LOCUS       sequence-204982-        2371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pPGK1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK011
FEATURES             Location/Qualifiers
     source          1..2371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(214..802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(294..313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             984..1643
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1050..1069)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1714..1748
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gataatagta tgagggtgaa gccaaaataa aggattcgcg cccaaatcgg catctttaaa
       61 tgcaggtatg cgatagttcc tcactctttc cttactcacc gtttgagacc gactacggtt
      121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      841 gtggaacgaa aactcacgtt aagggatttt ggtcatgatt gatcgggcac gtaagaggtt
      901 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      961 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1021 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1081 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1141 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1201 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1261 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1321 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1381 atttccctaa agggtttatt gagaatatgt ttttcgtttc agccaatccc tgggtgagtt
     1441 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1501 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1561 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1621 atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat
     1681 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     1741 ttttttattg gtctggtctc acatagatct tgttttatat ttgttgtaaa aagtagataa
     1801 ttacttcctt gatgatctgt aaaaaagaga aaaagaaagc atctaagaac ttgaaaaact
     1861 acgaattaga aaagaccaaa tatgtatttc ttgcattgac caatttatgc aagtttatat
     1921 atatgtaaat gtaagtttca cgaggttcta ctaaactaaa ccaccccctt ggttagaaga
     1981 aaagagtgtg tgagaacagg ctgttgttgt cacacgattc ggacaattct gtttgaaaga
     2041 gagagagtaa cagtacgatc gaacgaactt tgctctggag atcacagtgg gcatcatagc
     2101 atgtggtact aaaccctttc ccgccattcc agaaccttcg attgcttgtt acaaaacctg
     2161 tgagccgtcg ctaggacctt gttgtgtgac gaaattggaa gctgcaatca ataggatgac
     2221 aggaagtcga gcgtgtctgg gttttttcag ttttgttctt tttgcaaaca aatcacgagc
     2281 gacggtaatt tctttctcga taagaggcca cgtgctttat gagggtaaca tcaattcaag
     2341 aaggagggaa acacttcctt tttctggccc t
//
