LOCUS       sequence-204930-       13274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert a single copy of a construct at a site on Chr I, 
            corresponding to the Shigella flexneri protein OspF (a MAP kinase 
            inhibitor) expressed specifically in the epidermis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13274)
  AUTHORS   Lee SH, Omi S, Thakur N, Taffoni C, Belougne J, Engelmann I, Ewbank 
            JJ, Pujol N
  TITLE     Modulatory upregulation of an insulin peptide gene by different 
            pathogens in C. elegans.
  JOURNAL   Virulence. 2018 Dec 31;9(1):648-658. doi: 
            10.1080/21505594.2018.1433969.
  PUBMED    29405821
REFERENCE   2  (bases 1 to 13274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/21505594.2018"; journalName: "Virulence"; date: 
            "2018-12-31- 31"; volume: "9"; issue: "1"; pages: "648-658"
COMMENT     Sequence Label: pSO7
FEATURES             Location/Qualifiers
     source          1..13274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..36
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     29..45
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1610..1643)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1643..1654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3076..3399)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5317..5341
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5344..6369
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5344..6369
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6370..6394)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6747..6780)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7150..7166
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10692..10708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10692..10708)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10705..10727)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10716..10732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10740..10746,10747..10764,10765..10770))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10785..10806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10867..11455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10947..10966)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      11566..12075
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11653..11672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11859..11880
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(12117..12908,12909..12977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12740..12759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12978..13082)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagttgatc agtcatccaa
       61 gtggggatca ggaagaagtt gataaagtga ttcaaaaact cgaggagctc aacacagcga
      121 tgaacccaat tttgagagca acactgacga agaactatga cgcgagggta ggcactacct
      181 tgaagctatc cacctgcctg cctgccgcct atttccatgt gcagtaggaa cgtgtgtagg
      241 tataggcagg cattatcgcg tctcctttcg aactacctag tacctagtag gtagtttgaa
      301 gggaggcgcg ataatgcctg cctatgtcta cacatgtgcc tactgcgcat ggaaataggc
      361 ggtagtcagg caggtagata gcctcataaa attcaatact tccttttcag aaagaatgct
      421 ggaatgtatt caaagaggag tcactcttcc cattgctcag agaatatgaa gagttgctcg
      481 gcgagcaaaa gttcatgatt ggagatcgat attcatgggc tgacatcgct gtcgtcgagt
      541 ttctaactcg gtgtcagcag tgctacgata gcttctatct ggctcatttc gagaggctcc
      601 gaactttttg ccagactttt gagtctcttc cgcacattcg tccctacatt cagggacgtg
      661 ttgattcatt tatctaattt tttaaaatgg atatttttcg ttttttattt gtttgttttg
      721 ctgataattc aataaagtta cgcaacgaat tttagacatt tatgaaaacc gaaaatcgac
      781 aaaaatattt taagaaaaat agcagcaaaa ttcaatattt tatcaaattt tgtttatggt
      841 aatgagaaag aaaacataca atatttttct aagaagaaga aaaatgacca gaattttaag
      901 atttttctcg aatatacgaa aaattgataa actaaaattt tcgatttttc catttatttt
      961 gaaaaaaaaa ctcttgacgg ttttcatcat gcgaagaggt gagaaatatt ctgaaagtta
     1021 tgcattttga aaaaattgca aaaaaatacg ttaaaatttt ttgatctatt gttttttttt
     1081 gaaaaccgga atttaaaatg gaattttttt tcaaaaatta aaattaaaat ttaaatattt
     1141 ctagtgtttg gatagttttt caaaagctag atttaacccg cgagatgtcg accgctagtg
     1201 tagcttacag atggctgaag acttcgcaaa aatcttatct tagaaacccg cgaggtgtcg
     1261 gccgctaaaa ctttgtaaac ctgccaacaa aatgcttcgc attgtctgaa cagatgaaac
     1321 cttgaaacaa aacatataca attgaaatcc cgtaaagtgt cgggcgcaag aactgcgatt
     1381 agttgtataa ttggactagc tcaaatttga ccaaaatctc atttttagac ctctaagatg
     1441 tcggccacta taactttatt caacttgaaa ctgcaaaaaa acttatcaac tgtatagatt
     1501 caagtattat gtcaagtatt ggttttagag ctacagtaca ctctgataac aaaatctgca
     1561 gaaatacctc cctgtcaatt cccaaaatac ggtagcaaac tcacttcgta taacttcgta
     1621 tagtatacct tatacgaagt tatctgccct cgattgtcta tcttccattc tcaccaagtt
     1681 tcaacaactt tattcagtca ttcttctctg gttcatttcc caaccaatct tcccgaataa
     1741 agcccatctg tcctttcggc cacattcctc acctacatcc gtactcctta tctcccgttc
     1801 ttatccagtc ttttatcgtc tctgcgtctc gcacactgtc tcgtcggtaa tgtaaaaaaa
     1861 aaacaaaaaa aacttgaaaa cttgcggaaa gatttcctaa taaaaggaga cgaatccggt
     1921 aggaaatcga caagtctctg cttctcgaag actaggtgag ttacatttct ttggccgaat
     1981 tattttgaaa tcctaaattt gataatttca gagatgtctg taaaacttgc gtgttatgtg
     2041 acggcttcgg tcactgtcgc cactcttatg gtatgcttca tgaccatgtc aaccatctac
     2101 tcggaggttg atggattcag agagaagctt gataccgaga tgaatgtgtt cagagtgagt
     2161 taaattatat tttgaatttt aaataatttt taattttcta gcaatctacc aatggattgt
     2221 ggaaggacat agttgtcatc ggaagatcta gcaagcgtgt ccgttgtcaa tatgaagaga
     2281 ccaacgctac cccaactcca catgctgatg gatccccatc tgctccacca ggtcaaccac
     2341 cagcagttcc accagtcttc aaccagccaa agactccaaa tggagccaat ggaaatggac
     2401 caacctgcaa ctgcaatgct gataacaagt gcccagctgg accatccgga ccaaagggag
     2461 ttccaggagt tccaggactc gacggagttc caggacttga cggtgttcca ggagttggag
     2521 ctgatgatat cgctccacaa cgcgagtctg tcggatgctt cacttgccca caaggaccag
     2581 ttggaccacc aggagctctt ggaagaccag gaccacgtgg acttccagga ccaagaggac
     2641 aaaatggaaa cccaggaaga gatggacaac caggacatcc aggagagcaa ggatcatccg
     2701 gtcaaatcgg aaagatcgga gagccaggac caccaggaga gaagggacgc gacgccgagc
     2761 atccaatcgg aagaccagga ccaaagggac caagaggaga tcaaggacca acaggaccag
     2821 ctggacagaa cggtcttcac ggaccaccag gagagccagg aaccgttgga ccagaaggac
     2881 catctggaaa gcaaggacgt caaggaccag acggaaccca gggagagact ggaccagacg
     2941 gaagaccagg aaaggatgcc gagtactgcc agtgcccaga caagtctcca ccatcagagg
     3001 ctgtcaacgc caaccgtgga tacagaaata tctaaattgt tggtgttttc taataaaata
     3061 tttgagattc agtgagactt ttttcttggc ggcacaataa agtttcctct ttttgttggg
     3121 tcactcaacg aacacaatac aaatatttga gcgctcgcat ttttctcaac ctttcgggtt
     3181 taaagcactt caaaatggaa ttgaagagag attgtgaaag tacatcagac agaatcacat
     3241 tttgatagca ttcacttcac tcagatgcaa acttttcagt gactataaaa agtcataaaa
     3301 taataacatt aaaaaagcaa ataaattagc gagagaattt tttgacaaaa agaaagaata
     3361 gtgatagaga agaagggaat gcttgaaagg attttgcatt tatcttaatc accgtcttcg
     3421 agaagacgaa ccatggcgcc agtttcagaa tccaagtttc taatatagtt catcacaatg
     3481 ttgacgttgg tccaacctcc ggcttgcata atctctggga tggaaactcc agcgcgagcc
     3541 atgtcacggg cagctccgac acgggcggaa tgtccagacc aagccaagta acgttgtccg
     3601 gaatcatcct tggctccgta gatgagacgg tgggtagctt cgaagattcc tgaaaattta
     3661 ttttcagctt attcttagct aatatgttgt ttaaacttac cttcaagagc tcgagtcgag
     3721 agttgtgatg ttgcactcgg ggcagcaact ccgttcttgc gaacacgaca gaacaggtag
     3781 ttgtttggat catcggcgac tccggaaacc gaaatccagc gctcaacgag cttggtaact
     3841 ccaagggaga gcgctttttc gactccggca gtcgagacca gcgtttttgt acgtccgatg
     3901 tggatgagca ttcttcctcc gtctgtgcga ctaatatcct tgacgcgaat gcgggcaatt
     3961 tcggcgatac ggagaagtgt gttgtaagca attccgagga aggcgaggtt tcggatgtcc
     4021 tggcaacgat cggaattttc cataagagaa cggacctgat cgaagtcggt tctgaaaatt
     4081 tcgtcaagtc ttaggattga atacattaat atttaaactt acctttcgaa ggcgagggct
     4141 tgcttggctc tctctccagc atcaacattt tccttgcgga tacgtctcat tacgagagat
     4201 acggcattag aatctgatgg gcgtggaagt ccagatcggc gatggagcat attgagctgt
     4261 ccgagatgtt gttggatcgt cttcaccgcc aatccacgag cttggaggta gagcaagtag
     4321 tcacgaacgt cttctggctc ggctgggaac cacttgcggt tgttaagctt gcaccaggca
     4381 gcccaagaac ggcacactga gagaagcatc ttccaggtgt gctcggagaa ggcttgtctg
     4441 tcacggaaca tatccatgag gttcttgcga acttcatcac ttgtcgcgtc aactggaagt
     4501 gctggaagat tttgatgaac agtgagaaga tttgacatat tttcgaagtt ttttagatgc
     4561 actagaacaa agcgtgttgg cttcctctga gcccgctttc cttatatacc cgcattctgc
     4621 agccttacag aatgttctag aaggtcctag atgcattcgt ttgaaaatac tcccggtggg
     4681 tgcaaagaga cgcagacgga aaatgtatct gggtctcttt attgtgtaca ctacttttcc
     4741 atgtaccgaa tgtgagtcgc cctccttttg caacaagcag ctcgaatgtt ctagaaaaag
     4801 gtggaaaata gtataaatac cgttgaaaat aaataccgaa caacatttgc tctaattgtg
     4861 aaattagaaa tcttcaaact ataatcatgt cactttacca ctatttccgt ccagctcaac
     4921 gttccgtttt tggtgatcat ttttgctttc gtcgtaaatc tacacacgcg tctcttccgt
     4981 gcgagagtcc aagccagcag ccaaattcgt tgactgagta ttcaacgttt atacgttgtc
     5041 ggcaacgaga aataggaaaa tgcatcggga aatgttcttt tttcgatttt ttccaaggtt
     5101 ttgacaaatt ttaccacgaa ttttgctatg ttttcaatta aaaaatatgt tattcaactg
     5161 tttctatgag gaaaataagg ctttgcatgt aattttctta ttcagcataa tttttaatta
     5221 atttgaattt tctgtcctaa cgtttatttt gttttcttgg ttatgactga tctgaaatta
     5281 atttttgaat tttaaggtaa tatgtcaggc ggtgccgcaa gtttgtacaa aaaagcaggc
     5341 tccatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     5401 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     5461 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     5521 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     5581 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     5641 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     5701 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     5761 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     5821 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     5881 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     5941 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     6001 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     6061 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     6121 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     6181 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     6241 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     6301 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     6361 aaggaataga cccagctttc ttgtacaaag tgggtccaat tactcttcaa catccctaca
     6421 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa
     6481 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
     6541 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
     6601 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg
     6661 ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac
     6721 gtttcagttt atgaccgcaa tttttaataa cttcgtatag tataccttat acgaagttat
     6781 actagtatat atttttatgt tttcttttaa accttcctcc atatttctga attgaatgtt
     6841 gttgcatatt gtgtgtcctt atttcatttc catcatcctt ttgtgtgttt cccccttcca
     6901 ccaattcccc ccacgtccta atgaaatgaa agcgagaaga taaataaact tgcacattgt
     6961 gttcagatga gaggagcgga atacgaattg ggagacggaa agagaaaaaa aaatgtttag
     7021 atttggattg aattaatttt ttagaaagca agaaaaaaaa cacaaagaga ataaataaat
     7081 aataggaaaa aaaacaaata ttcagtattt aaaatttgaa aattcaacga cgttggcgtc
     7141 gatcatcctg taaaacgacg gccagtgcca tttgaaaatt tgcaccaatg tattatttaa
     7201 tttttttttt cgaaaattta acgcattttc tctctaaaaa ctcgaaattt agtgtgttct
     7261 aaacaacagt aagcatacaa tacctttgtt caaaattgac gtgctttctg aaccaatatg
     7321 gttagtttca aaaatttttg tattatagga tagaaatatt tggaaataaa ttttaaaacc
     7381 aaacttatgc ctttctcttt tagtatccca gctaggtaat attttagtat ttgcccaaat
     7441 ccttgaagta aggagtatat aatttttgaa aaacaataaa actccagata attcatagtt
     7501 ttttctcgaa agaaaatttt tgagattagt tattgaactt catttttgaa cattattcgt
     7561 tgaaaaacac tcgctttgtc tttattttca aaaaaattcc gatttcccca accagaaaaa
     7621 aaaacagata gaagaaattt ctccttaaat ttcattgtcc atctctcttg gaaacacatt
     7681 atctatcaaa tgaaaaacgc attttttttc ctggcagaaa aatgaaattg gttagattac
     7741 actggttagg tttgaaggtg taactttcgc tttctcagca actttcagta taaaaggaaa
     7801 cggtcaccat ttagaaagac atcagttcat caacatgccc ataaaaaagc cctgtctaaa
     7861 actaaattta gattctttga atgttgtgaa gtctgaaata ccgcagatgc tttctgcgaa
     7921 tgaaagattg aaaaataact tcaatatatt gtacaaccaa atacggcaat acccagcgta
     7981 ctatttcaaa gtagcatcga acgtgccaac ttactctgat atatgtcaat ttttttctgt
     8041 catgtaccaa ggattccaga ttgtgaacca tagtggagat gtgtttattc atgcatgtcg
     8101 tgaaaatcct cagagtaagg gggattttgt tggggacaag tttcatatta gtatagctag
     8161 ggagcaagtt cctctggcat tccaaatttt gtctggttta ttattttcag aggacagtcc
     8221 tatagataaa tggaagataa ctgatatgaa tcgagtttct cagcaatctc gtgtggggat
     8281 aggagctcag tttacgctat atgtaaaatc agatcaggaa tgctcgcaat atagtgcttt
     8341 attacttcat aaaatacgac aatttataat gtgtctcgag tctaatctat taagaagcaa
     8401 aatcgctccc ggggagtatc cggcgtcaga tgttagacct gaagactgga aatatgtcag
     8461 ctatcgtaat gaactacgaa gcgatcgaga tggaagtgaa aggcaagagc aaatgttacg
     8521 agaggaacca ttttatcgat tgatgataga gtagcatctc gcgcccgtgc ctctgacttc
     8581 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     8641 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
     8701 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
     8761 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
     8821 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
     8881 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc
     8941 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt
     9001 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt
     9061 ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca
     9121 caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt
     9181 ggaaggtgag tcgatgcgcg atgcaagtcg gcgatttctt tgaagttttg tcaaaagaag
     9241 agacaaggac ttgatagata cgctcatcag agtgagattc attgcaaata gtgtgtaaat
     9301 cgtggagaga cgagagaaaa ggtgtgagaa gcttagaaga taaagaatag gaagaagaac
     9361 atatctcatt tcctattttc tctaattttt cgttcatttt tctagacatt acccatttga
     9421 tggtttttca atatggttat cagggtactc attttggtag ttttcaaagc aatttggagt
     9481 ttttttttaa attgtcaact acaaaaacgc agcttaaagg tccaaatatc atagtataaa
     9541 aattctttaa aaaattccaa attttatatg attttttgaa aattggaaga atgttagttt
     9601 ttgccatttt tttggaagtt tttctccgga ttttatggta cttatatttc aaatataaat
     9661 ttttgtagga attcgagtat gcgagagagc tatagtttac taaaaaaaag tggcaaaaac
     9721 tcagtaattg ccactttttt caaaaatgtt ttgaaaaatt ttactatgat atttggccat
     9781 tttggcacca tatgtacgtt actttttacg aaacactcta atttttgaaa tttgagtttc
     9841 atgtttaatc ttttgtataa aatttttatg gtccttttgt gacaggataa aaaattgcaa
     9901 aaaaatagaa aactttgttt cgtgaaaaca cctagtttaa aaattgtggg ccacttagcg
     9961 attctagtca aaaatcagac aaatgtcagc tgccgtaact ttttccggat cagggttatc
    10021 aaattttaat tttaattgta aatgtagaaa aatgatcggc aacattttgc taattgaaaa
    10081 tattttgaaa attgctatag tacatattaa tctagtatta atatagtatt aatgttataa
    10141 tatcagttat gtgtttgaat aactcaagcg taagtttccg taaaatatct ttaaattttg
    10201 gtaatttttt acggtttggt ataaattttt gcattttttg attttctaca ctttttttcg
    10261 atttcgcatt ttccgaattt ctctgaaata aatcgaacaa tcggaaaatt ttcgagggca
    10321 cccttagcca tgcagctcag gaatttgact ttatgttccc attcaaattt ttttaaaatt
    10381 aaataaaatg ctaattgtaa gataaattac aaaaattatt aaatctcatc atccttatga
    10441 tgatagtgca gtgcatgaat atatttttcc aaataggata tgattcgtcg agtagcttca
    10501 cgtaatccgt tttcttcaat caccatttcg aactgcaatt ttttttttgg atttttgtaa
    10561 caatattgtc ataaattgtg attttgatat tttgcgtaga gcatcagaac ctttctgctt
    10621 tgaattttaa tgggattaaa aattataaaa aacttgcacc ctaattaaag cgcgatacct
    10681 ggtgacccat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10741 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10801 acattacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10861 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10921 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    10981 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11041 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11101 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11161 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11221 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11281 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    11341 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    11401 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    11461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    11521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttacgcg ccctgtagcg
    11581 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    11641 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gctttccccg
    11701 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    11761 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    11821 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    11881 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
    11941 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
    12001 attaacgttt acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctg
    12061 attatcaacc ggggtaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    12121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    12181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    12241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    12301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    12361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    12421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    12481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    12541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    12601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    12661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    12721 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    12781 attgggaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    12841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    12901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    12961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    13021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    13081 cgcacatttc cccgaaaagt gccacctgac gtagttaaca aaaaaaagcc cgccgaagcg
    13141 ggctttatta ccaagcgaag cgccattcgc cattcaggct gcgcaactgt tgggaagggc
    13201 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    13261 gattaagttg ggta
//
