LOCUS       sequence-204975-        2107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 
            1557-1617 replaced by SGGSG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Park S, Stanfield RL, Martinez-Yamout MA, Dyson HJ, Wilson IA, 
            Wright PE
  TITLE     Role of the CBP catalytic core in intramolecular SUMOylation and 
            control of histone H3 acetylation.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114.
  PUBMED    28630323
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114."
COMMENT     Sequence Label: His6tev CBP BCHZDeltaAL
FEATURES             Location/Qualifiers
     source          1..2107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             28..50
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             109..129
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      1606..1615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2051..2068
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggaccgcaca ttcccctcta gaataatttt gtttaacttt aagaaggaga tatacatatg
       61 tcgtactacc atcaccatca ccatcacgat tacgatatcc caacgaccga aaacctgtat
      121 tttcagggcg ccatgggatc ctcccagcca cgcaaaaaaa tctttaaacc cgaggagcta
      181 cgccaggcac ttatgccaac tctagaagca ctctatcgac aggacccaga gtctttgcct
      241 tttcgtcagc ctgtagatcc tcagctccta ggaatcccag attattttga tatagtgaag
      301 aatcctatgg acctttctac catcaaacga aagctggaca cagggcaata tcaagaaccc
      361 tggcagtatg tggatgatgt ctggcttatg ttcaacaatg cgtggctata taatcgtaaa
      421 acgtcccgtg tatataaatt ttgcagtaaa cttgcagagg tctttgaaca agaaattgac
      481 cctgtcatgc agtctcttgg atattgctgt ggacgaaagt atgagttctc cccacagact
      541 ttgtgctgtt acggaaagca gctgtgtaca attcctcgtg atgcagccta ctacagctat
      601 cagaataggt atcatttctg tgagaagtgt ttcacagaga tccagggcga gaatgtgacc
      661 ctgggtgacg acccttccca acctcagacg acaatttcca aggatcaatt tgaaaagaag
      721 aaaaatgata ccttagatcc tgaacctttt gttgactgca aagagtgtgg ccggaagatg
      781 catcagattt gtgttctaca ctatgacatc atttggcctt caggttttgt gtgtgacaac
      841 tgtttgaaga aaactggcag acctcggaaa gaaaacaaat tcagtgctaa gaggctgcag
      901 accacacgat tgggaaacca cttagaagac agagtgaata agtttttgcg gcgccagaat
      961 caccctgaag ctggggaggt ttttgtcaga gtggtggcca gctcagacaa gactgtggag
     1021 gtcaagccgg gaatgaagtc aaggtttgtg gattctggag agatgtcgga atctttccca
     1081 tatcgtacca aagcactctt tgcttttgag gagatcgatg gagtcgatgt gtgctttttt
     1141 gggatgcatg tgcaagaata cggctctgat tgccccccac caaatacaag gcgtgtatac
     1201 atatcttatc tggacagtat tcatttcttc cggccccgct gcctccggac agctgtttac
     1261 catgagatcc tcatcggata tctcgagtat gtgaagaaat tggggtatgt gacaggacat
     1321 atttgggcct gtcccccaag tgaaggagat gactatatct ttcattgcca cccccctgac
     1381 cagaaaatcc ccaaaccaaa acgactacag gagtggtaca agaagatgct ggacaaggcg
     1441 tttgcagaga ggatcattaa cgactataag gacatcttca aacaagcgaa cgaagacagg
     1501 ctcacgagtg ccaaggagtt gccctatttt gaaggagatt tctggcctaa tgtgttggaa
     1561 gaaagcatta aggaatctgg aggatcagga tcgcagaagc tgtatgccac catggagaag
     1621 cacaaggagg tattctttgt gattcatctg catgctgggc ctgttatcag cactcagccc
     1681 cccatcgtgg accctgatcc tctgcttagc tgtgacctca tggatgggcg agatgccttc
     1741 ctcaccctgg ccagagacaa gcactgggaa ttctcttcct tacgccgctc caaatggtcc
     1801 actctgtgca tgctggtgga gctgcacaca cagggccagg accgctttgt ttatacctgc
     1861 aatgagtgca aacaccatgt ggaaacacgc tggcactgca ctgtgtgtga ggactatgac
     1921 ctttgtatca attgctacaa cacaaagagc cacacccata agatggtgaa gtgggggcta
     1981 ggcctagatt aataggtcga cgagctcact agtcgcggcc gctttcgaat ctagagcctg
     2041 cagtctcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagca
     2101 gcgatat
//
