LOCUS       sequence-205291-        1857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PKAcs-GFP fusion in mammalian cells with flexible linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Norris JL, Hughes RM
  TITLE     protaTETHER - a method for the incorporation of variable linkers in 
            protein fusions reveals impacts of linker flexibility in a PKAc-GFP 
            fusion protein.
  JOURNAL   FEBS Open Bio. 2018 Apr 25;8(6):1029-1042. doi: 
            10.1002/2211-5463.12414. eCollection 2018 Jun.
  PUBMED    29928581
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/2211-5463.12414"; journalName: "FEBS Open Bio"; date: 
            "2018-04-25- 25"; volume: "8"; issue: "6"; pages: "1029-1042"
COMMENT     Sequence Label: PKAcs-G12X-GFP
FEATURES             Location/Qualifiers
     source          1..1857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1123..1151)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1746..1766
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgggcaacg ccgccgccgc caagaagggc agcgagcagg agagcgtgaa agagttccta
       61 gccaaagcca aggaagattt cctgaaaaaa tgggagaccc cttctcagaa tacagcccag
      121 ttggatcagt ttgatagaat caagaccctt ggcaccggct cctttgggcg agtgatgctg
      181 gtgaagcaca aggagagtgg gaaccactac gccatgaaga tcttagacaa gcagaaggtg
      241 gtgaagctaa agcagatcga gcagactctg aatgagaagc gcatcctgca ggccgtcaac
      301 ttcccgttcc tggtcaaact tgaattctcc ttcaaggaca actcaaacct gtacatggtc
      361 atggagtatg tagctggtgg cgagatgttc tcccacctac ggcggattgg aaggttcagc
      421 gagccccatg cccgtttcta cgcggcgcag atcgtcctga cctttgagta tctgcactcc
      481 ctggacctca tctaccggga cctgaagccc gagaatcttc tcatcgacca gcagggctat
      541 attcaggtga cagacttcgg ttttgccaag cgtgtgaaag gccgtactcg gaccttgtgt
      601 gggacccctg agtacttggc ccccgagatt atcctgagca aaggctacaa caaggctgtg
      661 gactggtggg ctctcggagt cctcatctac gagatggctg ctggttaccc acccttcttc
      721 gctgaccagc ctatccagat ctatgagaaa atcgtctctg ggaaggtgcg gttcccatcc
      781 cacttcagct ctgacttgaa ggacctgctg cggaaccttc tgcaagtgga tctcaccaag
      841 cgctttggga acctcaagaa cggggtcaat gacatcaaga accacaagtg gtttgccacg
      901 actgactgga ttgccatcta tcagagaaag gtggaagctc ccttcatacc aaagtttaaa
      961 ggccctgggg acacgagtaa ctttgacgac tatgaggagg aagagatccg ggtctccatc
     1021 aatgagaagt gtggcaagga gtttactgag tttggcggcg gcggcggcgg cggcggcggc
     1081 ggcggcggca tggctagcaa aggagaagaa ctcttcactg gagttgtccc aattcttgtt
     1141 gaattagatg gtgatgttaa cggccacaag ttctctgtca gtggagaggg tgaaggtgat
     1201 gcaacatacg gaaaacttac cctgaagttc atctgcacta ctggcaaact gcctgttcca
     1261 tggccaacac tagtcactac tctgtgctat ggtgttcaat gcttttcaag atacccggat
     1321 catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaagg
     1381 accatcttct tcaaagatga cggcaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1441 gatacccttg ttaatagaat cgagttaaaa ggtattgact tcaaggaaga tggcaacatt
     1501 ctgggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1561 caaaagaatg gaatcaaagt gaacttcaag acccgccaca acattgaaga tggaagcgtt
     1621 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1681 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1741 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcag gatgaactgt
     1801 acaactgaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg gtacaag
//
