LOCUS       sequence-20504-e         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG426GAL-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(128..252)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(329..350)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(590..609)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctcgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
       61 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      121 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
      181 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
      241 gtacaaagtg gtgatgggct gcaggaattc gatatcaagc ttaatggtga gcaagggcga
      301 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      361 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      421 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      481 ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      541 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      601 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      661 ga
//
