LOCUS       sequence-204999-       13085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter for BmalI promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Brown SA, Fleury-Olela F, Nagoshi E, Hauser C, Juge C, Meier CA, 
            Chicheportiche R, Dayer JM, Albrecht U, Schibler U
  TITLE     The period length of fibroblast circadian gene expression varies 
            widely among human individuals.
  JOURNAL   PLoS Biol. 2005 Oct;3(10):e338. Epub 2005 Sep 27.
  PUBMED    16167846
REFERENCE   2  (bases 1 to 13085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2005-10"; volume: "3(10)"; pages: "e338. Epub 2005 
            Sep 27"
COMMENT     Sequence Label: pABpuro-BluF
FEATURES             Location/Qualifiers
     source          1..13085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    158..292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(195..214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     249..268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             460..1093
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1140..1265
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1758..1991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2176..2220
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     regulatory      3632..3641
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3638..5290
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3704..3722)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        6416..6745
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6596..6731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6755..7354
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6755..6774)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7251..7271
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7425..8013
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7478..7498)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7896..7907)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8106..8339
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8364..8382)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8365..8382)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8672..8691)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8905..8924)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9084..9102)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9170..9274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9275..9343,9344..10135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9493..9512)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10306..10894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10795..10814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11048..11065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        11140..11469
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11320..11455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12603..12668
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12798..12818
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
       61 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      121 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      181 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      241 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
      301 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
      361 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
      421 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
      481 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
      541 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
      601 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
      661 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
      721 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
      781 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
      841 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
      901 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      961 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1021 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1081 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1141 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1201 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1261 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1321 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1381 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1441 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1501 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1561 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1621 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1681 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1741 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1801 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1861 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1921 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1981 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     2041 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     2101 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     2161 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2221 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2281 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2341 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2401 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     2461 aacggatctc gacggtatcg gatctcgagc tcaagcttcg aattcgccct tgggctacaa
     2521 cagaacaact aaccacccaa acaaacaact ttgcaacttt tctttctttc ccgctccttt
     2581 gatacttttg caaagtacat ttgctagggc catgtccata acatgtaata gaatcttgct
     2641 caggtctcac cctgtggccc cggctagctc agtactcgcg attatgcccc tgcctcagct
     2701 tcttgagggt tggaattaca gactacgcca ccacccccgg gagacttctc ttccgatcgg
     2761 gaatgacagc tccactggac gcttggaggt caagagaaaa aaaaaaaaaa aaaaaaaaaa
     2821 ngaacgcgcg atccggttag tcgtcggaag gaaaagtctt ctaaatgcgc tggctattag
     2881 cgctgtggtt ccgactagga caagccaggg gtttcaggaa ggttctgtca ctctgtgttc
     2941 ctaatatgtg gtttcctaga tggagctgga gagggatggg cgaagagatg cggcgttttc
     3001 tcagtgttct gcgagccagg aaggggacgg aggaggaggg agagctgaga ggcagtcact
     3061 agccagggac gcggctgagg agcaggggac aacggcgagc tcgcagagtc cgcaacgcag
     3121 tggcctcagc gagctttaga cctgaggggg aaaaccgagg gctggggcaa gaaatccaca
     3181 gagcgtgcca attggtccac tcctcggggc gtgtgcttct gtgcgccaaa tgattggtga
     3241 agggaaagta gcaggtaaac cagccctgcc gtcttgccat tggtcagagg ctttcctatc
     3301 ggtcactcgg ttggctagcc taacgcagag cagaacgcga attggtttgg gttgtccgcc
     3361 aagacaactc cgttcgctct ctctgattgg ctaacgggaa gaggcaggta tccgggctgc
     3421 gcggctcctc cattggtggg cggggaaggg gggttgggca cagcgattgg tgggcggggg
     3481 gccgggcctg ggccggcggg gagcggattg gtcggaaagt aggttagtgg tgcgacattt
     3541 agggaaggca gaaagtaggt cagggacgga ggtgcctgtt tacccgcgcc ggacttgccg
     3601 ccgccgccgc cgcgggatcg atccaccggt cgccaccatg gaagacgcca aaaacataaa
     3661 gaaaggcccg gcgccattct atcctctaga ggatggaacc gctggagagc aactgcataa
     3721 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
     3781 ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa
     3841 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     3901 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     3961 ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt
     4021 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa
     4081 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     4141 cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg
     4201 tgacaaaaca attgcactga taatgaattc ctctggatct actgggttac ctaagggtgt
     4261 ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg
     4321 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     4381 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     4441 tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt
     4501 accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     4561 tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc
     4621 aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc
     4681 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     4741 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg
     4801 cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac
     4861 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     4921 agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatatca
     4981 ggtggccccc gctgaattgg aatcgatatt gttacaacac cccaacatct tcgacgcggg
     5041 cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     5101 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     5161 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     5221 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc
     5281 caaattgtaa gcggccgcca gtgtgatgga tatctgcaga attcgccctt agacactaca
     5341 tttgctttgg caacagctgc agtatcaaag tgcattaatg gtgaagtttt acagtctgtg
     5401 aagcttactg gatagagaga gaacagcttt tatgtactga ctccataaaa gccacctcag
     5461 agccattgat acaagtcaat ctaccatgtg taacttcaga caaagtggaa ctaaacctgc
     5521 tccagtgttt cctcatcatt gagtttgggc tagctgtgga tagcttgcat taattgtata
     5581 ttttggattt tgtttgtgtt gaatttttta atcattgtgc acagaatcat catcggtagc
     5641 ttttatatgc aaaatggcca tttcagatgt atggtgtttt tacactacaa agaagtcccc
     5701 catgtggata tttcttatac taattgtatc ataaagctgt ttattcttcc ttgtaagaat
     5761 cctttactat aaatatgggt taaagtataa tgtattagac agttaaatat ttttaataaa
     5821 tgtttccctt gttctataag tactgttccc aattgataat taggaagaaa atcttcatat
     5881 gctacagggc tggttcatcc acttcaccag cggtctaaac acattcctgc tgttatgttt
     5941 acaatgtcct tctgctctta taatgacttc ttccatgcta ccagcttaga catattataa
     6001 aaatagctca cttccaaact gtagccacct caacttatat gaaaacaaag atatcaactc
     6061 caatttccag tgcttagatt tcaagattac aagctggctt ctctagacct gcagttacat
     6121 ggtgtgctca agtctaccag gggtagatgt aagcgacggc atctgctctc tctttctatg
     6181 gtcccatcga caaactgaga tgtgatccca ccaaactaaa gttcactctg gaaaccaatg
     6241 ggttggttta ctgggcttcc atacagacac aggcgagagg ttccctacag gtatgctcct
     6301 actcaaaggc cacactagaa agcctttact ggggctggaa gggcgaattc cagcacactg
     6361 gcggccgtta ctagtggatc cgaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg
     6421 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6481 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6541 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6601 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6661 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6721 ttttggaggc ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg
     6781 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6841 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6901 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6961 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7021 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7081 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7141 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7201 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7261 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7321 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     7381 gaccgaaagg agcgcacgac cccatgcatc gaattaattc cgataatcaa cctctggatt
     7441 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7501 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7561 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7621 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7681 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7741 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7801 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     7861 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7921 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7981 cgagtcggat ctccctttgg gccgcctccc cgcatcggga attaattcga gctcggtacc
     8041 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8101 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8161 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8221 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8281 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8341 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     8401 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     8461 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     8521 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     8581 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     8641 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     8701 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     8761 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     8821 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     8881 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     8941 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     9001 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     9061 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     9121 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9181 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9241 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     9301 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     9361 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     9421 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     9481 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     9541 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     9601 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     9661 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     9721 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     9781 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     9841 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     9901 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9961 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10021 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10081 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10141 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10201 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    10261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    10321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    10381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    10441 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    10501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    10561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    10621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    10681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    10741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    10801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    10861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    10921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    10981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    11041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    11101 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
    11161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    11221 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    11281 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    11341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    11401 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    11461 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
    11521 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
    11581 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
    11641 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
    11701 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
    11761 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
    11821 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
    11881 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
    11941 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
    12001 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
    12061 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
    12121 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
    12181 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
    12241 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
    12301 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
    12361 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
    12421 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
    12481 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
    12541 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
    12601 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
    12661 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
    12721 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
    12781 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
    12841 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
    12901 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
    12961 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
    13021 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
    13081 tttta
//
