LOCUS       sequence-205294-        9840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cloning of a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9840)
  TITLE     David Root CRISPR plasmids
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pXPR_045
FEATURES             Location/Qualifiers
     source          1..9840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    100..121
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(136..141,142..159,160..166)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    174..190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     179..201
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     198..214
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     198..214
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     233..253
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        235..253
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        281..507
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             508..688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    735..860
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1353..1586
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1771..1815
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2332..2580
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2332..2352
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2503..2522
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4462..4537
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4593..4710
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4817..5327
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5246..5264
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5342..5830
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    5846..6434
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5899..5919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6317..6328)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6437..6453)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6438..6454)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6639..6872
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6944..7065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6981..7000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7035..7054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7105..7240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7260..7279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7261..7279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7289..7306)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7289..7305)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7298..7320)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7447..7902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7534..7553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7744..7765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7928..8032
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8033..8101,8102..8893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8251..8270)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9064..9652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9553..9572
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9806..9823
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
       61 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      121 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      181 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
      241 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
      301 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
      361 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
      421 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
      481 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
      541 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      601 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      661 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
      721 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
      781 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
      841 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
      901 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
      961 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1021 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1081 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1141 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1201 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1261 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1321 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1381 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1441 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1501 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1561 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     1621 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     1681 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     1741 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     1801 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     1861 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     1921 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     1981 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     2041 tccattcgat tagtgaacgg atctcgacgg tatcgatcac gagactagcc tcgaccacga
     2101 cgcaatggag aaagacggag agcgccaacg gcgtccatct cgaaggagtc gccagcgata
     2161 accggagtag ttgaaatggt aataagacga ccaatctgac cagcaaggaa gccaagatgg
     2221 gaaaggtcat gcggcatacg ctcggcgcca gtttgaatat tagacataat ttatcctcaa
     2281 gtaagggggt acacgcgtca gtcgagtcct gtcgacaagg tcgggcagga agagggccta
     2341 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga
     2401 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     2461 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     2521 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     2581 ggagacggtt gtaaatgagc acacaaaata cacatgctaa aatattatat tctatgacct
     2641 ttataaaatc aaccaaaatc ttctttttaa taactttagt atcaataatt agaattttta
     2701 tgttcctttt tgcaaacttt taataaaaat gagcaaaata aaaaaacgct agttttagta
     2761 actcgcgttg ttttcttcac ctttaataat agctactcca ccacttgttc ctaagcggtc
     2821 agctcctgct tcaatcattt tttgagcatc ttcaaatgtt ctaactccac cagctgcttt
     2881 aactaaagca ttgtctttaa caactgactt cattagttta acatcttcaa atgttgcacc
     2941 tgattttgaa aatcctgttg atgttttaac aaattctaat ccagcttcaa cagctatttc
     3001 acaagctttc atgatttctt cttttgttaa taaacaattt tccataatac atttaacaac
     3061 atgtgatcca gctgcttttt ttacagcttt catgtcttct aaaactaatt cataattttt
     3121 gtcttttaat gcaccaatat ttaataccat atcaatttct gttgcaccat ctttaattgc
     3181 ttcagaaact tcgaatgctt ttgtagctgt tgtgcatgca cctagaggaa aacctacaac
     3241 atttgttatt cctacatttg tgccttttaa taattcttta caatagcttg ttcaatatga
     3301 attaacacaa actgttgcaa aatcaaattc aattgcttca tcacataatt gtttaatttc
     3361 agctttcgta gcatcttgtt ttaataatgt gtgatctata tatttgttta gtttcatttt
     3421 ttctcctata tattcatttt taattttaat tctttaataa tttcgtctac tttaacttta
     3481 gcgttttgaa cagattcacc aacacctata aaataaattt ttagtttagg ttcagttcca
     3541 cttgggcgaa cagcaaatca tgacttatct tctaaataaa attttagtaa gtcttgtcct
     3601 ggcatattat acattccatc gatgtagtct tcaacattaa caactttaag tccagcaatt
     3661 tgagttaagg gtgttgctct caatgatttc attaatggtt caatttttaa tttcttttct
     3721 tctggtttaa aattcaagtt taaagtgaaa gtgtaatatg cacccatttc tttaaataaa
     3781 tcttctaaat agtctactaa tgttttattt tgttttttat aaaatcaagc agcctctgct
     3841 attaatatag aagcttgtat tccatcttta tctctagctg agtcatcaat tacatatcca
     3901 taactttctt cataagcaaa aacaaaattt aatccgttat cttcttcttt agcaatttct
     3961 ctacccattc atttaaatcc agttaaagtt tttacaatat taactccata tttttcatga
     4021 gcgattctat cacccaaatc acttgttaca aaacttgaat atagagccgg attttttgga
     4081 atgctattta agcgttttag atttgataat tttcaatcaa ttaaaattgg tcctgtttga
     4141 tttccatcta atcttacaaa atgaccatca tgttttattg ccattccaaa tctgtcagca
     4201 tctgggtcat tcataataat aatatctgca tcatgtttaa taccatattc aagcggtatt
     4261 tttcatgcag gatcaaattc tggatttgga tttacaacat ttttaaatgt ttcatcttca
     4321 aatgcatgct cttcaacctc aataacgtta tatcctgatt cacgtaatat ttttggggta
     4381 aatttagttc ctgttccatt aactgcgcta aaaataattt ttaaatcttt tttagcttct
     4441 tgctcttttt tgtacgtctc tgttttagag ctagaaatag caagttaaaa taaggctagt
     4501 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttaagcttg gcgtaactag
     4561 atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4621 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4681 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4741 ctttggcgcc ggctcgaggg ggcccgggcg ggtttattac agggacagca gagatccact
     4801 ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     4861 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     4921 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     4981 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     5041 gccggacgtg acaaacggaa gccgcaagtc tcactagtac cctcgcagac ggacagcgcc
     5101 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     5161 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     5221 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     5281 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggc gtacggccac
     5341 catgaaccca gccatcagcg tcgctctcct gctctcagtc ttgcaggtgt cccgagggca
     5401 gaaggtgacc agcctgacag cctgcctggt gaaccaaaac cttcgcctgg actgccgcca
     5461 tgagaataac accaaggata actccatcca gcatgagttc agcctgaccc gagagaagag
     5521 gaagcacgtg ctctcaggca ccctcgggat acccgagcac acgtaccgct cccgcgtcac
     5581 cctctccaac cagccctata tcaaggtcct taccctagcc aacttcacca ccaaggatga
     5641 gggcgactac ttttgtgagc ttcgagtctc gggcgcgaat cccatgagct ccaataaaag
     5701 tatcagtgtg tatagagaca aactggtcaa gtgtggcggc ataagcctgc tggttcagaa
     5761 cacatcctgg atgctgctgc tgctgctttc cctctccctc ctccaagccc tggacttcat
     5821 ttctctgtga acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     5881 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5941 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6001 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6061 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6121 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6181 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6241 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6301 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6361 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6421 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     6481 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6541 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6601 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6661 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6721 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6781 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6841 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6901 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     6961 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7021 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7081 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7141 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7201 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     7261 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     7321 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7381 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7441 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7501 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7561 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7621 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7681 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7741 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7801 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7861 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     7921 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7981 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8041 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8101 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8161 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8221 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8281 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8341 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8401 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8461 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8521 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8581 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8641 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8701 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8761 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8821 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8881 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8941 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9001 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9061 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9121 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9181 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9241 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9301 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9361 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9421 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9481 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9541 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9601 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9661 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9721 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9781 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
//
