LOCUS       sequence-204952-       10296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS
            specifically with Venus and DsRed lazers. Tet-ON-all-in-one.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10296)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 10296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pLT3REVIR_MARK3 2573
FEATURES             Location/Qualifiers
     source          1..10296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..126
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    619..852
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1037..1081
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1348..1465
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1483..1861
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1491..1509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1527..1545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1563..1581
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1599..1617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1635..1653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1671..1689
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1707..1725
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1770..1794
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1885..2562
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2065..2085)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2477..2500
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           2746..2760
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        2939..3438
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2974..2997)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3371..3390
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(3461..3463,3464..3466,3467..4180)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3506..3527)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3767..3786)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4114..4135
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4193..4760
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4210..4762
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4354..4371)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4581..4600
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4774..5391,5392..5397,5398..5433,5434..5436,5437..5472,
                     5473..5475,5476..5511,5512..5520)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5566..6154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5619..5639)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6037..6048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6361..6594
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6666..6787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6703..6722)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6757..6776
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6827..6962
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6982..7001)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6983..7001)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7011..7028)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7011..7027)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7020..7042)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7169..7624
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7256..7275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7466..7487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7650..7754
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7755..7823,7824..8615)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7973..7992)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8786..9374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9275..9294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9528..9545
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9662..9683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9698..9703,9704..9721,9722..9728)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9736..9752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9741..9763
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9760..9776
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9760..9776
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9795..9815
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9797..9815
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9843..10069
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10070..10250
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
       61 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      121 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
      181 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
      241 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
      301 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
      361 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
      421 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
      481 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
      541 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
      601 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
      661 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
      721 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
      781 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
      841 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
      901 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
      961 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1021 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1081 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     1141 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     1201 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     1261 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     1321 gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag
     1381 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     1441 acaaattaca aaaattcaaa attttatcga tcctctagat tcgagtttac tccctatcag
     1501 tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt
     1561 actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg
     1621 tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag
     1681 tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttag
     1741 gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca gatcgcctgg
     1801 agcaattcca caacactttt gtcttatacc aactttccgt accacttcct accctcgtaa
     1861 agtcgagctt gcgttggatc caccatggcc tcctccgaga acgtcatcac cgagttcatg
     1921 cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg agttcgagat cgagggcgag
     1981 ggcgagggcc gcccctacga gggccacaac accgtgaagc tgaaggtgac caagggcggc
     2041 cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac
     2101 gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag
     2161 tgggagcgcg tgatgaactt cgaggacggc ggcgtggcga ccgtgaccca ggactcctcc
     2221 ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt cccctccgac
     2281 ggccccgtga tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     2341 cgcgacggcg tgctgaaggg cgagacccac aaggccctga agctgaagga cggcggccac
     2401 tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct gcccggctac
     2461 tactacgtgg acgccaagct ggacatcacc tcccacaacg aggactacac catcgtggag
     2521 cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt agcggccgcc tcgactaggg
     2581 ataacagggt aattgtttga atgaggcttc agtactttac agaatcgttg cctgcacatc
     2641 ttggaaacac ttgctgggat tacttcgact tcttaaccca acagaaggct cgagaaggta
     2701 tattgctgtt gacagtgagc gaagtgatta tgatgtaaat taatagtgaa gccacagatg
     2761 tattaattta catcataatc actgtgccta ctgcctcgga cttcaagggg ctagaattcg
     2821 agcaattatc ttgtttacta aaactgaata ccttgctatc tctttgatac atttttacaa
     2881 agctgaatta aaatggtata aattaaatca cttttttcaa ttgacgcgta attctaccgg
     2941 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     3001 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     3061 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     3121 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     3181 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     3241 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     3301 gggtccgggg gnnggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     3361 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     3421 tcatctccgg gcctttcgac ctgcagccca agcttccacc atggtgagca agggcgagga
     3481 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3541 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct
     3601 gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta
     3661 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3721 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3781 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3841 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     3901 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
     3961 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     4021 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     4081 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4141 cgccgggatc actctcggca tggacgagct gtacaagtaa gaattgttca attccgcccc
     4201 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     4261 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     4321 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     4381 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     4441 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     4501 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     4561 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     4621 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     4681 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     4741 tttgaaaaac acgatgataa tatggccaca accatgagta gactggacaa gagcaaagtc
     4801 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     4861 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     4921 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     4981 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     5041 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     5101 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     5161 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     5221 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     5281 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     5341 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     5401 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     5461 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     5521 tccggactcg acctgcagcc aagcttatcg aaacgcgtct ggaacaatca acctctggat
     5581 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5641 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5701 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5761 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5821 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5881 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5941 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     6001 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6061 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6121 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     6181 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     6241 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     6301 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6361 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     6421 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6481 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6541 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6601 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6661 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6721 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6781 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     6841 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6901 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6961 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7021 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7081 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7141 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7201 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7261 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7321 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7381 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7441 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7501 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7561 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     7621 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7681 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7741 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7801 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7861 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7921 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7981 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8041 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8101 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8161 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8221 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8281 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8341 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8401 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8461 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8521 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8581 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8641 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8701 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8761 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8821 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8881 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8941 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9001 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9061 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9121 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9181 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9241 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9301 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9361 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9421 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9481 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9541 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9601 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9661 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9721 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9781 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     9841 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     9901 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     9961 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
    10021 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
    10081 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10141 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10201 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    10261 aacagggact tgaaagcgaa agggaaacca gaggag
//
