LOCUS       sequence-204995-        5491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV packaging vector to express mNeonGreen in astrocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5491)
  TITLE     AAV-GFAP-mNeonGreen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV-GFAP-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..5491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      153..741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     642..661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   803..932
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1661..1670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1922..1931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2420..3008
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2473..2493)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2891..2902)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3040..3516
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3239..3258)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3556..3696
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3556..3685
                     /label=AAV2 ITR
     rep_origin      3771..4226
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3858..3877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4068..4089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4243..4262)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4362..4384
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4422..4440)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4508..4612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4613..4681,4682..5473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
       61 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      481 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      541 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      601 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      661 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      721 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      781 tgctggcctt ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc
      841 gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga
      901 gggagtggcc aactccatca ctaggggttc ctgcggccgc acgcgtgatc taacatatcc
      961 tggtgtggag taggggacgc tgctctgaca gaggctcggg ggcctgagct ggctctgtga
     1021 gctggggagg aggcagacag ccaggccttg tctgcaagca gacctggcag cattgggctg
     1081 gccgcccccc agggcctcct cttcatgccc agtgaatgac tcaccttggc acagacacaa
     1141 tgttcggggt gggcacagtg cctgcttccc gccgcacccc agcccccctc aaatgccttc
     1201 cgagaagccc attgagcagg gggcttgcat tgcaccccag cctgacagcc tggcatcttg
     1261 ggataaaagc agcacagccc cctaggggct gcccttgctg tgtggcgcca ccggcggtgg
     1321 agaacaaggc tctattcagc ctgtgcccag gaaaggggat caggggatgc ccaggcatgg
     1381 acagtgggtg gcaggggggg agaggagggc tgtctgcttc ccagaagtcc aaggacacaa
     1441 atgggtgagg ggagagctct ccccatagct gggctgcggc ccaaccccac cccctcaggc
     1501 tatgccaggg ggtgttgcca ggggcacccg ggcatcgcca gtctagccca ctccttcata
     1561 aagccctcgc atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc
     1621 tccgctgctc gcggggtcta gagtcgacaa gcttggatcc gccaccatgg tttccaaggg
     1681 cgaagaggac aatatggcca gcctgcctgc cacacacgag ctgcacatct ttggcagcat
     1741 caacggcgtg gacttcgaca tggttggaca aggcaccggc aatcccaacg acggctacga
     1801 ggaactgaac ctgaagtcca caaagggcga cctgcagttc agcccttgga ttctggtgcc
     1861 tcacatcggc tacggcttcc accagtacct gccttatcct gacggcatga gcccttttca
     1921 ggccgccatg gtggatggct ctggctacca ggttcaccgg accatgcagt ttgaagatgg
     1981 cgccagcctg accgtgaact acagatacac atacgagggc agccacatca aaggcgaggc
     2041 ccaagtgaaa ggcacaggct ttccagccga tggacccgtg atgaccaata gcctgacagc
     2101 cgccgactgg tgcagaagca agaaaacata ccccaacgac aagaccatca tcagcacctt
     2161 caagtggtcc tacaccaccg gcaacggcaa gagatacaga agcaccgcca gaaccaccta
     2221 caccttcgcc aaacctatgg ccgccaacta cctgaagaac cagcctatgt acgtgttccg
     2281 caagaccgag ctgaagcaca gcaagacaga actgaacttc aaagagtggc agaaagcctt
     2341 caccgacgtg atgggcatgg acgagctgta taagtaggct agcctcgagg atatcgaatt
     2401 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2461 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2521 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2581 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2641 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2701 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2761 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2821 gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg
     2881 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2941 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3001 ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc
     3061 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     3121 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     3181 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     3241 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     3301 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     3361 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     3421 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     3481 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc
     3541 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     3601 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     3661 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     3721 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3781 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3841 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3901 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3961 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     4021 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4081 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     4141 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4201 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4261 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4321 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4381 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4441 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4501 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4561 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4621 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4681 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4741 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4801 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4861 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4921 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4981 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5041 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5101 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5161 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5221 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5281 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5341 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5401 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5461 taagcattgg taactgtcag accaagttta c
//
