LOCUS       sequence-205265-       10806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synOptopatch construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE
  TITLE     All-optical synaptic electrophysiology probes mechanism of 
            ketamine-induced disinhibition.
  JOURNAL   Nat Methods. 2018 Oct;15(10):823-831. doi: 
            10.1038/s41592-018-0142-8. Epub 2018 Oct 1.
  PUBMED    30275587
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0142-8"; journalName: "Nat Methods"; date: 
            "2018-10"; volume: "15"; issue: "10"; pages: "823-831"
COMMENT     Sequence Label: LZF43: Cre-on CAG-CheRiff-CFP (DIO)
FEATURES             Location/Qualifiers
     source          1..10806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2436..2739
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3041..3063
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3125..3144
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3206..3239
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3302..3335
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(3349..4062,4063..4065))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3394..3415)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3743..3762
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4002..4023
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4135..5082)
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="MGGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQ
                     CSSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKV
                     ILEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALL
                     VSDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLV
                     LAMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKI
                     HEHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     regulatory      5079..5088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5107..5140)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(5187..5220)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5261..5849
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5314..5334)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5732..5743)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5852..5868)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5853..5869)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6374..6554
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6580..6597)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6586..6810
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6856..7284
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6943..6962)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7153..7174
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7293..7313)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7298..7627
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7478..7613
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7540..7559
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7675..7722
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7741..8115
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8245..8366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8282..8301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8336..8355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8415..8431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8415..8431)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8428..8450)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8439..8455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8463..8469,8470..8487,8488..8493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8508..8529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8646..8663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8817..9405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8897..8916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9576..10367,10368..10436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10199..10218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10437..10541)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10616..10635)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaactgcag gaatctagtt attaatagta atcaattacg
     2401 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2461 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2521 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2581 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2641 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2701 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     2761 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     2821 tagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     2881 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     2941 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     3001 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     3061 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     3121 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt gatttgatac
     3181 cgcgggcccg ggatctcgag gatccataac ttcgtataaa gtatcctata cgaagttata
     3241 tcaaaatagg aagaccaatg cttcaccatc gacccgaatt gccaagcatc accatcgacc
     3301 cataacttcg tataatgtat gctatacgaa gttatactag ctagctaatt acttgtacag
     3361 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     3421 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     3481 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     3541 gctgccgtcc tcgatgttgt ggcggatctt gaagtgggcc ttgatgccgt tcttctgctt
     3601 gtcggcggtg atatagacgt tgtggctgat gtagttgtac tccagcttgt gccccaggat
     3661 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     3721 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtacg
     3781 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     3841 ggggtagcgg ctgaagcact gcacgcccca ggtcagggtg gtcacgaggg tgggccaggg
     3901 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     3961 gccctcgccc tcgccggaca cgctgaacct gtggccgttt acgtcgccgt ccagctcgac
     4021 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccacgt tgatgtcgat
     4081 ctggtccagg gggatgtact cgccctcgct ggtgatcctg ctcttggcgg ccgccacttt
     4141 atcttcctct gtcacgaagg ccagcacgtc caccttgcgt cccagaaact gggagctcac
     4201 tggtctcctg atatcgccgt acataatgat atgctcgtga attttgatcc gcaggaagtg
     4261 ccccagcagt ccccaaatat tcttggacag caggtcggcg atggtatggc caatagtgga
     4321 cccagccacg ctcagggtgt gcatgccttc aggcccaaag atgaacaggc cgggaaacat
     4381 cagccaacta gaatagtaca cggcagtcat ggccagcacc agtttcttac agccgccggc
     4441 aggcatgata tagtaagact ccacgtagat gattccagcg ttgtagaatg tctgggttcc
     4501 atacaccagg ccgatacagt aaaacagcca cttcacccac ccagtggcca gagcggctgt
     4561 cactcccatg caaatagttc ccaggtcgga caccagcaga gccattgtcc gcttattgta
     4621 tgcctcactc aggccggtga tgttagacag atgaatcagg atcacgggac atgtcagcag
     4681 ccattcggca tatctcagcc atggggtaat gttccctccg tacaggaaca gcatagcagg
     4741 actggtgaac tcgaaataga tctccagaat cactttgatc agctccacgg agcacacgta
     4801 cacttcctcc catcccacgc tggccttata ggcgtgccag ccgtagaaag ccagaaacag
     4861 ggcagacagg aacacggcaa accactggaa gcccctagct gtttcctgct cccacagggc
     4921 cccatagtag atgtagatgg agctgcactg ttcctcatac ccatgcacgg ggtggtaggg
     4981 tggattcact tgatatccag ctggggcatg gtactcactg cctccggcag aatcgtttcc
     5041 aggtggggcg ctgtgagcgt ctggagcagg agctccgccc atggtggcgg cctaggcgcg
     5101 cctagtataa cttcgtatag gatactttat acgaagttat cattgggatt cttcctattt
     5161 tgatccaagc atcaccatcg acccccataa cttcgtatag catacattat acgaagttat
     5221 tccctcgaag aggttcgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     5281 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5341 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5401 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5461 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5521 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5581 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5641 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     5701 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5761 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5821 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     5881 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     5941 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     6001 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6061 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     6121 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     6181 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     6241 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     6301 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     6361 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6421 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6481 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6541 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6601 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6661 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6721 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6781 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6841 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6901 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6961 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7021 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7081 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7141 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7201 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7261 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7321 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7381 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7441 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7501 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7561 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7621 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     7681 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     7741 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     7801 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     7861 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     7921 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     7981 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     8041 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     8101 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8161 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8221 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     8281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8341 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8401 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8461 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8521 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8581 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8641 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8701 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8761 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8821 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8881 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8941 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9001 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9061 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9121 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9181 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9241 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9301 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9361 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9421 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9481 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9541 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9601 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9661 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9721 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9781 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9841 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9901 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9961 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10021 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10081 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10141 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10201 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10261 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10321 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10381 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10441 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10501 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10561 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
    10621 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
    10681 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
    10741 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
    10801 cgcgtt
//
