LOCUS       sequence-205225-        8720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of vsv-INCENP 8KR-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   van der Horst A, Vromans MJ, Bouwman K, van der Waal MS, Hadders MA,
            Lens SM
  TITLE     Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from
            Centromeres to Microtubules.
  JOURNAL   Cell Rep. 2015 Jul 21;12(3):380-7. doi: 
            10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9.
  PUBMED    26166576
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.038"; journalName: "Cell Rep"; date: 
            "2015-07-21- 21"; volume: "12"; issue: "3"; pages: "380-7"
COMMENT     Sequence Label: pcDNA5-FRT-TO-vsv-Incenp 8KR-GFP
FEATURES             Location/Qualifiers
     source          1..8720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1030..1062
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     regulatory      3901..3910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3907..3909,3910..3912,3913..4626)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3907..3909,3910..3912,3913..4623)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3952..3973)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4213..4232)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4560..4581
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4672..4689)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4678..4902
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5035..5054)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5186..5233
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5241..6263
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5241..6263
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6393..6514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6430..6449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6484..6503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6563..6579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6563..6579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6576..6598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6587..6603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6611..6617,6618..6635,6636..6641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6656..6677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6794..6811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6965..7553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7045..7064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7724..8515,8516..8584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8347..8366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8585..8689)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc tcgagctcaa gcttggtacc
     1021 gccaccatgt acaccgacat cgagatgaac cggttgggca aggggacgac ggccccaggg
     1081 cccattcacc tgctggagct ctgtgaccag aagctcatgg agtttctctg caacatggat
     1141 aataaggact tggtgtggct tgaggagatc caagaggagg ccgagcgcat gttcaccaga
     1201 gaattcagca aagagccaga gctgatgccc aaaacacctt ctcagaagaa ccgacggaag
     1261 aagagacgga tttcttatgt tcaggatgaa aacagagatc ccatcaggag aaggttatcc
     1321 cgcagaaagt ctcggagcag ccagcagagc acccgacgcc tccgcagcaa ggacagtgta
     1381 gagaagctgg ctacagtggt cggggagaac ggctccgttc tgcggcgtgt gacccgtgct
     1441 gcggctgcag ctgccgcggc taccatggca ttggctgcac cttcttcacc cacccctgag
     1501 tctcccacga tgctgactaa gaagcccgag gataaccaca cccagtgcca gctggtgcct
     1561 gtggtggaga tcggcatcag tgagcgccag aatgctgagc agcatgtcac ccagctcatg
     1621 tccaccgagc ctctgccccg cactctgtcc ccgactccag cttcagccac agctccaacc
     1681 tcccagggca tcccgacatc agatgaggaa tcaacaccta agaagtcgaa ggccaggata
     1741 ctggagtcca tcacagtgag ctccctgatg gctacacccc aggaccccaa gggtcaaggg
     1801 gtcgggacgg ggcggtctgc gtctaagctc aggattgcgc aggtctcccc tggcccacgg
     1861 gactcgccag cctttccaga ttctccatgg cgggagcggg tgctggctcc catcctgccg
     1921 gataacttct ccacgcccac gggctctcgc acggactctc aatcggtgcg gcacagcccg
     1981 atcgccccgt cttccccgag tccccgagtc ttagcccaga agtactctct ggtggccaaa
     2041 caggaaagtg ttgtccgcag ggcgagcaga aggcttgcca agaagactgc cgaagagcca
     2101 gctgcctctg gccgcatcat ctgtcacagt tacctggaga ggctcctgaa tgttgaggtg
     2161 ccccagaaag ttggttctga gcagaaggaa ccccccgagg aggctgagcc tgtggcggca
     2221 gctgagccag aggtccctga gaacaacgga aataactcgt ggccccacaa tgacacagag
     2281 atcgccaaca gcacacccaa cccgaagcct gcagccagca gcccggaaac accctctgca
     2341 gggcagcaag aggccaagac ggaccaagca gatggaccca gagagccacc gcagagtgcc
     2401 aggaggaagc gcagctacaa gcaggccgtg agtgagctgg acgaggagca gcacctggag
     2461 gatgaggagc tgcagccccc caggagcaag accccttcct caccctgccc agccagcaag
     2521 gtggtacggc ccctccggac ctttctgcac acagtgcaga ggaaccagat gctcacgacc
     2581 ccgacctcag ccccacgcag cgtcatgaag tcctttatta agcgcaacac tcccctgcgc
     2641 atggacccca agtgcagctt cgtcgagaag gagcggcagc gcctggagct taagagatct
     2701 gaaagcagca atctgcggcg gagggaggag gccgagcagc tgcgcaggca gaaggtggag
     2761 gaggacaggc ggcggcggct ggaggaggtg aagctgaagc gtgaggaacg cctccgcaag
     2821 gtgctgcagg cccgcgagcg ggtggagcag atgaaggagg agaagaagaa gcagattgag
     2881 cagaggtttg ctcagatcga cgagaggact gagaaggcca gggaggagcg gctggcagag
     2941 gagagggcca agaaaaaggc ggcggccagg aggatggagg aggtggaagc acgcaggaag
     3001 caggaagagg atgcacgtag gctcaggtgg ctgcagcagg aggaggaaga gcggcggcac
     3061 caagagctgc tgcagaagaa gaaggaagag gagcaggagc ggctgcggaa ggcggccgag
     3121 gctaagcggc tggcagagca gcgggagcag gagcggcggg agcaggagcg gcgcgagcag
     3181 gagcggcgcg agcaggagcg gcgggagcag gagcggcgcg agcaggagcg acagctggca
     3241 gagcaggagc gtcggcggga gcaggagcgg ctccaggccg agagggagct gcaggagcgg
     3301 gagaaggccc tgcggctggg aagcagcgga tcccttaagg agcagctgca gagggaactg
     3361 gaggagaaga agaagaagga agagcagcag cgtctggctg agcggcagct gcaggaggag
     3421 caagagaaga aagccaagga ggcagtaggg gccagcaagg ccctgaatgt gactgtggac
     3481 gtgcagtctc cagcttgtac ctcatatcaa atgactccgc aagggcacag ggcccctccc
     3541 aagatcaacc cagataacta cgggatggat ctgaatagcg acgactccac cgatgatgag
     3601 gcccatcccc ggaagcccat ccccacctgg gcccgaggca ccccgctcag ccaggctatc
     3661 attcaccagt actaccaccc accgaacctt ctggagctct ttggaaccat tctcccactg
     3721 gacttggagg atatcttcaa gaagagcaag ccccgctatc acaagcgcac cagctctgct
     3781 gtctggaact caccgcccct gcagggcgcc agggtcccca gcagcctggc ctacagcctg
     3841 aagaagcaca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc
     3901 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3961 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4021 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4081 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     4141 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     4201 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4261 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4321 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     4381 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4441 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4501 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4561 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     4621 aagtaaagcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     4681 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     4741 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     4801 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4861 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4921 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4981 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5041 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5101 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5161 attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa
     5221 gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     5281 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     5341 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     5401 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     5461 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     5521 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     5581 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     5641 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     5701 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     5761 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     5821 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     5881 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     5941 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     6001 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     6061 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     6121 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     6181 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     6241 ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct
     6301 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6361 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     6421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6481 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6541 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6601 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6661 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6721 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7381 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8701 gaaaagtgcc acctgacgtc
//
