LOCUS       sequence-205094-        6559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminus to aa326 of TP901 integrase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pSD35_pCAG-PV1-TP901-N326-L1-FRB-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..38)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(22..38)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(35..57)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    46..62
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(70..76,77..94,95..100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      221..355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    375..509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(412..431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     466..485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(577..594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(748..1336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(828..847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1507..2298,2299..2367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2130..2149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2368..2472)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2503..2882
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2884..3159
                     /label=chicken beta-actin promoter
     intron          3160..4175
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4099..4121
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4183..4202
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5330..5605
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRIS"
     CDS             5606..5626
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5636..5860
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6100..6119)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6165..6220
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6219..6238)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
       61 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      181 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      301 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
      361 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      421 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      481 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
      541 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      601 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      661 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      721 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      781 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      841 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      901 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      961 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1021 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1081 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1141 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1201 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1261 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1321 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1381 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1441 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1501 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1561 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1621 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1681 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1741 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1801 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1861 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1921 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1981 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2041 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2101 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2161 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2221 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2281 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2341 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2401 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2461 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattgacta
     2521 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2581 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2641 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2701 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2761 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2821 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2881 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
     2941 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     3001 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt
     3061 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     3121 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gttgccttcg
     3181 ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     3241 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
     3301 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
     3361 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     3421 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     3481 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     3541 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     3601 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
     3661 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     3721 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     3781 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     3841 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     3901 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     3961 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     4021 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     4081 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     4141 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     4201 tgctgtctca tcattttggc aaagaattga ttaattcgag cgaaggcgcg cccattactc
     4261 gcatccattc tcaggctgtc tcgtctcgtc tcgcacgcgt gccgccacca tgaccaagaa
     4321 ggtggccatc tacaccagag tgtccaccac caaccaggcc gaggaaggct tcagcatcga
     4381 cgagcagatc gaccggctga ccaaatacgc cgaggccatg ggatggcagg tgtccgatac
     4441 ctacaccgac gccggcttta gcggcgccaa gctggaaaga cccgccatgc agcggctgat
     4501 caacgacatc gagaacaagg ccttcgacac cgtgctggtg tacaagctgg acaggctgag
     4561 cagaagcgtg cgggacaccc tgtacctcgt gaaggacgtg ttcaccaaga acaagatcga
     4621 cttcatcagc ctgaacgaga gcatcgacac cagcagcgct atgggcagcc tgttcctgac
     4681 catcctgagc gccatcaacg agttcgagcg cgagaacatc aaagaacgga tgaccatggg
     4741 caagctgggc agagccaaga gcggcaagag catgatgtgg accaagaccg ccttcggcta
     4801 ctaccacaac agaaagaccg gcatcctgga aatagtgcca ctgcaggcca ccatcgtgga
     4861 acagatcttc accgactacc tgagcggcat ctccctgacc aagctgagag acaagctgaa
     4921 cgagtccggc cacatcggca aggacatccc ttggagctac cggaccctgc ggcagaccct
     4981 ggacaaccct gtgtactgcg gctacatcaa gttcaaggac tccctgttcg agggcatgca
     5041 caagcccatc atcccttacg agacatacct gaaggtgcag aaagagctgg aagagagaca
     5101 gcagcagacc tacgagcgga acaacaaccc cagacccttc caggccaagt acatgctgtc
     5161 cggcatggcc agatgcggct actgtggcgc ccctctgaag atcgtgctgg gccacaagag
     5221 aaaggacggc agccggacca tgaagtacca ctgcgccaac cggttcccta gaaagaccaa
     5281 gggcatctcc ggagggtctg gctccggatc aagtggtggc agcggtacca tcctctggca
     5341 tgagatgtgg catgaaggcc tggaagaggc atctcgtttg tactttgggg aaaggaacgt
     5401 gaaaggcatg tttgaggtgc tggagccctt gcatgctatg atggaacggg gcccccagac
     5461 tctgaaggaa acatccttta atcaggccta tggtcgagat ttaatggagg cccaagagtg
     5521 gtgcaggaag tacatgaaat cagggaatgt caaggacctc ctccaagcct gggacctcta
     5581 ttatcatgtg ttccgacgaa tctcacccaa gaaaaagcgg aaggtgtgag aattcctgtg
     5641 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5701 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5761 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5821 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5881 agctggggct ctagggggta tccccacgcg ccctgtagcg tgtaccgctg ggagttcgta
     5941 gacggaaaca aacgcagaat ccaagcgcgg ccgcccagga tacatagatt accacaactc
     6001 cgagcccttc caccgctagc gcactcctca ggtgcaggct gcctatcaga aggtggtggc
     6061 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     6121 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     6181 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     6241 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     6301 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     6361 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     6421 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     6481 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     6541 gagatccctc gacctgcag
//
