LOCUS       sequence-205282-        3196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3196)
  TITLE     Moravec lab plasmids
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDB1alpha13R:empty
FEATURES             Location/Qualifiers
     source          1..3196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      189..624
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    751..773
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     primer_bind     894..913
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1107..1129
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1121..1138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1122..1138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1139..1195
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1208..1224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1208..1224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1221..1243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1232..1248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1256..1262,1263..1280,1281..1286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1301..1322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1405..1429
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1520..2108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1600..1619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2279..3094)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2279..3094)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3002..3021
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3086..3105
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc
       61 cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta
      121 caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca
      181 ggcgatcccc atccaacagc ccgccgtcga gcgggctttt ttatccccgg aagcctgtgg
      241 atagagggta gttatccacg tgaaaccgct aatgccccgc aaagccttga ttcacggggc
      301 tttccggccc gctccaaaaa ctatccacgt gaaatcgcta atcagggtac gtgaaatcgc
      361 taatcggagt acgtgaaatc gctaataagg tcacgtgaaa tcgctaatca aaaaggcacg
      421 tgagaacgct aatagccctt tcagatcaac agcttgcaaa cacccctcgc tccggcaagt
      481 agttacagca agtagtatgt tcaattagct tttcaattat gaatatatat atcaattatt
      541 ggtcgccctt ggcttgtgga caatgcgcta cgcgcaccgg ctccgcccgt ggacaaccgc
      601 aagcggttgc ccaccgtcga gcgcctttgc ccacaacccg gcggccggcc gcaacagatc
      661 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct
      721 tctggatttc cgatccccgg aattagatct tggcaggata tattgtggtg taaagaattc
      781 gtctcagctt agcgagagac ccacagcttg tctgtaagcg gatgccggga gcagacaagc
      841 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
      901 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
      961 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     1021 atcggcgcag gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     1081 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     1141 attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt
     1201 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1261 tacgagccgg aagcatatag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1321 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctggggt
     1381 ctctctccgg tatgagacga attctgacag gatatattgg cgggtaaact aagtcgctgt
     1441 atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1501 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1561 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1621 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1681 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1741 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1801 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1861 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1921 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1981 ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa
     2041 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2101 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2161 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2221 attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg tctagtgatt
     2281 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     2341 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     2401 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     2461 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     2521 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     2581 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     2641 cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     2701 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     2761 cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac catgcatcat
     2821 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     2881 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     2941 actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat tgcccgacat
     3001 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     3061 ttgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     3121 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     3181 gattttgaga cacaac
//
