LOCUS       sequence-204935-       17678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one genome editing vector in M. polymorpha (chlorsulfuron 
            resistant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17678)
  AUTHORS   Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, 
            Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
  TITLE     Efficient CRISPR/Cas9-based genome editing and its application to 
            conditional genetic analysis in Marchantia polymorpha.
  JOURNAL   PLoS One. 2018 Oct 31;13(10):e0205117. doi: 
            10.1371/journal.pone.0205117. eCollection 2018.
  PUBMED    30379827
REFERENCE   2  (bases 1 to 17678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-10-31"; volume: "13(10):e0205117. doi"; pages: " 
            10.1371/journal.pone.0205117. eCollection 2018"
COMMENT     Sequence Label: pMpGE014
FEATURES             Location/Qualifiers
     source          1..17678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2081
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2102..6205
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2102..6205
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6218..6238
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6741..6765)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    6794..6818
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        7379..7454
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(7496..7520)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7847..7863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7847..7863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7860..7882)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7871..7887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7895..7901,7902..7919,7920..7925))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7940..7961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    8046..8220
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(10330..11005)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    11200..11224
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11745..12536
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      12782..13370
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13271..13290
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13524..13541
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13556..13696
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13622..13641)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13782..13804
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14040..14234
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14300..15373)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15802..16431)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagcaggc tccgcggccg cccccttcac
     2101 catggataag aagtactcta tcggactcga tatcggaact aactctgtgg gatgggctgt
     2161 gatcaccgat gagtacaagg tgccatctaa gaagttcaag gttctcggaa acaccgatag
     2221 gcactctatc aagaaaaacc ttatcggtgc tctcctcttc gattctggtg aaactgctga
     2281 ggctaccaga ctcaagagaa ccgctagaag aaggtacacc agaagaaaga acaggatctg
     2341 ctacctccaa gagatcttct ctaacgagat ggctaaagtg gatgattcat tcttccacag
     2401 gctcgaagag tcattcctcg tggaagaaga taagaagcac gagaggcacc ctatcttcgg
     2461 aaacatcgtt gatgaggtgg cataccacga gaagtaccct actatctacc acctcagaaa
     2521 gaagctcgtt gattctactg ataaggctga tctcaggctc atctacctcg ctctcgctca
     2581 catgatcaag ttcagaggac acttcctcat cgagggtgat ctcaaccctg ataactctga
     2641 tgtggataag ttgttcatcc agctcgtgca gacctacaac cagcttttcg aagagaaccc
     2701 tatcaacgct tcaggtgtgg atgctaaggc tatcctctct gctaggctct ctaagtcaag
     2761 aaggcttgag aacctcattg ctcagctccc tggtgagaag aagaacggac ttttcggaaa
     2821 cttgatcgct ctctctctcg gactcacccc taacttcaag tctaacttcg atctcgctga
     2881 ggatgcaaag ctccagctct caaaggatac ctacgatgat gatctcgata acctcctcgc
     2941 tcagatcgga gatcagtacg ctgatttgtt cctcgctgct aagaacctct ctgatgctat
     3001 cctcctcagt gatatcctca gagtgaacac cgagatcacc aaggctccac tctcagcttc
     3061 tatgatcaag agatacgatg agcaccacca ggatctcaca cttctcaagg ctcttgttag
     3121 acagcagctc ccagagaagt acaaagagat tttcttcgat cagtctaaga acggatacgc
     3181 tggttacatc gatggtggtg catctcaaga agagttctac aagttcatca agcctatcct
     3241 cgagaagatg gatggaaccg aggaactcct cgtgaagctc aatagagagg atcttctcag
     3301 aaagcagagg accttcgata acggatctat ccctcatcag atccacctcg gagagttgca
     3361 cgctatcctt agaaggcaag aggatttcta cccattcctc aaggataaca gggaaaagat
     3421 tgagaagatt ctcaccttca gaatccctta ctacgtggga cctctcgcta gaggaaactc
     3481 aagattcgct tggatgacca gaaagtctga ggaaaccatc accccttgga acttcgaaga
     3541 ggtggtggat aagggtgcta gtgctcagtc tttcatcgag aggatgacca acttcgataa
     3601 gaaccttcca aacgagaagg tgctccctaa gcactctttg ctctacgagt acttcaccgt
     3661 gtacaacgag ttgaccaagg ttaagtacgt gaccgaggga atgaggaagc ctgctttttt
     3721 gtcaggtgag caaaagaagg ctatcgttga tctcttgttc aagaccaaca gaaaggtgac
     3781 cgtgaagcag ctcaaagagg attacttcaa gaaaatcgag tgcttcgatt cagttgagat
     3841 ttctggtgtt gaggataggt tcaacgcatc tctcggaacc taccacgatc tcctcaagat
     3901 cattaaggat aaggatttct tggataacga ggaaaacgag gatatcttgg aggatatcgt
     3961 tcttaccctc accctctttg aagatagaga gatgattgaa gaaaggctca agacctacgc
     4021 tcatctcttc gatgataagg tgatgaagca gttgaagaga agaagataca ctggttgggg
     4081 aaggctctca agaaagctca ttaacggaat cagggataag cagtctggaa agacaatcct
     4141 tgatttcctc aagtctgatg gattcgctaa cagaaacttc atgcagctca tccacgatga
     4201 ttctctcacc tttaaagagg atatccagaa ggctcaggtt tcaggacagg gtgatagtct
     4261 ccatgagcat atcgctaacc tcgctggatc tcctgcaatc aagaagggaa tcctccagac
     4321 tgtgaaggtt gtggatgagt tggtgaaggt gatgggaagg cataagcctg agaacatcgt
     4381 gatcgaaatg gctagagaga accagaccac tcagaaggga cagaagaact ctagggaaag
     4441 gatgaagagg atcgaggaag gtatcaaaga gcttggatct cagatcctca aagagcaccc
     4501 tgttgagaac actcagctcc agaatgagaa gctctacctc tactacctcc agaacggaag
     4561 ggatatgtat gtggatcaag agttggatat caacaggctc tctgattacg atgttgatca
     4621 tatcgtgcca cagtcattct tgaaggatga ttctatcgat aacaaggtgc tcaccaggtc
     4681 tgataagaac aggggtaaga gtgataacgt gccaagtgaa gaggttgtga agaaaatgaa
     4741 gaactattgg aggcagctcc tcaacgctaa gctcatcact cagagaaagt tcgataactt
     4801 gactaaggct gagaggggag gactctctga attggataag gcaggattca tcaagaggca
     4861 gcttgtggaa accaggcaga tcactaagca cgttgcacag atcctcgatt ctaggatgaa
     4921 caccaagtac gatgagaacg ataagttgat cagggaagtg aaggttatca ccctcaagtc
     4981 aaagctcgtg tctgatttca gaaaggattt ccaattctac aaggtgaggg aaatcaacaa
     5041 ctaccaccac gctcacgatg cttaccttaa cgctgttgtt ggaaccgctc tcatcaagaa
     5101 gtatcctaag ctcgagtcag agttcgtgta cggtgattac aaggtgtacg atgtgaggaa
     5161 gatgatcgct aagtctgagc aagagatcgg aaaggctacc gctaagtatt tcttctactc
     5221 taacatcatg aatttcttca agaccgagat taccctcgct aacggtgaga tcagaaagag
     5281 gccactcatc gagacaaacg gtgaaacagg tgagatcgtg tgggataagg gaagggattt
     5341 cgctaccgtt agaaaggtgc tctctatgcc acaggtgaac atcgttaaga aaaccgaggt
     5401 gcagaccggt ggattctcta aagagtctat cctccctaag aggaactctg ataagctcat
     5461 tgctaggaag aaggattggg accctaagaa atacggtggt ttcgattctc ctaccgtggc
     5521 ttactctgtt ctcgttgtgg ctaaggttga gaagggaaag agtaagaagc tcaagtctgt
     5581 taaggaactt ctcggaatca ctatcatgga aaggtcatct ttcgagaaga acccaatcga
     5641 tttcctcgag gctaagggat acaaagaggt taagaaggat ctcatcatca agctcccaaa
     5701 gtactcactc ttcgaactcg agaacggtag aaagaggatg ctcgcttctg ctggtgagct
     5761 tcaaaaggga aacgagcttg ctctcccatc taagtacgtt aactttcttt acctcgcttc
     5821 tcactacgag aagttgaagg gatctccaga agataacgag cagaagcaac ttttcgttga
     5881 gcagcacaag cactacttgg atgagatcat cgagcagatc tctgagttct ctaaaagggt
     5941 gatcctcgct gatgcaaacc tcgataaggt gttgtctgct tacaacaagc acagagataa
     6001 gcctatcagg gaacaggcag agaacatcat ccatctcttc acccttacca acctcggtgc
     6061 tcctgctgct ttcaagtact tcgatacaac catcgatagg aagagataca cctctaccaa
     6121 agaagtgctc gatgctaccc tcatccatca gtctatcact ggactctacg agactaggat
     6181 cgatctctca cagctcggtg gtgattcaag ggctgatcct aagaagaaga ggaaggtttg
     6241 aggcgcgccg agctccaggc ctcccagctt tcgtccgtat catcggtttc gacaacgttc
     6301 gtcaagttca atgcatcagt ttcattgccc acacaccaga atcctactaa gtttgagtat
     6361 tatggcattg gaaaagctgt tttcttctat catttgttct gcttgtaatt tactgtgttc
     6421 tttcagtttt tgttttcgga catcaaaatg caaatggatg gataagagtt aataaatgat
     6481 atggtccttt tgttcattct caaattatta ttatctgttg tttttacttt aatgggttga
     6541 atttaagtaa gaaaggaact aacagtgtga tattaaggtg caatgttaga catataaaac
     6601 agtctttcac ctctctttgg ttatgtcttg aattggtttg tttcttcact tatctgtgta
     6661 atcaagttta ctatgagtct atgatcaagt aattatgcaa tcaagttaag tacagtatag
     6721 gcttaagggt gggcgcgccg acccagcttt cttgtacaaa gtggttgata acagcggttg
     6781 actagagtta tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttgtaacg
     6841 tgagactact acactcctca acgcaagttc tatctcatat ctctgcaaga gcttattcca
     6901 agaaactttc ttgtgcttct atgcatgccc aggtacctta agtcgcgcgc atagtcactt
     6961 cacagccttt tcagttctcc ctttgatggc tcagagtgac gcaactgcac aaaaataaga
     7021 aaaaagtcga agcgtcacga gagatgtatc cggctagttt ccttggagtt cggattgctc
     7081 tctttctctg tctccccgaa ccatatggaa tttatgaact gctgttcact agtcagtcac
     7141 ttgtttcaga acttttgagt ggtcgattta tccaccttct tgtttcttcc tgggtccaga
     7201 atctgacttc ttgagacaac aaacagtgca cagtggatcg aagaatgacc acagttgaga
     7261 cacgtggcgc tggcctcatt tattgcacca tacgtttagt gaagaactga ataatggtgg
     7321 atataaagca gagttgcacc cagcctctcg aaatgcaggt gatgactcac ctgcataagt
     7381 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     7441 caccgagtcg gtgctttttt tctagtatcg ggaattctaa agggtgggcg cgccgaccca
     7501 gctttcttgt acaaagtggt tgataacagc gcttagaggc ttagagctcg aatttccccg
     7561 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     7621 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     7681 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     7741 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     7801 tgttactaga atcgggaaat ggttccggaa ccaattcgta atcatggtca tagctgtttc
     7861 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7921 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt aggcgcgcct
     7981 atttctgaat tcaacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     8041 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     8101 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     8161 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     8221 ctcgagttag tatatagtcc ttccatcacc ctctgtgata atctccttaa aacttgctcc
     8281 cccaggaatc ataggcagaa cgtgctcctg gtggggaact atcacatcca gtaaataagg
     8341 accgggagaa tctagcatag tttgaattgc tgctcttagt tcatccttct tcaccacacg
     8401 cgcatttggc accttgaaac cgtctgcaac tgtacagaag tcggggtaga tctgctcctc
     8461 gtgatctgga tcacctaagt aagtatgtgc tctgttggcc ttgtagaatc tatcctccaa
     8521 ttgtacgacc attcctagat gttggttgtt taagatcatg atcttcacgg gtaacttctc
     8581 cacaaaaatt gtagccaact cctgtacgtt catcacaaag ctaccatcgc catcgatgtc
     8641 gatcacagga atatctggtt gagttgcggc agcccccaaa gcagaaggaa gtccgaatcc
     8701 catggctcca agacctccag atgtcaacca acgccgcggt tcgttgtact tgaaccactg
     8761 ggctgcccac atttgatgtt ggccaacacc agtagatatg atggcatttc caccagtgag
     8821 ttcgtacagc gtctgtatgg cagattgagg agcaatggca tcctcgaata cgggaaactt
     8881 cattggccat ttctgctttt gttcttctag ttctgccacc cagttggaga agtcgaaagt
     8941 cctcttttga ctcttcaaga gggcattgat ttcacttagc gcaggtttca catcagcaca
     9001 catggacaca tggggctgtt tgttctttcc aatttcagca gggtcaatat cgatatgaac
     9061 gatgctggac ctgctagcaa aagtctccag tttaccagtc actcgatcgt cgaaacgcac
     9121 tccaaatgcc aacaacagat ctgcttggtc cacggcgtag ttggcgtaaa cagttccatg
     9181 cattccaagc attttcaaac ttaatggatc agactcggga tacgctccca aacccattaa
     9241 agtcgatgtg ataggaattc cggtaagctc gacaaattcc ctcaactctt cgctggcgtg
     9301 attgcaacct cctccagaat acactacagg cttcttagaa ctagaaatga gtcttataat
     9361 ctgctcgatt aacgttggtt ccggtggccg aggcagtcgt tgtaagtagc ccttcagttg
     9421 cattggctga gaccaatctg gaatagccat ttgctgttgc acgtccttgg gaatatctac
     9481 caagactggg cccggcctac ctgaagctgc caaaaagaaa gcctccctga ttacgcgagg
     9541 gatgtcatca atgttcataa caaggtaatt gtgcttggtg atcgatctcg taacctccac
     9601 aattggagtt tcttgaaaag catcggtacc aatcatccta ctggatactt gacctgtaat
     9661 ggccactagt ggcacactgt ccaagagggc atcagcgagc ccggtaacaa gattggtcgc
     9721 tccaggaccg gatgtggcta tacaaacccc tactcgtcca gacgatttag cataaccttc
     9781 agcagcgaaa atctccccct gctcgtggcg acagagaatg ttgcggatgc tgctggaacg
     9841 tgtaagagcc tggtgaatct ccatagacgc tccgccggga taagcgaaca ccgttctaat
     9901 cccttctcgc tcaagagcct ccaccaaaat gtcggctcct ttgcggggct cgtcatcctt
     9961 gtagcgagac tgaaagtcgg acggggttgg tgcaacgagt gacttcccgc tgctaagccg
    10021 tggtccactc acatcgactg cagactggat cttctcgcta acagcttggg ctttcacttc
    10081 cagtgaacgc tcagtcttcc gagaggtgtt gtatgcatgt cccctcagag cggctcccga
    10141 gaagaaacca ctcctcacct ttagctcttt agctcccttg caacaagacc ttgccgatgg
    10201 ctgcaccttc tccaggctcg ctatgctcaa aggcacagct gcgcatccac taattgacgc
    10261 catatctcat tgccccccgg atctgcgaaa gctcgagaga gatagatttg tagagagaga
    10321 ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaaggtct
    10381 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
    10441 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
    10501 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
    10561 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
    10621 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
    10681 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
    10741 tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
    10801 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
    10861 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
    10921 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
    10981 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
    11041 attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata
    11101 cgcaaaccga attcagaaat aaattcagcc taattcggcg ttaattcagt acattaaaaa
    11161 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
    11221 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
    11281 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
    11341 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
    11401 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
    11461 caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
    11521 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
    11581 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
    11641 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
    11701 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
    11761 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
    11821 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
    11881 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
    11941 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
    12001 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
    12061 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
    12121 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
    12181 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
    12241 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
    12301 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
    12361 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
    12421 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
    12481 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
    12541 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
    12601 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
    12661 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc
    12721 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    12781 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    12841 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    12901 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    12961 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    13021 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    13081 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    13141 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    13201 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    13261 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    13321 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    13381 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    13441 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    13501 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
    13561 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
    13621 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
    13681 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
    13741 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
    13801 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
    13861 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
    13921 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
    13981 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
    14041 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
    14101 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
    14161 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
    14221 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
    14281 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
    14341 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
    14401 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
    14461 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
    14521 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
    14581 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
    14641 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
    14701 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
    14761 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
    14821 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
    14881 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
    14941 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
    15001 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
    15061 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
    15121 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
    15181 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
    15241 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
    15301 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
    15361 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
    15421 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
    15481 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
    15541 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
    15601 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
    15661 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
    15721 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
    15781 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
    15841 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
    15901 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
    15961 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
    16021 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
    16081 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    16141 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    16201 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    16261 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    16321 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    16381 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    16441 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    16501 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    16561 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    16621 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    16681 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    16741 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    16801 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    16861 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    16921 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    16981 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    17041 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    17101 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    17161 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    17221 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    17281 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    17341 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    17401 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    17461 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    17521 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    17581 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
    17641 gttggcatgc acatacaaat ggacgaacgg ataaacct
//
