LOCUS       sequence-205214-        6490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  General purpose vector for cloning enhancer/promoter regions to 
            drive QF2 expression; used for mosquito.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Riabinina O, Task D, Marr E, Lin CC, Alford R, O'Brochta DA, Potter 
            CJ
  TITLE     Organization of olfactory centres in the malaria mosquito Anopheles 
            gambiae.
  JOURNAL   Nat Commun. 2016 Oct 3;7:13010. doi: 10.1038/ncomms13010.
  PUBMED    27694947
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-3"; volume: "7:13010. doi"; pages: " 
            10.1038/ncomms13010"
COMMENT     Sequence Label: pBAC-DsRed-QF2-hsp70
FEATURES             Location/Qualifiers
     source          1..6490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   147..209
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1371
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(874..894)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1286..1309
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1492..1573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1529..1548)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1583..1602
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3217..3251
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3742..3758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3742..3758)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3755..3777)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3766..3782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3790..3796,3797..3814,3815..3820))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3835..3856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3973..3990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4144..4732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4224..4243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4903..5694,5695..5763))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5526..5545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5764..5868)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5894..6349)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6031..6052)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6243..6262
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(6476..6490,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(6490,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg
       61 ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca
      121 cgctgcagaa cacgcagcta gattaaccct agaaagataa tcatattgtg acgtacgtta
      181 aagataatca tgtgtaaaat tgacgcatgt gttttatcgg tctgtatatc gaggtttatt
      241 tattaatttg aatagatatt aagttttatt atatttacac ttacatacta ataataaatt
      301 caacaaacaa tttatttatg tttatttatt tattaaaaaa aacaaaaact caaaatttct
      361 tctataaagt aacaaaactt ttatcgaatt cctgcagccc gggggatcca ctagttctag
      421 tgttcccaca atggttaatt cgagctcgcc cggggatcta attcaattag agactaattc
      481 aattagagct aattcaatta ggatccaagc ttatcgattt cgaaccctcg accgccggag
      541 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
      601 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatcggg
      661 gtaccgcggg cccgggatcc accggtcgcc accatggcct cctccgagaa cgtcatcacc
      721 gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc
      781 gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacc
      841 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
      901 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
      961 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac cgtgacccag
     1021 gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc
     1081 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc caccgagcgc
     1141 ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa gctgaaggac
     1201 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg
     1261 cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga ggactacacc
     1321 atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgta gcggccgcga
     1381 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     1441 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     1501 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     1561 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc tcgaggccgg
     1621 ccaacatgcc acccaagcgc aaaacgctta acgctgcggc tgaggctaac gctcatgccg
     1681 acggacacgc cgacggaaac gccgacggac acgtggccaa tacggccgcg tcctcgaata
     1741 atgcgaggtt cgctgatctc actaacatcg atactccggg tctgggaccc acaactacga
     1801 ccctgctcgt ggaaccagca cgctcaaagc gtcaacgagt gtcccgcgca tgcgaccagt
     1861 gccgtgcagc ccgagagaaa tgcgacggaa tacagcctgc gtgtttcccg tgcgtttccc
     1921 agggaaggtc ctgcacttat caggcttcgc cgaaaaagag gggagttcaa accggttata
     1981 ttcgtacgct ggagctcgcc ctcgcctgga tgtttgaaaa tgtcgcgcgt tccgaagatg
     2041 ccttgcataa cctcctcgtc cgtgacgccg gacaaggatc agctctgctc gttggtaaag
     2101 attcgccggc tgccgagcga ctccatgccc gttgggctac tagccgtgtc aataagagca
     2161 ttacccgcct cctccgtcag ttggagctcc ctcctaccgc cacggctacg gcctcgataa
     2221 tgccgcacgt gatggagcag cctctcagta ccagcattaa ccccgtcaac gaccgcttca
     2281 acggtattcc caaccccact ccgtataact ccgatgcagc tctcgatgct atcactcaga
     2341 ccaacgatta tggaagcgta aatacacatg gtatcctctc tacttacccg ccaccggcta
     2401 cgcaccttaa tgaagcttcc gtcgctctcg ctcccggtgg cgcccccccc cgaccgcctc
     2461 ctccgtatgt tgacagcacg accaatcacc cgccgtacca ctcgaatctg gttccaatgg
     2521 cgaactttgg ttactcgacc gttgattacg atgccatggt tgacgatttg gctagcattg
     2581 aatacacgga cgctgtggat gtcgacccac agtttatgac caatctggga ttcgttcctg
     2641 gatgtaactt ctccgacatt aatacatacg aacagtgatg acgtctatcg ataccgtcga
     2701 ctaaagccaa atagaaaatt attcagttcc tggcttaagt ttttaaaagt gatattattt
     2761 atttggttgt aaccaaccaa aagaatgtaa ataactaata cataattatg ttagttttaa
     2821 gttagcaaca aattgatttt agctatatta gctacttggt taataaatag aatatattta
     2881 tttaaagata attgcgtttt tattgtcagg gagtgagttt gcttaaaaac tcgtttagat
     2941 ccgatatcta taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa
     3001 taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt
     3061 cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc
     3121 acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg
     3181 cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta
     3241 tctttctagg gttaatctag ctgcatcagg atcatatcgt cgggtctttt ttccggctca
     3301 gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc
     3361 gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg
     3421 agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg
     3481 gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt
     3541 tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact
     3601 gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg
     3661 acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg
     3721 ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3781 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3841 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3901 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3961 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4021 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4081 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4141 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4201 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4261 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4321 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4381 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4441 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4501 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4561 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4621 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4681 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4741 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4801 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4861 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4921 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4981 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5041 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5101 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5161 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5221 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5281 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5341 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5401 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5461 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5521 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5581 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5641 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5701 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5761 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5821 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5881 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     5941 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     6001 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     6061 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     6121 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     6181 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6241 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6301 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     6361 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     6421 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     6481 tcacgacgtt
//
