LOCUS       sequence-20501-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(32..156)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(164..180)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(164..180)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(186..449)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(269..292)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 tgtaaagcct gggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca
       61 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
      121 ttacgtttct cgttcagctt ttttgtacaa acttgtgatg ggggatccac tagttctaga
      181 atccgtcgaa actaagttct ggtgttttaa aactaaaaaa aagactaact ataaaagtag
      241 aatttaagaa gtttaagaaa tagatttaca gaattacaat caatacctac cgtctttata
      301 tacttattag tcaagtaggg gaataatttc agggaactgg tttcaacctt ttttttcagc
      361 tttttccaaa tcagagagag cagaaggtaa tagaaggtgt aagaaaatga gatagataca
      421 tgcgtgggtc aattgccttg tgtcatcatt tactccaggc aggttgcatc actccattga
      481 ggttgtgccc gttttttgcc tgtttgtgcc cctgttctct gtagttgcgc taagagaatg
      541 gacctatgaa ctgatggttg gtgaagaaaa caatattttg gtgctgggat tctttttttt
      601 tctggatgcc agcttaaaaa gcgggctcca ttatatttag tggatgccag gaataaactg
      661 ttcacccaga cacctacgat gttatatatt ct
//
