LOCUS       sequence-205173-        4160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid can be used for Drosophila S2 driven secretion of 
            extracellular part of human interferon-_ receptor 2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Mikulecky P, Zahradnik J, Kolenko P, Cerny J, Charnavets T, Kolarova
            L, Necasova I, Pham PN, Schneider B
  TITLE     Crystal structure of human interferon-gamma receptor 2 reveals the 
            structural basis for receptor specificity.
  JOURNAL   Acta Crystallogr D Struct Biol. 2016 Sep;72(Pt 9):1017-25. doi: 
            10.1107/S2059798316012237. Epub 2016 Aug 18.
  PUBMED    27599734
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr D Struct Biol."; date: "2016-09"; volume: "72(Pt 
            9):1017-25. doi"; pages: " 10.1107/S2059798316012237. Epub 2016 Aug 
            18"
COMMENT     Sequence Label: pMTgR2
FEATURES             Location/Qualifiers
     source          1..4160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..67
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     60..76
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        82..508
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     526..579
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             1252..1269
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1292..1309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1342..1476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1366..1385)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1420..1439
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1620..1636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1620..1636)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1633..1655)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1644..1660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1668..1674,1675..1692,1693..1698))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1713..1734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1851..1868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2022..2610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2102..2121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2781..3572,3573..3641))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3404..3423
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3642..3746)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3814..3832
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3870..3892)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3992..4011
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
       61 taaaacgacg gccagtgaat taattcgttg caggacagga tgtggtgccc gatgtgacta
      121 gctctttgct gcaggccgtc ctatcctctg gttccgataa gagacccaga actccggccc
      181 cccaccgccc accgccaccc ccatacatat gtggtacgca agtaagagtg cctgcgcatg
      241 ccccatgtgc cccaccaaga gctttgcatc ccatacaagt ccccaaagtg gagaaccgaa
      301 ccaattcttc gcgggcagaa caaaagcttc tgcacacgtc tccactcgaa tttggagccg
      361 gccggcgtgt gcaaaagagg tgaatcgaac gaaagacccg tgtgtaaagc cgcgtttcca
      421 aaatgtataa aaccgagagc atctggccaa tgtgcatcag ttgtggtcag cagcaaaatc
      481 aagtgaatca tctcagtgca actaaagggg ggatccgatc tcaatatgaa gttatgcata
      541 ttactggccg tcgtggcctt tgttggcctc tcgctcggga gatcttccca gctgcccgct
      601 cctcagcacc cgaagattcg cctgtacaac gcagagcagg tcctgagttg ggagccagtg
      661 gccctgagca atagcacgag gcctgttgtc taccaagtgc agtttaaata caccgacagt
      721 aaatggttca cggccgacat catgtccata ggggtgaatt gtacacagat cacagcaaca
      781 gagtgtgact tcactgccgc cagtccctca gcaggcttcc caatggattt caatgtcact
      841 ctacgccttc gagctgagct gggagcactc cattctgcct gggtgacaat gccttggttt
      901 caacactatc ggaatgtgac tgtcgggcct ccagaaaaca ttgaggtgac cccaggagaa
      961 ggctccctca tcatcaggtt ctcctctccc tttgacatcg ctgatacctc cacggccttt
     1021 ttttgttatt atgtccatta ctgggaaaaa ggaggaatcc aacaggtcaa aggccctttc
     1081 agaagcaact ccatttcatt ggataactta aaaccctcca gagtgtactg tttacaagtc
     1141 caggcacaac tgctttggaa caaaagtaac atctttagag tcgggcattt aagcaacata
     1201 tcttgctacg aaacaatggc agatgcctcc actgagcttc agcaaaccgg tcatcatcac
     1261 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa ggcctgagct
     1321 cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga gtttggacaa
     1381 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     1441 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     1501 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     1561 tgtggtatgg ctgattatga tcagtcgacc tgcaggcatg caagcttggc gtaatcatgg
     1621 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1681 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1741 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1801 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1861 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1921 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1981 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2041 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2101 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2161 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2221 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2281 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2341 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2401 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2461 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2521 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2581 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2641 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2701 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2761 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2821 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2881 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2941 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3001 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3061 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3121 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3181 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3241 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3301 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3361 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3421 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3481 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3541 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3601 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3661 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3721 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     3781 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     3841 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     3901 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     3961 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     4021 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     4081 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     4141 ttacgccagc tggcgaaagg
//
