LOCUS       sequence-204981-       14708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Loureiro RM, Maharaj AS, Dankort D, Muller WJ, D'Amore PA
  TITLE     ErbB2 overexpression in mammary cells upregulates VEGF through the 
            core promoter.
  JOURNAL   Biochem Biophys Res Commun. 2005 Jan 14. 326(2):455-65.
  PUBMED    15582599
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2005 Jan 14. 326(2):455-65."
COMMENT     Sequence Label: 9 kB VEGF-luc
FEATURES             Location/Qualifiers
     source          1..14708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8892..10544
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(8958..8976)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          10785..10850
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10980..11000
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11425..11559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11462..11481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11516..11535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11600..11620)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11600..11618)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11639..11655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11639..11655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11652..11674)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11663..11679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11687..11693,11694..11711,11712..11717))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11732..11753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11870..11887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12041..12629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12121..12140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12800..13591,13592..13660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13423..13442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13661..13765)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13791..14246)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13928..13949)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14140..14159
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14373..14395
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14387..14404
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14388..14404
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14414..14433
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14414..14432
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagcccagat gggcctcaaa ctcgccatgt agccaaagat gatgctagat tctcaatcct
       61 cctgcctctt ccttccaagt gagagaatta aatctaccca caagtgctgc gattgcaggc
      121 gtccaccatc acacttggtt ttatgtgctg ctggggaatg aacccagggc ttcacacatg
      181 ccgagcaagc taactcacta agctctctgc tcagccccaa agcttggttt tgagtccagg
      241 tgacttcagg gtgagaccca gactttggct tcctaggcag cagcagtgca aagctagagc
      301 tttagaaacc ccactaaccc acagggttct taaggctata cccagggtag tatcaagagg
      361 tgatgctgcc cttccttccg ctgcaggacg cagtgaccca ggtcattttg cctggaccag
      421 ctgggtttct gagaacactg ttgtctcttc tatgggacca tgttgtgggt agtggtggtg
      481 gtggtggggt ttgtttgtgt atgtgtatat atatatgtgt ggtttattag ttctgtggcc
      541 tttggtgtgg tcagttgagg gacataaagg ggcagaagat tggtgggtgg gggtaagctg
      601 aggtggagtg gttccttggg cttccatcag cagtgggtgg aaggcaatgt gttcacctct
      661 gtccccttag agcctcaggc cacaccaggg ccttagagga ggagcagctt tgagccttca
      721 ctctctagag gtgcaatgct gttttcccca gctaagcagg agtatagcct gttccctcag
      781 aagcatccca caagactcct cagatcccaa gggatcatga gaattggggg tatgaagacc
      841 atcttttcta gattgtcccc tgcttcccag tgtgtacccc ccaaaagaaa tgtgaaacag
      901 ggagaagtca gagcctctta gaggctgaag cttgtgacag tctcagaaag gcagttagcc
      961 aacagaattg tcccagaagg attcctggga caaatgacca ggccaagaag agggacagag
     1021 acactccagg aaagagagag gaagaagaga tagaaaactg tcctggttac tatagctgtc
     1081 cacgacatag gcctgtcacc acttgggctc gaatttcaat cctgacccag ctggtcatgt
     1141 ttgcaacctg gccaggaacc tgacagttca caaccccatt cctctagagg gtgctggaga
     1201 tcaaagatgg agttcctgcc ccagggacat aggcaaagtg tgtgtgtgtg tgtgcccaca
     1261 cacacacccc tgggatggca cagggtttgg atggtaacct gctgttttgc acgttggcca
     1321 cacttgcttc tggaaattaa gtgcaagtcc tagcctggtc cttctaagag ctgatgaaac
     1381 ctgggaccat gtagacctgg acccaaggtg ggcctcagac agatcctggg cagagactgc
     1441 ctatgcattc tacctctgag cagagagtgg tccaaggcag aatgagaatc cagctgacat
     1501 gcaccagcaa cagcttcctg attggcaaaa agctgccttt gagtccccca ctcctagaca
     1561 catggctttc tggctaccct tattcctctt ctaagtttcc tgggctctcc tgcactctag
     1621 ataccagaga gctatgcttg gcacagcaga tgcccccagt gccctgtgtc ccctgcctga
     1681 gacacagcag gagctgactg ctttagccct cttctcagat ggtgaggaca gtatcccctc
     1741 cccattaggg actaagtgtg ccctgccttg cctcttttga tgcttttagc tgtgttctgg
     1801 gagggcatgg gtcctagctg atgtgaaggg agatgtagat aggactgatt gacgagggaa
     1861 cccaaatggg gttgggtgta gctcattctt gatgctctga ggagggatgc ctctgggaag
     1921 cagggagaag cccggctgtt aaactgctct gatgcacacc ggcagccctg ggctggcact
     1981 gcccagctct ctggccaatc agtcagctct tgtggccaga agccccacgt atcccaaagt
     2041 cacccatctt caaaatgatc tggtggcttt tcgagaggca gagagaaaga tcggagttta
     2101 ggtttgcctt ctgtttggct tcagccagct ctgggcccca cttggccaga attgtgattt
     2161 tttttcaaac ttctttagta atgagtcgtt tcttcatgtg acattccaaa gaaaacatca
     2221 ataaaggaaa actgggagct gggtctgccc acgctaaccc ctaccgctta cctccgagga
     2281 ccccaagagt ctcagagcaa gtttgatgtt ctcctggctc agataacctt tgatgaattg
     2341 cttcaccggt tatctagtcg gaactaggcc aggaaggttc tcgaagctaa gggaggtctc
     2401 cctgttccct gacaccctac tctccttgca cttctgggtg tgggaatgag ggcaggatag
     2461 tgggatagcc ccctctggga agcaatcatt aactggtgtt atctttgtga agtgtcttac
     2521 agggcaggcc atgagccagg ttttcaccta cagtcctggt gggttctggg ggacggggcc
     2581 cttgctggca gggcctgact ctttttctaa cttccctgaa ggaatgcatt gcagccacct
     2641 ttgtgaccat tggtgagagt tgcttcacgg aaaggcaaaa gcagaaacga agctaacaca
     2701 cccagcagct ctgtcatctc cagtgtacat tatgggattg atttcttcct tcatctctcc
     2761 ctccctccct ccctcagctc ttcattcctg gcttccttcc tccccttctc tttcttcctt
     2821 tattgttttt ctgtgacagg gcctcgtgta acttaggctg gccttgaaag caaaacacta
     2881 tattgatagg atgaccttga actctatcca gttctttctt cctctacctt tcaaattctg
     2941 gggttacaag catgtggtcc cttcccatcc ccttcccttc tttcacctag gtgtggagta
     3001 tatctcttct cccagccctc cctgactcta gccagatcat attggcttgg gaaatgaacc
     3061 agataagcac acgtacatca actcttacac ctctctacaa ccctggcagg aggcaacaac
     3121 actttccatt tcacagatga gaagactgag gctcacagct ccggaaatag tgaaccagat
     3181 ttgtgctccg tctgccatgt tttcccattc ctcggtatgg gttgggtggg ggtgtggggt
     3241 gtagcagaag ccaaggctct tcacagtgag ggatcaccaa ccagtgactt caaggaagat
     3301 gaggctagtt ggttatgcgt atgactaatt ctagtaatcc cactacttga aaatttgagg
     3361 caggaggatt atgaattcca agccagcctg ggatacatag taagaccaaa agatgaagat
     3421 aaaacagaaa caagctgaaa ctaaaatgac tgaggagggg ttagaagagg gagttggagt
     3481 ttcctgtctt ctgggctgaa ggtgtgtctc gcctccatta accggtgcta ggccccggat
     3541 gtcaccatgg cattcctgct agattttttt ggtgttaaac attgctctct tcaagagtca
     3601 taaggacaca ggccacccga agacgtgaca tttggtatca aggaacagtc tcagctgtca
     3661 ctgtctctag tgactcagag ctagggaatc ttgcctagaa gtggagggcc agcagcagga
     3721 ctggtgaact ggtgctgacc aaggtcacac tgcctgtggc cttctcagct ctcctggagg
     3781 cagggacaga ctagagaaag tgttatcctg ctttgtgctc tggtctagcc aactggccct
     3841 gggcttatcc caggttgctg cttagggcaa acgatggccc cagcactcag tatatgacta
     3901 gacctacctc tactgtctgg atggtttttt ttaaaacact ctctggggac gtattacttc
     3961 cttcctggta ggggactgcc aagaaagcag cgtggaactt acccctgaca gaacagtatt
     4021 gtcccctggg ctctgtctag gatgttcctt aaaacaatcc ctcagctggg cctaccctct
     4081 aagtctttat cctcagaccc agaagaggac ctgggaggca gccttacaga aatctctaga
     4141 aatcccttgt ctcttctccc cagttcaccc caatggctaa attcctgaaa ctcctgcttc
     4201 tcccccaaaa cccaactgaa cgtgtcaggt ccttcaggat ggcgtcacct gctcctagca
     4261 gttatgtagc ccatcacttt agccttttgc tcacctgcag acgggaagtc tcttccacac
     4321 tgtgggaaaa cagggtcctg cccaaatcag agcttgcccg aggaatgtgg caaccgctag
     4381 ggagtctgtg ccccatgcca cttcatgcag gaagcatttt gggccagcca atatggctgc
     4441 tttgctggct aatgctgaaa gacttttccc ccccacattg cctttggtat actattaaaa
     4501 gataagaaaa atgacgagaa ggaagaggaa gaggaggaag aggaggaaga agaagatgag
     4561 gaggaggagg aggaggagga aagatcgatt tttttggcct ttactttgaa agggctgaca
     4621 tggctgcccc acgcccctgg tttgagaaag gtttcaacgt gaagcctagg ttctgcttat
     4681 tttatgaatc tcctgagtgt tggggattcc agatgagcat tcccatgccc aacctgactt
     4741 tatttcagtt ctgacaatct cctccctgtc tctgaacaag attctctgaa gatgtcactg
     4801 actaaccaac cacctagtgg attgctccct agagccccat gatgccagga aaagattcat
     4861 cctggttggt ctgacttgcg ttagccagca tgagatcgac agggggaacc cccatttctt
     4921 tgggggtaca ctgtttaaat aggttgagag acacagggat ctgtttagaa tatttcatgt
     4981 ctgccaggcc cactacactc ccatgtctcc tgtcctgcag tgacctgggg gagagacgct
     5041 gaatagaatc tattctggga aatgagcccc caggaagagc aggaatagtg ggatacttgt
     5101 cacttcccct cttctttcta gattccctga ggtttcatta agtgacactt actaggtcca
     5161 tatcagtgta cggatgacac cattgtggaa tgtgttggag gcaatgtgca gcttgtatgg
     5221 aatccagaag tgacccttcc tctgacccag cttcagattg tgtctctaaa atgcatgacg
     5281 gaggtagttg gcccatccct accctgatcc gggttactcc aaacccttca ggccaagttt
     5341 cagtctttgc atggctttag tgctttggag agggaaatgt gcagcgtcac tgctctggct
     5401 ccctgtacag gctcatctgg gaacacctta tacatatata tacacatata ctacatgtat
     5461 atatacatat actatacata tacatatcat ggctatctca ggataggagg aggagtctaa
     5521 agccgtgaag tcgagaagag ctgattcccc tcaaagtctc ttcattttgc tccaaacctc
     5581 agaatccttt actgagtccc tcttctgtag gcccgtggcc tttcccaaga ccacagaact
     5641 gtggttctca gccttcctaa tgctgagacc ctttagtaca gttcctcatg ttgtggtgac
     5701 ccccccagcc cccccccaca ggcaacataa aaatattttt gttgctactt cataactgta
     5761 attttgttac tattatggat cataatgtaa ctctctgaca tgcaggccat ctgatatgcg
     5821 acccctgtga aagggttgtt ggacagtccc tccaaagggt tgagggttga gaatcacagc
     5881 tggaggaggt ggagatgaga agggcagcgc tttgggagcc ttcagtgtct acagcctctg
     5941 ttatgccacg atgagagaca ggggccccct gacagggagc tacatttggt gttcttgcgg
     6001 ttagagacag acacgtgaga catttggtgg ctttcctttc tgactcctct tagttgctta
     6061 gagcagggat caggatcagg tgaggaaacc cacaggaggg ctctggacca gcaaacattt
     6121 actaactact actttccctg ctacaagaga gccatccagg aggggactcg ggactagtaa
     6181 tgatgggcag gaagggatag cgtgaggagc cagctccctt cctcataaga tcctcatact
     6241 actgctccat tggaattggg gtgcccaaga ggtgccagca cccacccagc ttcagtactg
     6301 tgtgcagagg gatggagata gtggattaca ctgggggggg ggggcaatag gaagattgtt
     6361 gagttctctt tctcagaagt ccagcagatc tgggtgaggg ctgggactgg ttggtccctc
     6421 tcttcccaca ggtatcggag ccccctcttg ttcccagggg agcctaggag cagctgggcc
     6481 aaagcccaac caggaatttt tccaggctgg ttcctatatc caagggtggg ttagaggtgg
     6541 gggttttgga gagctcttaa ggaagacact gaggacatgg agaaggggac ttagtagaaa
     6601 gagaggggac agagccacac gggctaagtg tgcaagcata gagaaatagc caagggtttt
     6661 agggagaatg ggaggacaga gtacacccct gaattctgtt tagaagatga accgtaagcc
     6721 taggctagaa ctgagggagc ctctactccc acccttccga gggttggcgg cagcgctggg
     6781 cagctggcct acctaccttt ctgaatgcta gggtaggttt gaatcaccat gccggcctgg
     6841 cccgcttctg cccccattgg caccctggct tcagttccct ggcaacatct ctgtgtgtgt
     6901 gtgtgtgtgt gtgagagaga gagatcagga ggaacaaggg cctctgtctg cccagctgtc
     6961 tctccttcag ggctctgcca gactacacag tgcatacgtg ggtttccaca ggtcgtctca
     7021 ctccccgcca ctgactaact ccagaactcc acttcccgtt ctcagtgcca caaatttggt
     7081 gccaaattct ctccagagaa gcctctctgg aaacttccca gaggatccat tcaccccagg
     7141 gccctagctc ctgatgactg cagatcagac aagggctcag ataagcatac tccccccccc
     7201 ccgtaacccc ctccccacat ataaacctag agttatgctt ccgaggtcaa acacgcaact
     7261 ttttgggtgt gtgtgtatgt cagaaacacg caattatttg ggagctcaaa gtctgccgca
     7321 ctcaagaatc aactctcacc ccctttccaa gacccgtgcc atttgagcaa gagttggggt
     7381 gtgcataatg tagtcactag ggggcgctcg gccatcacgg ggagatcgtg aacttgggcg
     7441 agccgagtct gcgtgaggga ggacgcgtgt gtcaatgtga gtgcgtgcat gcatgtgtgt
     7501 gtgtgtgtag tgtgtttgtg aggtggggga gaaagccagg ggtcactcta gttgtcccta
     7561 tcctcatacg tccctgccag ctctccgcct tccaacccct actttctcct atattcctgg
     7621 gaaagggaat tgttcttaga ccctgtccgc atataacctc actctcctgt ctcccctgat
     7681 tcccaatact ctgggattcc cagtgtgttc ctgagcccag tttgaagggg tgcacagata
     7741 attttgaggc cgtggaccct ggtaaggggt ttagctttcc atttcgcggt agtggcctag
     7801 gggctccccg aaaggcggtg cctggctcca ccagaccgct ccccggggcg ggtctgggcg
     7861 gggcttgggg gtggagctag atttcctctt tttcttccac cgctgttacc ggtgagaagc
     7921 gcagaggctt ggggcagccg agctgcagcg aggccgcggc actgggggcg agctgagcgg
     7981 cggcagcgga gctctgtcgc gagacgcagc gacaaggcag actattcagc ggactcacca
     8041 gcccgggagt ctgtgctctg ggatttgata ttcaaacctc ttaatttttt tttcttaaac
     8101 tgtattgttt tacgctttaa tttatttttg cttcctattc ccctcttaaa tcgtgccaac
     8161 ggtttgagga ggttggttct tcactccctc aaatcacttc ggattgtgga aatcagcaga
     8221 cgaaagaggt atcaagagct ccagagagaa gtcaaggaag agagagagag accggtcaga
     8281 gagagcgcgc tggcgagcga acagagagag ggacaggggc aaagtgactg acctgctttt
     8341 gggggtgacc gccagagcgc ggcgtgagcc ctcccccttg ggatcttgca tcggaccagt
     8401 cgcgctgacg gacagacaga cagacaccgc ccccagcccc agcgcccacc tcctcgccgg
     8461 cgggctgccg acggtggacg cggcggcgag ccgcgaggaa ccgaagcccg cgcccggagg
     8521 cggggtggag ggggtcgggg ctcgcgggat tgcacggaaa cttttcgtcc aacttctggg
     8581 ctcttctcgc tccgtagtag ccgtggtctg cgccgcagga gacaaaccga tcggagctgg
     8641 gagaagtgct agctcgggcc tggagaagcc ggggcccgag aagagagggg aggaagagaa
     8701 ggaagaggag agggggccgc agtgggcgct cggctctcag gagccgagct catggacggg
     8761 tgaggcggcc gtgtgcgcag acagtgctcc agccgcgcgc gcgccccagg ccccggcccg
     8821 ggaggtaccg agctcttacg cgtgctagct cgagatctaa gtaagcttgg cattccggta
     8881 ctgttggtaa aatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc
     8941 tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
     9001 ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact
     9061 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
     9121 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
     9181 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
     9241 gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt
     9301 tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg
     9361 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
     9421 atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga
     9481 attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg
     9541 tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
     9601 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
     9661 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ttacgatccc
     9721 ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca ttcttcgcca
     9781 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctgggggcg
     9841 cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt ccagggatac
     9901 gacaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
     9961 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
    10021 ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga ggacctatga
    10081 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
    10141 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atagttgacc
    10201 gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa ttggaatcga
    10261 tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg
    10321 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
    10381 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
    10441 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
    10501 agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaaatgta actgtattca
    10561 gcgatgacga aattcttagc tattgtaata ctgcgatgag tggcagggcg gggcgtaatt
    10621 tttttaaggc agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa
    10681 taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac ttctgtggtg
    10741 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
    10801 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
    10861 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
    10921 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
    10981 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
    11041 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
    11101 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
    11161 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
    11221 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
    11281 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
    11341 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    11401 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    11461 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    11521 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gtcgacctcg agggggggcc
    11581 cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
    11641 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    11701 ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    11761 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    11821 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    11881 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    11941 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    12001 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    12061 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12121 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12181 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12241 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12301 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12361 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12421 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12481 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    12721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    12781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    12841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    12901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    12961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    13021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    13081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    13141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    13201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    13261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    13321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    13381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    13441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    13501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    13561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    13621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    13681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    13741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
    13801 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
    13861 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
    13921 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
    13981 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
    14041 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
    14101 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
    14161 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
    14221 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
    14281 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    14341 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    14401 cagtgagcgc gcgtaatacg actcactata gggcgaattg gagctccacc gcggtggcgg
    14461 ccgctctagc caaccttgag tcccttagca tctatagtgt ctctttctgc ttctttagca
    14521 ttccctatag tcatgtactg gaaccctact agaaccaggg ttggctgcca gtctagtccc
    14581 ttctgcctag agagagcagc gagggacagt ggggtgccag acatcccact ccagggtggg
    14641 aacggggcct ggattttgtt tttttttttg gntttttttt tttttttttt ttgagacaga
    14701 gtgtcctg
//
