LOCUS       sequence-205269-        9177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synOptopatch construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE
  TITLE     All-optical synaptic electrophysiology probes mechanism of 
            ketamine-induced disinhibition.
  JOURNAL   Nat Methods. 2018 Oct;15(10):823-831. doi: 
            10.1038/s41592-018-0142-8. Epub 2018 Oct 1.
  PUBMED    30275587
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0142-8"; journalName: "Nat Methods"; date: 
            "2018-10"; volume: "15"; issue: "10"; pages: "823-831"
COMMENT     Sequence Label: LZF1723: mI12b-EGFP
FEATURES             Location/Qualifiers
     source          1..9177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2866..2875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2872..2874,2875..2877,2878..3591)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2872..2874,2875..2877,2878..3588)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2917..2938)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3178..3197)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3525..3546
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3632..4220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3685..3705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4103..4114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4223..4239)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4224..4240)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4745..4925
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4951..4968)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4957..5181
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5227..5655
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5314..5333)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5524..5545
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5664..5684)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5669..5998
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5849..5984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5911..5930
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6046..6093
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6112..6486
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6616..6737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6653..6672)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6707..6726
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6786..6802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6786..6802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6799..6821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6810..6826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6834..6840,6841..6858,6859..6864))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6879..6900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7017..7034)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7188..7776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7268..7287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7947..8738,8739..8807))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8570..8589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8808..8912)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8987..9006)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaagcgtac agctgcaaac ccaagagggt cagcatcatt
     2401 tcactgtatt ctcttcttga ttacaagccg ggcccatcaa acacaacata attacagtaa
     2461 tttcaggttt atttattcta atgcagtttc cccatctctc tggtaattat gagcaatttt
     2521 ttcgcccagg gaatcttttt gcattaacaa aagagataac gcactgaaag ccaaatttgc
     2581 tgcgcattga gaaaagggaa aaaaaaatca aataggtgcg agctgccatc tctgcaattc
     2641 tccggtaccg gagcccgcaa attgcttgca ggtgtatgga gcaagcttgt caatggccgg
     2701 gcctccaaat tagcaaatgc acagcagcga agtaatgaag acagacttag caaaattgct
     2761 aaacaacaga tacctcttta atatcctctc tctcacacac gagctctaaa gggggtaggg
     2821 gtgggggtgg ggcacagtcc caacctttcc ctcactggac cggtcgccac catggtgagc
     2881 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2941 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     3001 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     3061 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     3121 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     3181 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     3241 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3301 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     3361 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3421 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3481 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3541 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc
     3601 gactctagaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     3661 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3721 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3781 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3841 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3901 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3961 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4021 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4081 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4141 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4201 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     4261 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     4321 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     4381 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4441 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     4501 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     4561 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     4621 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     4681 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     4741 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4801 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4861 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4921 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     4981 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5041 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5101 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5161 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     5221 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5281 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5341 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5401 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5461 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5521 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5581 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5641 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5821 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5881 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     5941 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6001 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     6061 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     6121 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     6181 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     6241 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     6301 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     6361 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     6421 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     6481 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6541 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6601 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6721 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7021 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7081 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7141 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7201 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7261 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7321 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7381 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7501 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7561 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7621 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7681 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7741 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7801 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7861 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8881 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8941 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     9001 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9061 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9121 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
