13021 tggattgaat taatttttta gaaagcaaga aaaaaaacac aaagagaata aataaataat
    13081 aggaaaaaaa acaaatattc agtatttaaa atttgaaaat tcaacgacgt tggcgtcgat
    13141 catcctgtaa aacgacggcc agtgcagagc atagttggaa atctcaccaa attttgaaat
    13201 ttgaaaatga gttagtaatt tttgaacaat ctgccagaga ttaattaaag ataaggaata
    13261 tccacaaatt acaaatattg gatattaaat atgatttcac gtcataactg agatttgtat
    13321 aatatcataa ttgtacggaa atatttaggt attagtattt cagctcacaa tcagactttt
    13381 taatgaaaac ttaattcaaa aggggatatc ttaaatatca ttacaaaacc ttgttagcag
    13441 cagaactaga aagtttaaaa aaacaccgat ttgtggtgca taatgtacaa acatccattg
    13501 acaacatttc tttaaaaaat aattttttac taaaacattt tgaagtcaaa attaaatttt
    13561 ttcaaaaata aaaaatttac gggcctctaa agtagtggaa aatgcacagc aaagtattca
    13621 gatagacttt tttcttgttc aaaatgcacc tttgccacta aaccgaaatt cttgccttgt
    13681 tcattaattg tttcaagttt gtaggatttt tagaactttg aaatatctaa attatttgta
    13741 ttctgaaaaa tattatcatt tcgcaacaaa aaactagaag cagaataatt tattgtgaat
    13801 cttgctat
//
