LOCUS       sequence-205221-        8204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of vsv-INCENP 8KQ .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   van der Horst A, Vromans MJ, Bouwman K, van der Waal MS, Hadders MA,
            Lens SM
  TITLE     Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from
            Centromeres to Microtubules.
  JOURNAL   Cell Rep. 2015 Jul 21;12(3):380-7. doi: 
            10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9.
  PUBMED    26166576
REFERENCE   2  (bases 1 to 8204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.038"; journalName: "Cell Rep"; date: 
            "2015-07-21- 21"; volume: "12"; issue: "3"; pages: "380-7"
COMMENT     Sequence Label: pCR3-vsv-Incenp 8KQ
FEATURES             Location/Qualifiers
     source          1..8204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             932..964
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     primer_bind     complement(3798..3815)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3804..4028
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4074..4502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4161..4180)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4371..4392
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4511..4531)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4516..4845
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4696..4831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4758..4777
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4912..5706
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4966..4985)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5576..5595
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5880..6001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5917..5936)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5971..5990
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6050..6066)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6050..6066)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6063..6085)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6074..6090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6098..6104,6105..6122,6123..6128))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6143..6164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6281..6298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6452..7037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6532..6551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7208..7999,8000..8068))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7831..7850
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8069..8173)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagtactccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgccaccat gtacaccgac atcgagatga accggttggg
      961 caaggggacg acggccccag ggcccattca cctgctggag ctctgtgacc agaagctcat
     1021 ggagtttctc tgcaacatgg ataataagga cttggtgtgg cttgaggaga tccaagagga
     1081 ggccgagcgc atgttcacca gagaattcag caaagagcca gagctgatgc ccaaaacacc
     1141 ttctcagaag aaccgacgga agaagagacg gatttcttat gttcaggatg aaaacagaga
     1201 tcccatcagg agaaggttat cccgcagaaa gtctcggagc agccagcaga gcacccgacg
     1261 cctccgcagc aaggacagtg tagagaagct ggctacagtg gtcggggaga acggctccgt
     1321 tctgcggcgt gtgacccgtg ctgcggctgc agctgccgcg gctaccatgg cattggctgc
     1381 accttcttca cccacccctg agtctcccac gatgctgact aagaagcccg aggataacca
     1441 cacccagtgc cagctggtgc ctgtggtgga gatcggcatc agtgagcgcc agaatgctga
     1501 gcagcatgtc acccagctca tgtccaccga gcctctgccc cgcactctgt ccccgactcc
     1561 agcttcagcc acagctccaa cctcccaggg catcccgaca tcagatgagg aatcaacacc
     1621 taagaagtcg aaggccagga tactggagtc catcacagtg agctccctga tggctacacc
     1681 ccaggacccc aagggtcaag gggtcgggac ggggcggtct gcgtctaagc tcaggattgc
     1741 gcaggtctcc cctggcccac gggactcgcc agcctttcca gattctccat ggcgggagcg
     1801 ggtgctggct cccatcctgc cggataactt ctccacgccc acgggctctc gcacggactc
     1861 tcaatcggtg cggcacagcc cgatcgcccc gtcttccccg agtccccgag tcttagccca
     1921 gaagtactct ctggtggcca aacaggaaag tgttgtccgc agggcgagca gaaggcttgc
     1981 caagaagact gccgaagagc cagctgcctc tggccgcatc atctgtcaca gttacctgga
     2041 gaggctcctg aatgttgagg tgccccagaa agttggttct gagcagaagg aaccccccga
     2101 ggaggctgag cctgtggcgg cagctgagcc agaggtccct gagaacaacg gaaataactc
     2161 gtggccccac aatgacacag agatcgccaa cagcacaccc aacccgaagc ctgcagccag
     2221 cagcccggaa acaccctctg cagggcagca agaggccaag acggaccaag cagatggacc
     2281 cagagagcca ccgcagagtg ccaggaggaa gcgcagctac aagcaggccg tgagtgagct
     2341 ggacgaggag cagcacctgg aggatgagga gctgcagccc cccaggagca agaccccttc
     2401 ctcaccctgc ccagccagca aggtggtacg gcccctccgg acctttctgc acacagtgca
     2461 gaggaaccag atgctcacga ccccgacctc agccccacgc agcgtcatga agtcctttat
     2521 taagcgcaac actcccctgc gcatggaccc caagtgcagc ttcgtcgaga aggagcggca
     2581 gcgcctggag cttaagagat ctgaaagcag caatctgcgg cggcaggagg aggccgagca
     2641 gctgcgcagg cagaaggtgg aggaggacca gcggcggcgg ctggaggagg tgaagctgaa
     2701 gcgtgaggaa cgcctccgca aggtgctgca ggcccgcgag cgggtggagc agatgaagga
     2761 ggagaagaag aagcagattg agcagcagtt tgctcagatc gacgagcaga ctgagaaggc
     2821 ccaggaggag cggctggcag aggagcaggc caagaaaaag gcggcggccc agcagatgga
     2881 ggaggtggaa gcacgcagga agcaggaaga ggatgcacgt aggctcaggt ggctgcagca
     2941 ggaggaggaa gagcggcggc accaagagct gctgcagaag aagaaggaag aggagcagga
     3001 gcggctgcgg aaggcggccg aggctaagcg gctggcagag cagcgggagc aggagcggcg
     3061 ggagcaggag cggcgcgagc aggagcggcg cgagcaggag cggcgggagc aggagcggcg
     3121 cgagcaggag cgacagctgg cagagcagga gcgtcggcgg gagcaggagc ggctccaggc
     3181 cgagagggag ctgcaggagc gggagaaggc cctgcggctg ggaagcagcg gatcccttaa
     3241 ggagcagctg cagagggaac tggaggagaa gaagaagaag gaagagcagc agcgtctggc
     3301 tgagcggcag ctgcaggagg agcaagagaa gaaagccaag gaggcagtag gggccagcaa
     3361 ggccctgaat gtgactgtgg acgtgcagtc tccagcttgt acctcatatc aaatgactcc
     3421 gcaagggcac agggcccctc ccaagatcaa cccagataac tacgggatgg atctgaatag
     3481 cgacgactcc accgatgatg aggcccatcc ccggaagccc atccccacct gggcccgagg
     3541 caccccgctc agccaggcta tcattcacca gtactaccac ccaccgaacc ttctggagct
     3601 ctttggaacc attctcccac tggacttgga ggatatcttc aagaagagca agccccgcta
     3661 tcacaagcgc accagctctg ctgtctggaa ctcaccgccc ctgcagggcg ccagggtccc
     3721 cagcagcctg gcctacagcc tgaagaagca caagctttga ctcgagtcta gagggcccgt
     3781 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3841 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3901 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3961 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4021 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4081 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4141 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4201 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4261 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4321 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4381 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4441 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4501 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4561 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4621 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4681 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4741 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4801 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4861 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     4921 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4981 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5041 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5101 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5161 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5221 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5281 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5341 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5401 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5461 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5521 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5581 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5641 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5701 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5761 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5821 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5881 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5941 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6001 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6061 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6121 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6181 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6241 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6961 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     7021 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7081 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7141 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7201 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7261 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7321 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7381 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7441 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7501 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7561 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7621 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7681 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7741 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7801 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7861 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7921 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7981 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8041 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8101 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8161 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
