LOCUS       sequence-205286-        3192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3192)
  TITLE     Moravec lab plasmids
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDB1alpha11:empty
FEATURES             Location/Qualifiers
     source          1..3192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      213..648
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    775..797
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     primer_bind     914..933
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1127..1149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1141..1158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1142..1158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1159..1215
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1228..1244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1228..1244)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1241..1263)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1252..1268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1276..1282,1283..1300,1301..1306))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1321..1342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1425..1449
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1540..2128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1620..1639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2299..3114)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2299..3114)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3022..3041
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3106..3125
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt
       61 gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt
      121 caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt
      181 ctggctggat gatggggcga ttcaggcgat ccccatccaa cagcccgccg tcgagcgggc
      241 ttttttatcc ccggaagcct gtggatagag ggtagttatc cacgtgaaac cgctaatgcc
      301 ccgcaaagcc ttgattcacg gggctttccg gcccgctcca aaaactatcc acgtgaaatc
      361 gctaatcagg gtacgtgaaa tcgctaatcg gagtacgtga aatcgctaat aaggtcacgt
      421 gaaatcgcta atcaaaaagg cacgtgagaa cgctaatagc cctttcagat caacagcttg
      481 caaacacccc tcgctccggc aagtagttac agcaagtagt atgttcaatt agcttttcaa
      541 ttatgaatat atatatcaat tattggtcgc ccttggcttg tggacaatgc gctacgcgca
      601 ccggctccgc ccgtggacaa ccgcaagcgg ttgcccaccg tcgagcgcct ttgcccacaa
      661 cccggcggcc ggccgcaaca gatcgtttta taaatttttt tttttgaaaa agaaaaagcc
      721 cgaaaggcgg caacctctcg ggcttctgga tttccgatcc ccggaattag atcttggcag
      781 gatatattgt ggtgtaaaga attcgtctca ggagagagac ccacagcttg tctgtaagcg
      841 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      901 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
      961 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     1021 cgcaactgtt gggaagggcg atcggcgcag gcctcttcgc tattacgcca gctggcgaaa
     1081 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     1141 tgtaaaacga cggccagtga attcgagctc ggtacccggg gatcctctag agtcgacctg
     1201 caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1261 gctcacaatt ccacacaaca tacgagccgg aagcatatag tgtaaagcct ggggtgccta
     1321 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1381 cctgtcgtgc cagctggggt ctctcgctcc attgagacga attctgacag gatatattgg
     1441 cgggtaaact aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa aaggccagca
     1501 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1561 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1621 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1681 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1741 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1801 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1861 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1921 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1981 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa gagttggtag
     2041 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2101 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2161 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2221 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatgt
     2281 gtaacattgg tctagtgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     2341 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     2401 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     2461 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     2521 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     2581 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     2641 gttattcatt cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca
     2701 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     2761 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt
     2821 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     2881 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     2941 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc ggtagattgt
     3001 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     3061 gttggaattt aatcgcggcc ttgagcaaga cgtttcccgt tgaatatggc tcataacacc
     3121 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc
     3181 ttgtgcaatg ta
//
