LOCUS       sequence-20522-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(121..245)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(322..343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(583..602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cggggaagaa gtggctgata ctcagccacc gcgaaaatga catcaaaaac gccattaacc
       61 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
      121 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
      181 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
      241 gtggtgatgg gctgcaggaa ttcgatatca agcttaatgg tgagcaaggg cgaggagctg
      301 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      361 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      421 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      481 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      541 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      601 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      661 atcgac
//
