LOCUS       sequence-205284-        3198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3198)
  TITLE     Moravec lab plasmids
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDB1alpha13R:empty
FEATURES             Location/Qualifiers
     source          1..3198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     75..94
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      374..809
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    938..960
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     primer_bind     1081..1100
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1294..1316
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1308..1325
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1309..1325
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1326..1382
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1395..1411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1395..1411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1408..1430)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1419..1435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1443..1449,1450..1467,1468..1473))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1488..1509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1592..1616
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1707..2295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1787..1806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2466..3198,1..83))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     join(3189..3198,1..10)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac
       61 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
      121 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
      181 acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat
      241 cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc
      301 acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg
      361 attcaggcga tccccatcca acagcccgcc gtcgagcggg cttttttatc cccggaagcc
      421 tgtggataga gggtagttat ccacgtgaaa ccgctaatgc cccgcaaagc cttgattcac
      481 ggggctttcc ggcccgctcc aaaaactatc cacgtgaaat cgctaatcag ggtacgtgaa
      541 atcgctaatc ggagtacgtg aaatcgctaa taaggtcacg tgaaatcgct aatcaaaaag
      601 gcacgtgaga acgctaatag ccctttcaga tcaacagctt gcaaacaccc ctcgctccgg
      661 caagtagtta cagcaagtag tatgttcaat tagcttttca attatgaata tatatatcaa
      721 ttattggtcg cccttggctt gtggacaatg cgctacgcgc accggctccg cccgtggaca
      781 accgcaagcg gttgcccacc gtcgagcgcc tttgcccaca acccggcggc cggccgcaac
      841 agatcgtttt ataaattttt ttttttgaaa aagaaaaagc ccgaaaggcg gcaacctctc
      901 gggcttctgg atttccgatc cccggaatta gagatcttgg caggatatat tgtggtgtaa
      961 agaattcgtc tcagcttagc gagagaccca cagcttgtct gtaagcggat gccgggagca
     1021 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     1081 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     1141 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     1201 aagggcgatc ggcgcaggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     1261 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1321 ccagtgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc
     1381 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     1441 cacaacatac gagccggaag catatagtgt aaagcctggg gtgcctaatg agtgagctaa
     1501 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1561 ctggggtctc tctccggtat gagacgaatt ctgacaggat atattggcgg gtaaactaag
     1621 tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa ggccaggaac
     1681 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1741 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1801 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1861 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1921 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1981 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2041 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2101 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2161 atctgcgctc tgctgaagcc agttaccttc ggaagaagag ttggtagctc ttgatccggc
     2221 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2281 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2341 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2401 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgtgta acattggtct
     2461 agtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     2521 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     2581 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     2641 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     2701 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     2761 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     2821 gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     2881 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     2941 ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat
     3001 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     3061 cagtttagtc tgaccatctc atctgtaaca acattggcaa cgctaccttt gccatgtttc
     3121 agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc acctgattgc
     3181 ccgacattat cgcgagcc
//
