LOCUS       sequence-20497-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..152)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(160..176)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(160..176)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(191..632)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(197..220)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    515..632
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(656..676)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(656..674)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcctgggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt tttacagtat
       61 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      121 gtttctcgtt cagctttttt gtacaaactt gtgatggggg atccactagt tctagaatcc
      181 ggggtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact
      241 tttattacat ttgaataaga agtaatacaa accgaaaatg ttgaaagtat tagttaaagt
      301 ggttatgcag tttttgcatt tatatatctg ttaatagatc aaaaatcatc gcttcgctga
      361 ttaattaccc cagaaataag gctaaaaaac taatcgcatt atcatcctat ggttgttaat
      421 ttgattcgtt catttgaagg tttgtggggc caggttactg ccaatttttc ctcttcataa
      481 ccataaaagc tagtattgta gaatctttat tgttcggagc agtgcggcgc gaggcacatc
      541 tgcgtttcag gaacgcgacc ggtgaagacg aggacgcacg gaggagagtc ttccttcgga
      601 gggctgtcac ccgctcggcg gcttctaatc cgtactagag ctcccgcttt tgttcccttt
      661 agtgagggtt aattgcgcgc ttggc
//
