LOCUS       sequence-205256-        9111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of vsv-Mis12-dCEN-INCENP-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM
  TITLE     Sensing chromosome bi-orientation by spatial separation of aurora B 
            kinase from kinetochore substrates.
  JOURNAL   Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. 
            Epub 2009 Jan 15.
  PUBMED    19150808
REFERENCE   2  (bases 1 to 9111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1167000"; journalName: "Science"; date: "2009-03-6-
            6"; volume: "323"; issue: "5919"; pages: "1350-3"
COMMENT     Sequence Label: pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFP
FEATURES             Location/Qualifiers
     source          1..9111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1036..1068
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     regulatory      4351..4360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4357..4359,4360..4362,4363..5076)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4357..4359,4360..4362,4363..5073)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4402..4423)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4663..4682)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5010..5031
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5122..5139)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5128..5352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5398..5826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5485..5504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5695..5716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5835..5855)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5840..6169
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6020..6155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6082..6101
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6217..6264
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6283..6657
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6787..6908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6824..6843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6878..6897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6957..6973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6957..6973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6970..6992)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6981..6997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7005..7011,7012..7029,7030..7035))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7050..7071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7188..7205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7359..7944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7439..7458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8115..8906,8907..8975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8738..8757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8976..9080)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc tcgagctcaa gcttggtacc
     1021 ggatccgcca ccatgtacac cgacatcgag atgaaccggt tgggcaagga attctctgtg
     1081 gatccaatga cctacgaggc ccagttcttt ggcttcacgc cacaaacgtg catgcttcgg
     1141 atctacattg catttcaaga ctacctattt gaagtgatgc aggccgttga acaggttatt
     1201 ctgaagaagc tggatggcat cccagactgt gacattagcc cagtgcagat tcgcaaatgc
     1261 acagagaagt ttctttgctt catgaaagga cattttgata acctttttag caaaatggag
     1321 caactgtttt tgcagctgat tttacgtatt ccctcaaaca tcttgcttcc tgaagataaa
     1381 tgtaaggaga caccttatag tgaggaagat tttcagcatc tccagaaaga aattgaacag
     1441 ttacaggaga agtacaagac tgaattatgt actaagcagg cccttcttgc agaattagaa
     1501 gagcaaaaaa ttgttcaggc caaactcaaa cagacgttga ctttctttga tgagcttcat
     1561 aatgttggca gagatcatgg gactagtgat tttagggaga gtttagtatc cctggttcag
     1621 aactccagaa aactacagaa cattagagac aatgtggaaa aggaatcgaa acgactgaaa
     1681 atatctgaat tcagcaaaga gccagagctg atgcccaaaa caccttctca gaagaaccga
     1741 cggaagaaga gacggatttc ttatgttcag gatgaaaaca gagatcccat caggagaagg
     1801 ttatcccgca gaaagtctcg gagcagccag cagagcaccc gacgcctccg cagcaaggac
     1861 agtgtagaga agctggctac agtggtcggg gagaacggct ccgttctgcg gcgtgtgacc
     1921 cgtgctgcgg ctgcagctgc cgcggctacc atggcattgg ctgcaccttc ttcacccacc
     1981 cctgagtctc ccacgatgct gactaagaag cccgaggata accacaccca gtgccagctg
     2041 gtgcctgtgg tggagatcgg catcagtgag cgccagaatg ctgagcagca tgtcacccag
     2101 ctcatgtcca ccgagcctct gccccgcact ctgtccccga ctccagcttc agccacagct
     2161 ccaacctccc agggcatccc gacatcagat gaggaatcaa cacctaagaa gtcgaaggcc
     2221 aggatactgg agtccatcac agtgagctcc ctgatggcta caccccagga ccccaagggt
     2281 caaggggtcg ggacggggcg gtctgcgtct aagctcagga ttgcgcaggt ctcccctggc
     2341 ccacgggact cgccagcctt tccagattct ccatggcggg agcgggtgct ggctcccatc
     2401 ctgccggata acttctccac gcccacgggc tctcgcacgg actctcaatc ggtgcggcac
     2461 agcccgatcg ccccgtcttc cccgagtccc cgagtcttag cccagaagta ctctctggtg
     2521 gccaaacagg aaagtgttgt ccgcagggcg agcagaaggc ttgccaagaa gactgccgaa
     2581 gagccagctg cctctggccg catcatctgt cacagttacc tggagaggct cctgaatgtt
     2641 gaggtgcccc agaaagttgg ttctgagcag aaggaacccc ccgaggaggc tgagcctgtg
     2701 gcggcagctg agccagaggt ccctgagaac aacggaaata actcgtggcc ccacaatgac
     2761 acagagatcg ccaacagcac acccaacccg aagcctgcag ccagcagccc ggaaacaccc
     2821 tctgcagggc agcaagaggc caagacggac caagcagatg gacccagaga gccaccgcag
     2881 agtgccagga ggaagcgcag ctacaagcag gccgtgagtg agctggacga ggagcagcac
     2941 ctggaggatg aggagctgca gccccccagg agcaagaccc cttcctcacc ctgcccagcc
     3001 agcaaggtgg tacggcccct ccggaccttt ctgcacacag tgcagaggaa ccagatgctc
     3061 acgaccccga cctcagcccc acgcagcgtc atgaagtcct ttattaagcg caacactccc
     3121 ctgcgcatgg accccaagtg cagcttcgtc gagaaggagc ggcagcgcct ggagaatctg
     3181 cggcggaagg aggaggccga gcagctgcgc aggcagaagg tggaggagga caagcggcgg
     3241 cggctggagg aggtgaagct gaagcgtgag gaacgcctcc gcaaggtgct gcaggcccgc
     3301 gagcgggtgg agcagatgaa ggaggagaag aagaagcaga ttgagcagaa gtttgctcag
     3361 atcgacgaga agactgagaa ggccaaggag gagcggctgg cagaggagaa ggccaagaaa
     3421 aaggcggcgg ccaagaagat ggaggaggtg gaagcacgca ggaagcagga agaggatgca
     3481 cgtaggctca ggtggctgca gcaggaggag gaagagcggc ggcaccaaga gctgctgcag
     3541 aagaagaagg aagaggagca ggagcggctg cggaaggcgg ccgaggctaa gcggctggca
     3601 gagcagcggg agcaggagcg gcgggagcag gagcggcgcg agcaggagcg gcgcgagcag
     3661 gagcggcggg agcaggagcg gcgcgagcag gagcgacagc tggcagagca ggagcgtcgg
     3721 cgggagcagg agcggctcca ggccgagagg gagctgcagg agcgggagaa ggccctgcgg
     3781 ctgcagaagg agcagctgca gagggaactg gaggagaaga agaagaagga agagcagcag
     3841 cgtctggctg agcggcagct gcaggaggag caagagaaga aagccaagga ggcagtaggg
     3901 gccagcaagg ccctgaatgt gactgtggac gtgcagtctc cagcttgtac ctcatatcaa
     3961 atgactccgc aagggcacag ggcccctccc aagatcaacc cagataacta cgggatggat
     4021 ctgaatagcg acgactccac cgatgatgag gcccatcccc ggaagcccat ccccacctgg
     4081 gcccgaggca ccccgctcag ccaggctatc attcaccagt actaccaccc accgaacctt
     4141 ctggagctct ttggaaccat tctcccactg gacttggagg atatcttcaa gaagagcaag
     4201 ccccgctatc acaagcgcac cagctctgct gtctggaact caccgcccct gcagggcgcc
     4261 agggtcccca gcagcctggc ctacagcctg aagaagcaca agcttcgaat tctgcagtcg
     4321 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     4381 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     4441 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     4501 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     4561 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4621 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4681 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4741 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4801 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     4861 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4921 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     4981 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     5041 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgctcgag tctagagggc
     5101 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5161 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5221 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5281 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5341 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     5401 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5461 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5521 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5581 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5641 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5701 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5761 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5821 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6181 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     6241 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     6301 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     6361 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     6421 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     6481 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     6541 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     6601 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     6661 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     6721 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6781 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6841 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6901 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6961 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7021 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7081 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7141 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7861 tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt
     7921 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7981 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8041 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8101 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8161 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8221 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8281 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8341 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8401 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8461 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8521 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8581 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8641 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8701 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8761 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8821 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8881 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8941 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9001 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9061 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
