LOCUS       sequence-204994-       12921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway vector for generating cyan fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 12921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Ch
FEATURES             Location/Qualifiers
     source          1..12921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    383..404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(419..424,425..442,443..449)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    457..473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     462..484
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     481..497
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     481..497
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    594..618
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(742..747,748..765,766..772)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             826..1485
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(892..911)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1827..2132
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2011..2030
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2173..2297)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2317..2319,2320..2322,2323..3036)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2362..2383)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2623..2642)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2970..2991
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      3190..3761
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     complement(3794..3811)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3794..3810)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3803..3825)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4013..4037
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5338..5967
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6396..7469
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7535..7729
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7965..7987)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8073..8213
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8128..8147
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8228..8245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8399..8987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8479..8498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9074..9868)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11492..11516
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11594..11768
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11799..12824)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 gcttgacaaa cgcaccaagt tatcgtgcac caagcagcag atgataataa tgtcctcgtt
       61 cctgtctgct aataagagtc acacttcgag cgccgccgct actgctacaa gtggggctga
      121 tctgaccagt tgcctaaatg aaccatcttg tcaaacgaca caaattttgt gctcaccgcc
      181 tggacgacta aaccaaaata ggcattcatt gttgacctcc actagctcca gccaagccca
      241 aaaaatgctc cttcaatatc agttttggca agctgctcta gccaatacgc aaaccgcctc
      301 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      361 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      421 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      481 caggaaacag ctatgacatg attacgaatt cgagctctcc catatggtcg acctgcaggc
      541 ggccgcgaat tcactagtga tttataagct tacgaacgat agacatctgg atcacaagtt
      601 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
      661 ttttgcataa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
      721 gccgcattag gcaccccagg ttttacactt tatgcttccg gctcgtataa tgtgtggatt
      781 ttgagttagg atccgtcgag attttcagga gctaaggaag ttaaaatgga gaaaaaaatc
      841 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      901 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      961 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     1021 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     1081 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     1141 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     1201 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     1261 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     1321 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     1381 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     1441 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc
     1501 ttactaaaag tcagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     1561 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     1621 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     1681 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     1741 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     1801 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     1861 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     1921 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     1981 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     2041 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     2101 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     2161 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     2221 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     2281 ttcttgtaca aagtggtgat agggcccggg atcctgatgg tgagcaaggg cgaggagctg
     2341 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     2401 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     2461 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
     2521 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     2581 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2641 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2701 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc
     2761 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     2821 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2881 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     2941 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3001 gggatcactc tcggcatgga cgagctgtac aagtgagatc cacttaacgt tactgaaatc
     3061 atcaaacagc ttgacgaatc tggatataag atcgttggtg tcgatgtcag ctccggagtt
     3121 gagacaaatg gtgttcagga tctcgataag atacgttcat ttgtccaagc agcaaagagt
     3181 gccttctagt gatttaatag ctccatgtca acaagaataa aacgcgtttc gggtttacct
     3241 cttccagata cagctcatct gcaatgcatt aatgcattgg acctcgcaac cctagtacgc
     3301 ccttcaggct ccggcgaagc agaagaatag cttagcagag tctattttca ttttcgggag
     3361 acgagatcaa gcagatcaac ggtcgtcaag agacctacga gactgaggaa tccgctcttg
     3421 gctccacgcg actatatatt tgtctctaat tgtactttga catgctcctc ttctttactc
     3481 tgatagcttg actatgaaaa ttccgtcacc agcccctggg ttcgcaaaga taattgcact
     3541 gtttcttcct tgaactctca agcctacagg acacacattc atcgtaggta taaacctcga
     3601 aaatcattcc tactaagatg ggtatacaat agtaaccatg catggttgcc tagtgaatgc
     3661 tccgtaacac ccaatacgcc ggccgaaact tttttacaac tctcctatga gtcgtttacc
     3721 cagaatgcac aggtacactt gtttagaggt aatccttctt tctagagtcg acctgcaggc
     3781 atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3841 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3901 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct
     3961 tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga
     4021 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
     4081 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     4141 ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
     4201 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
     4261 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
     4321 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
     4381 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
     4441 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
     4501 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
     4561 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
     4621 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
     4681 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
     4741 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
     4801 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
     4861 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
     4921 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
     4981 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
     5041 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
     5101 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga
     5161 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
     5221 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
     5281 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
     5341 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
     5401 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
     5461 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
     5521 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
     5581 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
     5641 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
     5701 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
     5761 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
     5821 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
     5881 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
     5941 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
     6001 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
     6061 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
     6121 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
     6181 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
     6241 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
     6301 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc
     6361 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc
     6421 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
     6481 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
     6541 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
     6601 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
     6661 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
     6721 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
     6781 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
     6841 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
     6901 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
     6961 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
     7021 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
     7081 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
     7141 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
     7201 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
     7261 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
     7321 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
     7381 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
     7441 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
     7501 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
     7561 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
     7621 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
     7681 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
     7741 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
     7801 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
     7861 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
     7921 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     7981 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8041 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     8101 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8161 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     8221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8821 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9061 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
     9121 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
     9181 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
     9241 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
     9301 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
     9361 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
     9421 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
     9481 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
     9541 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
     9601 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
     9661 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
     9721 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
     9781 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
     9841 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
     9901 aaagataccc caagaagcta aggaaggtgc gaacaagtcc ctgatatgag atcatgtttg
     9961 tcatctggag ccatagaaca gggttcatca tgagtcatca acttaccttc gccgacagtg
    10021 aattcagcag taagcgccgt cagaccagaa aagagatttt cttgtcccgc atggagcaga
    10081 ttctgccatg gcaaaacatg gtggaagtca tcgagccgtt ttaccccaag gctggtaatg
    10141 gccggcgacc ttatccgctg gaaaccatgc tacgcattca ctgcatgcag cattggtaca
    10201 acctgagcga tggcgcgatg gaagatgctc tgtacgaaat cgcctccatg cgtctgtttg
    10261 cccggttatc cctggatagc gccttgccgg accgcaccac catcatgaat ttccgccacc
    10321 tgctggagca gcatcaactg gcccgccaat tgttcaagac catcaatcgc tggctggccg
    10381 aagcaggcgt catgatgact caaggcacct tggtcgatgc caccatcatt gaggcaccca
    10441 gctcgaccaa gaacaaagag cagcaacgcg atccggagat gcatcagacc aagaaaggca
    10501 atcagtggca ctttggcatg aaggcccaca ttggtgtcga tgccaagagt ggcctgaccc
    10561 acagcctggt caccaccgcg gccaacgagc atgacctcaa tcagctgggt aatctgctgc
    10621 atggagagga gcaatttgtc tcagccgatg ccggctacca aggggcgcca cagcgcgagg
    10681 agctggccga ggtggatgtg gactggctga tcgccgagcg ccccggcaag gtaagaacct
    10741 tgaaacagca tccacgcaag aacaaaacgg ccatcaacat cgaatacatg aaagccagca
    10801 tccgggccag ggtggagcac ccatttcgca tcatcaagcg acagttcggc ttcgtgaaag
    10861 ccagatacaa ggggttgctg aaaaacgata accaactggc gatgttattc acgctggcca
    10921 acctgtttcg ggcggaccaa atgatacgtc agtgggagag atctcactaa aaactgggga
    10981 taacgcctta aatggcgaag aaacggtcta aataggctga ttcaaggcat ttacgggaga
    11041 aaaaatcggc tcaaacatga agaaatgaaa tgactgagtc agccgagaag aatttccccg
    11101 cttattcgca ccttccctaa ttataacaag acgaactcca attcactgtt ccttgcattc
    11161 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
    11221 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
    11281 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
    11341 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
    11401 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
    11461 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
    11521 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg
    11581 aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat tagaaatttt
    11641 attgatagaa gtattttaca aatacaaata catactaagg gtttcttata tgctcaacac
    11701 atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca cacattatta
    11761 tggagaaact cgaaactggt tcccggtcgg catctactct attcctttgc cctcggacga
    11821 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
    11881 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
    11941 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
    12001 tagagttggt caagaccaat gcggagcata tacgcccgga ggcgcggcga tcctgcaagc
    12061 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
    12121 cagaagagga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
    12181 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcatgc
    12241 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
    12301 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
    12361 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
    12421 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg
    12481 accggctgga gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
    12541 gcacggcggg agatgcaata ggtcaggctc tcgctgaact ccccaatgtc aagcacttcc
    12601 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
    12661 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
    12721 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
    12781 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatttggat gcttgggtag
    12841 aataggtaag tcagattgaa tctgaaataa agggaggaag ggcgaactta agaaggtatg
    12901 accgggtcgt ccacttacct t
//
