LOCUS       sequence-205211-        6636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac vector for cloning 15xQUAS transgenes; used for mosquito 
            tranformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Riabinina O, Task D, Marr E, Lin CC, Alford R, O'Brochta DA, Potter 
            CJ
  TITLE     Organization of olfactory centres in the malaria mosquito Anopheles 
            gambiae.
  JOURNAL   Nat Commun. 2016 Oct 3;7:13010. doi: 10.1038/ncomms13010.
  PUBMED    27694947
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-3"; volume: "7:13010. doi"; pages: " 
            10.1038/ncomms13010"
COMMENT     Sequence Label: pBAC-ECFP-15xQUAS_TATA-SV40
FEATURES             Location/Qualifiers
     source          1..6636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    225..359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   634..668
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(1159..1175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1159..1175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1172..1194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1183..1199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1207..1213,1214..1231,1232..1237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1252..1273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1390..1407)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1561..2149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1641..1660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2320..3111,3112..3180))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2943..2962
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3181..3285)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3311..3766)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3448..3469)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3660..3679
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3893..3915
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3907..3924
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3908..3924
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   4054..4116
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      4602..4611
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4608..4610,4611..4613,4614..5327)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4653..4674)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4914..4933)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5261..5282
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5450..5571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5983..6221
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6168..6189
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          6374..6439
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6569..6589
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
       61 tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga
      121 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      181 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
      241 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      301 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg
      361 atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
      421 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
      481 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
      541 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
      601 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
      661 ttctagggtt aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc
      721 atcgcccaag ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg
      781 aggttgaagc ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc
      841 gaaaacgcac gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg
      901 aactgttcgt tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc
      961 ggatggtcag cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc
     1021 gtgccggtgt gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg
     1081 ggtatcctgg ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc
     1141 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1201 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1261 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1321 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1381 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1981 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2221 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2281 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2341 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2401 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2461 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2521 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2581 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2641 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2701 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2761 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2821 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2881 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2941 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3001 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3061 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3121 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3181 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3241 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3301 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3361 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3421 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3481 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3541 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3601 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3661 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3721 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     3781 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3841 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3901 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
     3961 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
     4021 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
     4081 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
     4141 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     4201 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     4261 aatttcttct ataaagtaac aaaactttta tcgaattcct gcagcccggg ggatccacta
     4321 gttctagtgt tcccacaatg gttaattcga gctcgcccgg ggatctaatt caattagaga
     4381 ctaattcaat tagagctaat tcaattagga tccaagctta tcgatttcga accctcgacc
     4441 gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa
     4501 gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac
     4561 aatcggggta ccgctagagt cgacggtacg atccaccggt cgccaccatg gtgagcaagg
     4621 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4681 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4741 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4801 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4861 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4921 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4981 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5041 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     5101 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     5161 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     5221 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5281 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact
     5341 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5401 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5461 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5521 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaagcttatc
     5581 gatacgcgta cggcactaga gcggccgcca ccgcggtcac gtgtcactgg gtcagtgggt
     5641 aatcgcttat cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa
     5701 tcgcttatcc tcgggtaatc gcttatcctt gggtaatcgc ttatcctcgg ataaacaatt
     5761 atcctcacgg gtaatcgctt atccgctcgg gtaatcgctt atcctcgggt aatcgcttat
     5821 ccttgggtaa tcgcttatcc tcggataaac aattatcctc acgggtaatc gcttatccgc
     5881 tcgggtaatc gcttatcctc gggtaatcgc ttatcctttc acgttgggac tcagtgagga
     5941 ggacctgaat tcctgcagcc cgagcggaga ctctagcgag cgccggagta taaatagagg
     6001 cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg
     6061 aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa
     6121 gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa
     6181 tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa ttgggaattc
     6241 gttaacagat cgggggatca attcgttaac agatctgcgg ccgcggctcg agacgtcgat
     6301 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     6361 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     6421 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     6481 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     6541 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     6601 ggactttcct tcagaattgc taagtttttt gagtca
//
