LOCUS       sequence-205205-       10159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HACK Donor plasmids for converting GAL4 lines to QF2 lines using 
            HACK method. Updated version of Addgene#80275 for easier cloning of 
            inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 10159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pHACK-GAL4>QF2(newHA1)
FEATURES             Location/Qualifiers
     source          1..10159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    41..74
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    88..121
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    290..323
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             1532..1585
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(2918..2951)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3015..3136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3026..3045)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3080..3099
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3256..3966)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3803..3821
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(4144..4177)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5212..5556
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    5566..5599
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        5615..6411
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        6431..6506
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6507..6927
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        6948..7023
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(7456..7489)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7959..7976)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7959..7975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8074..8091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8245..8833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8325..8344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8955..9746,9747..9815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9578..9597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9816..9907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9949..9968
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9974..9993
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gggctaatta tggggtgtcg cccttattcg actctatagt gaagttccta ttctctagaa
       61 agtataggaa cttctgaagt ggggcgggtg ccagggcgtg cccttgggct ccccgggcgc
      121 gtacaagccg aaggcgcgca tgatgaaata acataaggtg gtcccgtcga aagccgaagc
      181 ttaccgaagt atacacttaa attcagtgca cgtttgcttg ttgagaggaa aggttgtgtg
      241 cggacgaatt tttttttgaa aacattaacc cttacgtaac gcgagatctg aagttcctat
      301 tctctagaaa gtataggaac ttctagggat aacagggtaa tagatctgga tcctaatgat
      361 gacatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc
      421 caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct
      481 gactagggca catctgacag aagtggaatc aaggctagaa agactggaac agctatttct
      541 actgattttt cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat
      601 aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga
      661 tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc
      721 gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgattga
      781 ctcggcagct catcatgata actccacaat tccgttggat tttatgccca gggatgctct
      841 tcatggattt gattggtctg aagaggatga catgtcggat ggcttgccct tcctgaaaac
      901 ggaccccaac aataatgggt tctttggcga cggttctctc ttatgtattc ttcgatctat
      961 tggctttaaa ccggaaaatt acacgaactc taacgttaac aggctcccga ccatgattac
     1021 ggatagatac acgttggctt ctagatccac aacatcccgt ttacttcaaa gttatctcaa
     1081 taattttcac ccctactgcc ctatcgtgca ctcaccgacg ctaatgatgt tgtataataa
     1141 ccagattgaa atcgcgtcga aggatcaatg gcaaatcctt tttaactgca tattagccat
     1201 tggagcctgg tgtatagagg gggaatctac tgatatagat gttttttact atcaaaatgc
     1261 taaatctcat ttgacgagca aggtcttcga gtcaggttcc ataattttgg tgacagccct
     1321 acatcttctg tcgcgatata cacagtggag gcagaaaaca aatactagct ataattttca
     1381 cagcttttcc ataagaatgg ccatatcatt gggcttgaat agggacctcc cctcgtcctt
     1441 cagtgatagc agcattctgg aacaaagacg ccgaatttgg tggtctgtct actcttggga
     1501 gatccaattg tccctgcttg gatcctcgcg tgagggccgc ggcagcctgc tgacctgcgg
     1561 cgatgtggag gagaaccccg ggccccctag gatgccaccc aagcgcaaaa cgcttaacgc
     1621 tgcggctgag gctaacgctc atgccgacgg acacgccgac ggaaacgccg acggacacgt
     1681 ggccaatacg gccgcgtcct cgaataatgc gaggttcgct gatctcacta acatcgatac
     1741 tccgggtctg ggacccacaa ctacgaccct gctcgtggaa ccagcacgct caaagcgtca
     1801 acgagtgtcc cgcgcatgcg accagtgccg tgcagcccga gagaaatgcg acggaataca
     1861 gcctgcgtgt ttcccgtgcg tttcccaggg aaggtcctgc acttatcagg cttcgccgaa
     1921 aaagagggga gttcaaaccg gttatattcg tacgctggag ctcgccctcg cctggatgtt
     1981 tgaaaatgtc gcgcgttccg aagatgcctt gcataacctc ctcgtccgtg acgccggaca
     2041 aggatcagct ctgctcgttg gtaaagattc gccggctgcc gagcgactcc atgcccgttg
     2101 ggctactagc cgtgtcaata agagcattac ccgcctcctc cgtcagttgg agctccctcc
     2161 taccgccacg gctacggcct cgataatgcc gcacgtgatg gagcagcctc tcagtaccag
     2221 cattaacccc gtcaacgacc gcttcaacgg tattcccaac cccactccgt ataactccga
     2281 tgcagctctc gatgctatca ctcagaccaa cgattatgga agcgtaaata cacatggtat
     2341 cctctctact tacccgccac cggctacgca ccttaatgaa gcttccgtcg ctctcgctcc
     2401 cggtggcgcc cccccccgac cgcctcctcc gtatgttgac agcacgacca atcacccgcc
     2461 gtaccactcg aatctggttc caatggcgaa ctttggttac tcgaccgttg attacgatgc
     2521 catggttgac gatttggcta gcattgaata cacggacgct gtggatgtcg acccacagtt
     2581 tatgaccaat ctgggattcg ttcctggatg taacttctcc gacattaata catacgaaca
     2641 gtgatgacgt ctatcgatac cgtcgactaa agccaaatag aaaattattc agttcctggc
     2701 ttaagttttt aaaagtgata ttatttattt ggttgtaacc aaccaaaaga atgtaaataa
     2761 ctaatacata attatgttag ttttaagtta gcaacaaatt gattttagct atattagcta
     2821 cttggttaat aaatagaata tatttattta aagataattg cgtttttatt gtcagggagt
     2881 gagtttgctt aaaaactcgt ttagatccac cgcatgcata acttcgtata gcatacatta
     2941 tacgaagtta tggtaccact cgacgattaa ccgttagttt agcaagtata attgaatttt
     3001 ttataagatc cagctaagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3061 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3121 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3181 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     3241 tcagagtcgc ggcgcttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc
     3301 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
     3361 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
     3421 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
     3481 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggttttctt
     3541 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
     3601 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
     3661 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
     3721 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
     3781 ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     3841 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
     3901 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
     3961 caccatgggc cgattgttta gcttgttcag ctgcgcttgt ttatttgctt agctttcgct
     4021 tagcgacgtg ttcactttgc ttgtttgaat tgaattgtcg ctccgtagac gaagcgcctc
     4081 tatttatact ccggcgctaa ttgaattagc tctaattgaa ttagtctcta attgaatgac
     4141 gtcataactt cgtatagcat acattatacg aagttataga tcactagtca tggcatcatt
     4201 gaaacagcaa ggctcttaca agttttcaca aaaatctatg aactagacaa aacagtaact
     4261 gcagaaaaaa gtcctatatg tgcaaaaaaa tgcttgatga tttgtaatga gattgaggag
     4321 gtttcgagac aggcaccaaa gtttttacaa atggatattt ccaccaccgc tctaaccaat
     4381 ttgttgaagg aacacccttg gctatccttt acaagattcg aactgaagtg gaaacagttg
     4441 tctcttatca tttatgtatt aagagatttt ttcactaatt ttacccagaa aaagtcacaa
     4501 ctagaacagg atcaaaatga tcatcaaagt tatgaagtta aacgatgctc catcatgtta
     4561 agcgatgcag cacaaagaac tgttatgtct gtaagtagct atatggacaa tcataatgtc
     4621 accccatatt ttgcctggaa ttgttcttat tacttgttca atgcagtcct agtacccata
     4681 aagactctac tctcaaactc aaaatcgaat gctgagaata acgagaccgc acaattatta
     4741 caacaaatta acactgttct gatgctatta aaaaaactgg ccacttttaa aatccagact
     4801 tgtgaaaaat acattcaagt actggaagag gtatgtgcgc cgtttctgtt atcacagtgt
     4861 gcaatcccat taccgcatat cagttataac aatagtaatg gtagcgccat taaaaatatt
     4921 gtcggttctg caactatcgc ccaataccct actcttccgg aggaaaatgt caacaatatc
     4981 agtgttaaat atgtttctcc tggctcagta gggccttcac ctgtgccatt gaaatcagga
     5041 gcaagtttca gtgatctagt caagctgtta tctaaccgtc caccctctcg taactctcca
     5101 gtgacaatac caagaagcac accttcgcat cgctcagtca cgccttttct agggcaacag
     5161 caacagctgc aatcattagt gccactgacc ccgtctgctt tgtttggtgg cgccaatttt
     5221 aatcaaagtg ggaatattgc tgatagctca ttgtccttca ctttcactaa cagtagcaac
     5281 ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc
     5341 tctaacgttc atgataactt catgaataat gaaatcacgg ctagtaaaat tgatgatggt
     5401 aataattcaa aaccactgtc acctggttgg acggaccaaa ctgcgtataa cgcgtttgga
     5461 atcactacag ggatgtttaa taccactaca atggatgatg tatataacta tctattcgat
     5521 gatgaagata ccccaccaaa cccaaaaaaa gagtaataga ctagtgaagt tcctattctc
     5581 tagaaagtat aggaacttct agtgatctga attcattttc aacgtcctcg atagtatagt
     5641 ggttagtatc cccgcctgtc acgcgggaga ccggggttca attccccgtc ggggagaatc
     5701 tgtgattctt tttttttttc ttttactttg ttatataaac aatttttgtt ttaattgaat
     5761 ctaatttgcc attgctttta ggaatctcag gcatccagca agcgtttgtc cgccgaatcg
     5821 cccatcagtg aagaagatcc tgtggcggct acgaaaatct ccccggccat gtcggcctcc
     5881 acctccagcg aaaaacccat cagcgagctg gccacctctg tgctgaccca ccgctttcca
     5941 gactccacct cctcacccgg cgaacatggc cttggacgaa tgcagttgtc gatccgctac
     6001 agcgcccagc gtcaaaaact agacgtgacc atacacaaaa tccagaagat accacttcgc
     6061 gatcccagca atatccccga tccgtatgtt aagctgtatc tgttgcctgg acgcaccaag
     6121 gagtcgaaac gcaagacgag cgtgatcaag gacaactgca accccgtcta cgatgcatcc
     6181 tttgagtacc tgatttccat tgccgaactc aggcagacgg aactggaggt gacggtgtgc
     6241 acccaaaagg gattcctatc cggcggtagt cccatcattg gcatggtagg tacccgaaag
     6301 caacccctta gttacagaca cagcgcgtac gtccttcgca tccttatgat tcccaagtac
     6361 atattctgca agagtacagt atatatagga aagatatccg ggtgaacttc gatgtgcagc
     6421 gtaccacaac gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     6481 ttgaaaaagt ggcaccgagt cggtgctttt ttgctcacct gtgattgctc ctactcaaat
     6541 acaaaaacat caaattttct gtcaataaag catatttatt tatatttatt ttacaggaaa
     6601 gaattccttt taaagtgtat tttaacctat aatgaaaaac gattaaaaaa aatacataaa
     6661 ataattcgaa aatttttgaa tagcccaggt tgataaaaat tcatttcata cgttttataa
     6721 cttatgcccc taagtatttt ttgaccatag tgtttcaatt ctacattaat tttacagagt
     6781 agaatgaaac gccacctact cagccaagag gcgaaaaggt tagctcgcca agcagagagg
     6841 gcgccagtgc tcactacttt ttataattct caacttcttt ttccagactc agttcgtata
     6901 tatagaccta ttttcaattt aacgtcgtgt attctgagaa agctggagtt ttagagctag
     6961 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     7021 tgcttttttg cctacctgga gcctgagagt tgttcaataa aataaaaatg tttcgttttt
     7081 ttgctttcgc cagtatttat tatttttcat caatatgtat tcaatttggt atgtatttag
     7141 taattgtaat atatagacaa tggttttccg ttgacgtaca tacatctgac gtgtgtttat
     7201 ttagacataa tagttatgtt ttcacatctt tttaatgttc gcttaatgcg tatgcttaat
     7261 taattctgac gtaagctagt tacaaacccc acggacatgc taagggttaa tcaacaatca
     7321 tatcgctgtc tcactcagac tcaatacgac actcagaata ctattccttt cactcgcact
     7381 tattgcaagc atacgttaag tggatgtctc ttgccgacgg gaccacctta tgttatttca
     7441 tcatgaaaat gaagtgaagt tcctatactt tctagagaat aggaacttct atagtgagtc
     7501 gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag
     7561 tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt
     7621 ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta
     7681 tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa
     7741 aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc
     7801 tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa
     7861 actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt
     7921 actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac
     7981 agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat
     8041 gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt
     8101 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8161 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8221 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8281 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8341 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8401 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8461 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8521 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8581 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8641 tacagagttc ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat
     8701 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8761 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8821 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8881 cgcgcgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca
     8941 gcgtaatgct ctgcttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9001 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9061 ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc ggctccggat
     9121 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9181 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9241 aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt
     9301 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9361 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9421 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9481 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9541 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9601 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9661 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9721 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9781 ggaataaggg cgacacggaa atgttgaata ctcatattct tcctttttca atattattga
     9841 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9901 aaacaaatag gggtcagtgt tacaaccaat taaccaattc tgaacattat cgcgagccca
     9961 tttatacctg aatatggctc ataacacccc ttgtttgcct ggcggcagta gcgcggtggt
    10021 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
    10081 gactccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
    10141 aagactgggc ctttcgccc
//
