LOCUS       sequence-205259-        9225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synOptopatch construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE
  TITLE     All-optical synaptic electrophysiology probes mechanism of 
            ketamine-induced disinhibition.
  JOURNAL   Nat Methods. 2018 Oct;15(10):823-831. doi: 
            10.1038/s41592-018-0142-8. Epub 2018 Oct 1.
  PUBMED    30275587
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0142-8"; journalName: "Nat Methods"; date: 
            "2018-10"; volume: "15"; issue: "10"; pages: "823-831"
COMMENT     Sequence Label: LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark...
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     723..745
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     807..826
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    854..865
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             941..958
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             962..994
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(998..1006,1007..1021)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1049..1105
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     1801..1820
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3983..4324
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             4340..4396
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4397..5155
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAARERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             join(5225..5227,5228..5938)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGGGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5267..5288)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5528..5547)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5875..5896
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6243..6262)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6308..6363
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6362..6381)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6724..6740)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6724..6740)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6737..6759)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6748..6764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6772..6778,6779..6796,6797..6802))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6817..6838
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6897..7092
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6943..7078
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7098..7232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7135..7154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7189..7208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7300..7317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7471..8059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7551..7570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8230..9021,9022..9090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8853..8872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9091..9195)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
      481 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
      541 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
      601 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
      661 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
      721 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
      781 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
      841 tggcaaagaa ttcgtcatcc tcatcgccgc caccatgctg cagaacgagc ttgctcttaa
      901 gttggctgga cttgatatta acaagactgg aggaggttct catcatcatc atcatcatgg
      961 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
     1021 ggatctcgca acaatggtcg actcatcacg tcgtaagtgg aataaggcag gtcacgcagt
     1081 cagagctata ggtcggctga gctcacccgt ggtttccgag cggatgtacc ccgaggacgg
     1141 cgccctgaag agcgagatca agaaggggct gaggctgaag gacggcggcc actacgccgc
     1201 cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt
     1261 ggacatcaag ttggacatcg tgtcccacaa cgaggactac accatcgtgg aacagtgcga
     1321 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gaggtacagg
     1381 cgggagtctg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     1441 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     1501 cgagggccgc ccctacgagg cctttcagac cgctaagctg aaggtgacca agggtggccc
     1561 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacat
     1621 taagcaccca gccgacatcc ccgactactt caagctgtcc ttccccgagg gcttcaggtg
     1681 ggagcgcgtg atgaacttcg aggacggcgg cattattcac gtgaaccagg actcctccct
     1741 gcaggacggc gtattcatct acaaggtgaa gctgcgcggc accaacttcc cccccgacgg
     1801 ccccgtaatg cagaagaaga ccatgggctg ggaggctacg cgtgacgacc tgactgaaga
     1861 gcagatcgca gaatttaaag aggctttctc cctatttgac aaggacgggg atgggacgat
     1921 aacaaccaag gagcttggga cggtgttccg atcgctgggg cagaacccca cagaagcaga
     1981 gctgcaggac atgatcaatg aagtagatgc cgacggtgac ggcacattcg acttccctga
     2041 gttcctgacg atgatggcaa gaaaaatgaa tgacacagac agtgaagagg aaattcgcga
     2101 agcgttccgc gtgtttgata aggacggcaa tggctacatc ggcgcagcag agcttcgcca
     2161 cgtgatgaca gaccttggag agaagttaac agatgaggag gttgatgaaa tgatcagggt
     2221 agcagacatc gatggggatg gtcaggtaaa ctacgaagag tttgtacaaa tgatgacagc
     2281 gaagggtgga ggttctggtg gaggttctgg aggtggatca ggaggaggtt cgagatctaa
     2341 gctctcaggt ggcggaggga gcggcggagg tggctctgga ggtggtggct cagctgaagc
     2401 atggtacaat ctgggaaacg cttattacaa gcagggcgac tatcaaaaag ccattgagta
     2461 ttaccaaaag gctcttgagc tagaccctaa taacgccgaa gcgtggtata acctaggcaa
     2521 cgcatattac aagcagggag actaccaaaa ggcaatcgaa tattaccaga aggcactcga
     2581 actggaccca aataacgcag aggcttggta taatctggga aacgcctact ataaacaggg
     2641 tgactaccaa aaggcgatag aggactatca gaaggccttg gagctcgatc cgaacaatct
     2701 gcaacgtagt gctggtggcg gagggtctgg tggaggaggc tcaggaggag gtggcgcatc
     2761 aaacgttgcc aagtacgggc accttgaagc cagccctgat gatgggcaga atcagttcag
     2821 tgatatcatt aagtccagat ctgatgaaca caacgatgta cagaagaaaa cctttaccaa
     2881 atggataaac gctcgatttt caaagagtgg gaaaccaccc atcaatgata tgttctcaga
     2941 cctcaaagat gggagaaagc tcttggatct tctagaaggc ctcacaggaa catcattgcc
     3001 aaaggaacgt ggttccacaa gggtgcatgc cttaaataat gtcaaccggg tgctgcaggt
     3061 tttacatcag aacaatgtgg agctggtgaa tatcggaggc actgacattg tggatggaaa
     3121 tcccaagctg acgctgggtt tgctgtggag tattattctg cactggcagg tgaaggatgt
     3181 catgaaagat atcatgtcag acctgcagca gacgaacagc gagaagatcc tgctgagctg
     3241 ggtacggcag accaccaggc cctacagcca agtcaacgtc ctcaacttca ccaccagctg
     3301 gacagatgga ctcgcattca acgccgtgct ccaccggcac aaaccagatc tcttcagctg
     3361 ggacagagtg gtcaaaatgt ccccaactga gagacttgaa catgctttta gcaaggccca
     3421 tacttatttg gggattgaaa aacttctgga tcctgaagat gttgccgtgc agctccctga
     3481 caagaaatcc ataattatgt atttaacgtc tctgtttgag gtgcttcctc agcaagtcac
     3541 tatagaccaa ttgaagctct caggtggtgg agggagcggc ggaggtggct ctggaggtgg
     3601 tggctcagct gaagcatggt acaatctggg aaacgcttat tacaagcagg gcgactatca
     3661 aaaagccatt gagtattacc aaaaggctct tgagctagac cctaataacg ccgaagcgtg
     3721 gtataaccta ggcaacgcat attacaagca gggagactac caaaaggcaa tcgaatatta
     3781 ccagaaggca ctcgaactgg acccaaataa cgcagaggct tggtataatc tgggaaacgc
     3841 ctactataaa cagggtgact accaaaaggc gatagaggac tatcagaagg ccttggagct
     3901 cgatccgaac aatctgcaac gtagtgctgg tggcggaggg tctggtggag gaggctcagg
     3961 aggaggtggc gcatcaaacg aattccagtg ccggatctgc atgcggaact tcagcgaccg
     4021 gtccaacctg agcaggcaca tcagaaccca caccggagaa aagcccttcg cctgcgacat
     4081 ttgcggccgg aagttcgcca tcagcagcaa cctgaacagc cacaccaaga tccacactgg
     4141 cagccagaaa cctttccagt gcagaatttg tatgagaaac tttagcagaa gcgacaacct
     4201 ggccagacac atccggacac atactggtga aaaacctttt gcctgtgata tctgtggcag
     4261 aaagtttgcc acctccggca atctgacccg gcacacaaag attcacctgc ggggcagcca
     4321 gctatcgatt ggctccggag ccacgaactt ctctctgtta aagcaagcag gagacgtgga
     4381 agaaaacccc ggtcccatgg taagtatcgc tctgcaggct ggttacgacc tactgggtga
     4441 cggcagacct gaaaccctgt ggctgggcat cggcactctg ctgatgctga ttggaacctt
     4501 ctactttctg gtccgcggat ggggagtcac cgataaggat gcccgggaat attacgctgt
     4561 gactatcctg gtgtccggaa tcgcatccgc cgcatatctg tctatgttct ttggtatcgg
     4621 gcttactgag gtgtccgtcg ggggcgaaat gttggatatc tattatgcca ggtacgccca
     4681 gtggctgttt accaccccac ttctgctgct gcacctggcc cttctcgcta aggtggatcg
     4741 ggtgaccatc ggcaccctgg tgggtgtgga cgccctgatg atcgtcactg gcctcatcgg
     4801 agcattgagc cacacggcca tagccagata cagttggtgg ttgttctcta caatttgcat
     4861 gatagtggtg ctctatgttc tggctacatc cctgcgatct gctgcaaggg agcggggccc
     4921 cgaggtggca tctaccttta acaccctgac agctctggtc ttggtgctgt ggaccgctta
     4981 ccctatcctg tggatcatag gcactgaggg cgctggcgtg gtgggcctgg gcatcgaaac
     5041 tctgctgttt atggtgttgg acgtgactgc caaggtcggc tttggcttta tcctgttgag
     5101 atcccgggct attctgggcg acaccgaggc accagaaccc agtgccggtg ccgatacgtt
     5161 gatgaagagt agaatcacaa gcgaaggcga gtacatcccc ctggatcaaa tagacataaa
     5221 tgtagtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5281 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5341 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5401 cctcgtgacc accttcggcg gcggcctgat gtgcttcgcc cgctaccccg accacatgaa
     5461 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5521 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5581 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5641 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     5701 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     5761 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5821 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5881 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaatc
     5941 aaggatcacc tctgagggcg agtatatccc tctggatcag atcgacatta acgtcggtgg
     6001 aaaatcccgc ataacctctg agggcgagta catccctctg gatcaaatcg atataaacgt
     6061 ggggggaaaa agtcgaatca cttctgaggg cgagtatata cccctcgatc agattgacat
     6121 caatgtgggg ggcttttgtt atgagaatga agtgtaggcg gccgcactcc tcaggtgcag
     6181 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     6241 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     6301 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     6361 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     6421 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     6481 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     6541 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     6601 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     6661 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
     6721 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6781 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6841 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
     6901 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6961 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7021 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7081 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7201 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
     7261 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7321 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7381 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7441 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7501 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7561 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7621 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7681 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7741 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7801 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7861 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7921 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7981 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8041 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8101 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8161 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8221 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8281 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8341 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8401 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8461 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8521 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8581 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8641 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8701 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8761 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8821 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8881 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8941 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9001 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9061 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9121 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9181 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtc
//
