LOCUS       sequence-205-exp        4171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3788
FEATURES             Location/Qualifiers
     source          1..4171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(542..570)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1318..1338
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        1916..2020
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2021..2089,2090..2881)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2239..2258)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3052..3640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3541..3560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3794..3811
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3928..3949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3964..3969,3970..3987,3988..3994)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4002..4018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4007..4029
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4026..4042
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4026..4042
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcaccaa aaacggaacg ttgagctgga
       61 cggaaatagt ggtaaagtga catgattata gtttgaagat ttctaatttc acaattagag
      121 caaatgttgt tcggtattta ttttcaacgg tatttatact attttccacc tttttctaga
      181 acattcgagc tgcttgttgc aaaaggaggg cgactcacat tcggtacatg gaaaagtagt
      241 gtacacaata aagagaccca gatacatttt ccgtctgcgt ctctttgcac ccaccgggag
      301 tattttcaaa cgaatgcatc taggaccttc tagaacattc tgtaaggctg cagaatgcgg
      361 gtatataagg aaagcgggct cagaggaagc caacacgctt tgttctagtg catctaaaaa
      421 acttcgaaaa tcctcatcgg gatcctatcg attcgcggcc gctgtacacc cgggtgccct
      481 atagtgagtc gtattggtac cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg
      541 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      601 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      661 tggaaaacta cctgttccat gggtaagttt aaacatatat atactaacta accctgatta
      721 tttaaatttt cagccaacac ttgtcactac tttctgttat ggtgttcaat gcttctcgag
      781 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      841 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca
      901 gttcggtact aactaaccat acatatttaa attttcaggt gctgaagtca agtttgaagg
      961 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1021 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1081 acaaaagaat ggaatcaaag ttgtaagttt aaacatgatt ttactaacta actaatctga
     1141 tttaaatttt cagaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     1201 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     1261 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     1321 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggacgaac tatacaaaag
     1381 cccacaagct ttggaattcg ctagccggcc atacaagtaa tccggatgat cgacgccaac
     1441 gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat tatttattta
     1501 ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa tctaaacatt
     1561 tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc
     1621 aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg
     1681 gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa
     1741 ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa
     1801 actagtaaaa aataagcaaa gaaattaggc gaacgatgag aattgtcctc gcttgggccc
     1861 aaaaggcctt ttttttttgg cgccgacgtc aggtggcact tttcggggaa atgtgcgcgg
     1921 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1981 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2041 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2101 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2161 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2221 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2281 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2341 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2401 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2461 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2521 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2581 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2641 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2701 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2761 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2821 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2881 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2941 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3001 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3181 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3421 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     3481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3601 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3661 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3721 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3781 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     3841 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     3901 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     3961 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     4021 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
     4081 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
     4141 gatggatacg ctaacaactt ggaaatgaaa t
//
