LOCUS       sequence-205263-       11759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synOptopatch construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE
  TITLE     All-optical synaptic electrophysiology probes mechanism of 
            ketamine-induced disinhibition.
  JOURNAL   Nat Methods. 2018 Oct;15(10):823-831. doi: 
            10.1038/s41592-018-0142-8. Epub 2018 Oct 1.
  PUBMED    30275587
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0142-8"; journalName: "Nat Methods"; date: 
            "2018-10"; volume: "15"; issue: "10"; pages: "823-831"
COMMENT     Sequence Label: LZF80: Cre-on CAG-somCheRiff-CFP (DIO)
FEATURES             Location/Qualifiers
     source          1..11759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    264..389
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    882..1115
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1300..1344
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1642..1759
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1875..2178
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2180..2453
                     /label=chicken beta-actin promoter
     primer_bind     3262..3284
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3346..3365
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3427..3460
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3523..3556
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(3768..4478,4479..4481))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3810..3831)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4159..4178
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4418..4439
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4497..5444)
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="MGGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQ
                     CSSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKV
                     ILEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALL
                     VSDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLV
                     LAMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKI
                     HEHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     regulatory      5441..5450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5463..5496)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(5543..5576)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5617..6205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5670..5690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6088..6099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6208..6224)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6209..6225)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6730..6910
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6936..6953)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6942..7166
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7212..7640
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7299..7318)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7509..7530
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7649..7669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7654..7983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7834..7969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7896..7915
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8031..8078
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8097..8471
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8601..8722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8638..8657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8692..8711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8771..8787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8771..8787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8784..8806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8795..8811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8819..8825,8826..8843,8844..8849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8864..8885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9002..9019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9173..9761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9253..9272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9932..10723,10724..10792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10555..10574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10793..10897)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10972..10991)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11163..11542
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11544..11742
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11697..11717
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtacgtnta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
      241 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      301 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      361 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      421 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      481 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      541 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      601 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      661 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      721 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      781 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
      841 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
      901 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
      961 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1021 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1081 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1141 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1201 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1261 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1321 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1381 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1441 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1501 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1561 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1621 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1681 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1741 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1801 taactgcagg aatctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1861 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1921 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1981 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2041 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2101 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2161 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     2221 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     2281 ggggcggggg gggggggcgc gcgccaggcg gggcggggcg gggsgvgggg cggggcgggg
     2341 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     2401 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     2461 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     2521 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     2581 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     2641 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     2701 tgtgcgtggg gagcgccgcg tgcggctccg cgcgagggga gcgcggccgg gggcggtgcc
     2761 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     2821 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     2881 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     2941 agggctcggg ggaggggcgc ggcggtcccc ggagcgccgg cggctgtcga ggcgcggcga
     3001 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     3061 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     3121 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     3181 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     3241 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     3301 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     3361 ttgtgctgtc tcatcatttt ggcaaagaat tgatttgata ccgcgggccc gggatctcga
     3421 ggatccataa cttcgtataa agtatcctat acgaagttat atcaaaatag gaagaccaat
     3481 gcttcaccat cgacccgaat tgccaagcat caccatcgac ccataacttc gtataatgta
     3541 tgctatacga agttatacta gctagctaat tagaatctgg tggcttcagg gaagtccgtg
     3601 gcacagctga tgaagctgtc aatgctggac atgctccgca tgtcgatgac gccctccgtg
     3661 cgtgcgggca gaggggccac ggggctgggc atgctgctca tctccagctc ctctggaggc
     3721 ttgctctgcg gggccatctc cttggtgttg aggatgggct gggactgctt gtacagctcg
     3781 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     3841 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
     3901 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     3961 ccgtcctcga tgttgtggcg gatcttgaag tgggccttga tgccgttctt ctgcttgtcg
     4021 gcggtgatat agacgttgtg gctgatgtag ttgtactcca gcttgtgccc caggatgttg
     4081 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     4141 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtacgctcc
     4201 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     4261 tagcggctga agcactgcac gccccaggtc agggtggtca cgagggtggg ccagggcacg
     4321 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     4381 tcgccctcgc cggacacgct gaacctgtgg ccgtttacgt cgccgtccag ctcgaccagg
     4441 atgggcacca ccccggtgaa cagctcctcg cccttgctca cgctcttggc ggccgccact
     4501 ttatcttcct ctgtcacgaa ggccagcacg tccaccttgc gtcccagaaa ctgggagctc
     4561 actggtctcc tgatatcgcc gtacataatg atatgctcgt gaattttgat ccgcaggaag
     4621 tgccccagca gtccccaaat attcttggac agcaggtcgg cgatggtatg gccaatagtg
     4681 gacccagcca cgctcagggt gtgcatgcct tcaggcccaa agatgaacag gccgggaaac
     4741 atcagccaac tagaatagta cacggcagtc atggccagca ccagtttctt acagccgccg
     4801 gcaggcatga tatagtaaga ctccacgtag atgattccag cgttgtagaa tgtctgggtt
     4861 ccatacacca ggccgataca gtaaaacagc cacttcaccc acccagtggc cagagcggct
     4921 gtcactccca tgcaaatagt tcccaggtcg gacaccagca gagccattgt ccgcttattg
     4981 tatgcctcac tcaggccggt gatgttagac agatgaatca ggatcacggg acatgtcagc
     5041 agccattcgg catatctcag ccatggggta atgttccctc cgtacaggaa cagcatagca
     5101 ggactggtga actcgaaata gatctccaga atcactttga tcagctccac ggagcacacg
     5161 tacacttcct cccatcccac gctggcctta taggcgtgcc agccgtagaa agccagaaac
     5221 agggcagaca ggaacacggc aaaccactgg aagcccctag ctgtttcctg ctcccacagg
     5281 gccccatagt agatgtagat ggagctgcac tgttcctcat acccatgcac ggggtggtag
     5341 ggtggattca cttgatatcc agctggggca tggtactcac tgcctccggc agaatcgttt
     5401 ccaggtgggg cgctgtgagc gtctggagca ggagctccgc ccatggtggc ggcgcgccta
     5461 gtataacttc gtataggata ctttatacga agttatcatt gggattcttc ctattttgat
     5521 ccaagcatca ccatcgaccc ccataacttc gtatagcata cattatacga agttattccc
     5581 tcgaagaggt tcgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     5641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6001 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6181 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
     6241 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     6301 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     6361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6421 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     6481 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     6541 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     6601 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     6661 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     6721 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6901 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     6961 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     7021 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     7081 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     7141 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     7201 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7261 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7321 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7381 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7441 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7501 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7561 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7621 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7921 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7981 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     8041 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     8101 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     8161 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     8221 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     8281 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     8341 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     8401 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     8461 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     8521 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8581 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     8641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8701 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     8761 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8821 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8881 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8941 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9001 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9061 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9781 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9841 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9901 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9961 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10021 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10081 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10141 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10201 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10261 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10321 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10381 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10441 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10501 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10561 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10621 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10681 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10741 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10801 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10861 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10921 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
    10981 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
    11041 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
    11101 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
    11161 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    11221 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    11281 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    11341 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    11401 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
    11461 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
    11521 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
    11581 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
    11641 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
    11701 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtact
//
