LOCUS       sequence-205268-       10251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synOptopatch construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE
  TITLE     All-optical synaptic electrophysiology probes mechanism of 
            ketamine-induced disinhibition.
  JOURNAL   Nat Methods. 2018 Oct;15(10):823-831. doi: 
            10.1038/s41592-018-0142-8. Epub 2018 Oct 1.
  PUBMED    30275587
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0142-8"; journalName: "Nat Methods"; date: 
            "2018-10"; volume: "15"; issue: "10"; pages: "823-831"
COMMENT     Sequence Label: LZF39: Cre-off hSyn-CheRiff-CFP (FAS)
FEATURES             Location/Qualifiers
     source          1..10251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2395..2842
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2895..2904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2901..3848
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="MGGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQ
                     CSSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKV
                     ILEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALL
                     VSDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLV
                     LAMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKI
                     HEHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             join(3918..3920,3921..4634)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3960..3981)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4221..4240)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4568..4589
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4706..5294
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4759..4779)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5177..5188)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5297..5313)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5298..5314)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5819..5999
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6025..6042)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6031..6255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6301..6729
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6388..6407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6598..6619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6738..6758)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6743..7072
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6923..7058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6985..7004
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7120..7167
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7186..7560
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7690..7811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7727..7746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7781..7800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7860..7876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7860..7876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7873..7895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7884..7900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7908..7914,7915..7932,7933..7938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7953..7974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8091..8108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8262..8850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8342..8361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9021..9812,9813..9881))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9644..9663
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9882..9986)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10061..10080)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaagtgtct agactgcaga gggccctgcg tatgagtgca
     2401 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
     2461 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
     2521 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
     2581 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
     2641 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
     2701 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
     2761 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
     2821 cgtgtcgtgc ctgagagcgc agtcgagaag gatccacaac ttcgtatata cctttctata
     2881 cgaagttgtg gcgcgccacc atgggcggag ctcctgctcc agacgctcac agcgccccac
     2941 ctggaaacga ttctgccgga ggcagtgagt accatgcccc agctggatat caagtgaatc
     3001 caccctacca ccccgtgcat gggtatgagg aacagtgcag ctccatctac atctactatg
     3061 gggccctgtg ggagcaggaa acagctaggg gcttccagtg gtttgccgtg ttcctgtctg
     3121 ccctgtttct ggctttctac ggctggcacg cctataaggc cagcgtggga tgggaggaag
     3181 tgtacgtgtg ctccgtggag ctgatcaaag tgattctgga gatctatttc gagttcacca
     3241 gtcctgctat gctgttcctg tacggaggga acattacccc atggctgaga tatgccgaat
     3301 ggctgctgac atgtcccgtg atcctgattc atctgtctaa catcaccggc ctgagtgagg
     3361 catacaataa gcggacaatg gctctgctgg tgtccgacct gggaactatt tgcatgggag
     3421 tgacagccgc tctggccact gggtgggtga agtggctgtt ttactgtatc ggcctggtgt
     3481 atggaaccca gacattctac aacgctggaa tcatctacgt ggagtcttac tatatcatgc
     3541 ctgccggcgg ctgtaagaaa ctggtgctgg ccatgactgc cgtgtactat tctagttggc
     3601 tgatgtttcc cggcctgttc atctttgggc ctgaaggcat gcacaccctg agcgtggctg
     3661 ggtccactat tggccatacc atcgccgacc tgctgtccaa gaatatttgg ggactgctgg
     3721 ggcacttcct gcggatcaaa attcacgagc atatcattat gtacggcgat atcaggagac
     3781 cagtgagctc ccagtttctg ggacgcaagg tggacgtgct ggccttcgtg acagaggaag
     3841 ataaagtggc ggccgccaag agcaggatca ccagcgaggg cgagtacatc cccctggacc
     3901 agatcgacat caacgtggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3961 tggtcgagct ggacggcgac gtaaacggcc acaggttcag cgtgtccggc gagggcgagg
     4021 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4081 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc
     4141 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4201 agcgtaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4261 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4321 acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg
     4381 acaagcagaa gaacggcatc aaggcccact tcaagatccg ccacaacatc gaggacggca
     4441 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4501 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     4561 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4621 agctgtacaa gtaattagct agctaaacaa cttcgtatat acctttctat acgaagttgt
     4681 cgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     4741 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4801 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4861 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4921 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4981 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5041 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5101 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5161 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5221 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5281 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     5341 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     5401 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     5461 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5521 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     5581 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     5641 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     5701 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     5761 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     5821 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5881 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5941 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6001 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6061 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6121 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6181 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6241 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6301 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6361 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6421 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6481 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6601 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6661 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6721 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6781 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6841 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6901 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6961 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7021 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7081 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     7141 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     7201 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     7261 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     7321 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     7381 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     7441 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     7501 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     7561 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7621 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7681 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7741 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7801 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     7861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8701 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    10021 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
    10081 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    10141 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    10201 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt t
//
