LOCUS       sequence-205300-        8668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses V5-tagged miniTurbo on the mammalian ER membrane, 
            lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, 
            Feldman JL, Perrimon N, Ting AY
  TITLE     Efficient proximity labeling in living cells and organisms with 
            TurboID.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201.
  PUBMED    30125270
REFERENCE   2  (bases 1 to 8668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201."
COMMENT     Sequence Label: C1(1-29)-miniTurbo-V5_pLX304
FEATURES             Location/Qualifiers
     source          1..8668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    49..70
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(85..90,91..108,109..115)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    123..139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     128..150
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     147..163
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     147..163
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     182..202
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        184..202
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        230..456
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             457..637
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    684..809
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1939..2439
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2358..2376
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2451..2846
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2906..3023
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3140..3443
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3444..3647
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3598..3618
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3670..3694
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3871..4641
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             4648..4689
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4750..5338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4803..4823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5221..5232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5410..5643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5721..5842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5758..5777)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5812..5831
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5882..6017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6037..6056)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6038..6056)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6066..6083)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6066..6082)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6075..6097)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6224..6679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6311..6330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6521..6542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6705..6809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6810..6878,6879..7670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7841..8429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8330..8349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8583..8600
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
       61 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      121 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
      181 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
      241 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
      301 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
      361 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
      421 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
      481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggaccctt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     1981 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
     2041 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     2101 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     2161 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     2221 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     2281 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     2341 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     2401 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggtaccacc atggccaagc
     2461 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     2521 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     2581 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     2641 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     2701 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     2761 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     2821 tgccctctgg ttatgtgtgg gagggcctgc agctgcagta gtaagaattc tagatcttga
     2881 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2941 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3001 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     3061 gccggctcga gggggcccgg ggttattaat agtaatcaat tacggggtca ttagttcata
     3121 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3181 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3241 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3301 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3361 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3421 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3481 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3541 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3601 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaactg
     3661 tcgggatcaa caagtttgta caaaaaagca ggcttcgaag gagatagaac catggaccct
     3721 gtggtcgtgc tggggctgtg cctgagctgt ctgctgctgc tgagcctgtg gaagcagtcc
     3781 tacggaggcg ggggatcagg aagcggcggc cgcggatcag gaagcggatc aggaagcgga
     3841 tcaggaagcg gatcaggaag cggatcagga atcccgctgc tgaacgctaa acagattctg
     3901 ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac caatcagtac
     3961 ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga atatcagcag
     4021 gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa cctgtacctg
     4081 agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc ggtcatcgga
     4141 attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt caaatggccc
     4201 aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct ggccggaata
     4261 acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat gaggcgcgtg
     4321 gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat taacctggac
     4381 aggaatactc tggccgctat gctgatccga gagctgcggg cagccctgga actgttcgag
     4441 caggaaggcc tggctccata tctgtcacgg tgggagaagc tggataactt catcaataga
     4501 cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg gattgataaa
     4561 cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg cggagaaatc
     4621 agtctgcggt ctgccgaaaa ggctagcggc aagcccatcc ccaaccccct gctgggcctg
     4681 gacagcacct aagacccagc tttcttgtac aaagtgtgct agcgctaacc ggtggcgcgt
     4741 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4801 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4861 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4921 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4981 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5041 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5101 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5161 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5221 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5281 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     5341 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5401 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     5461 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5521 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5581 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5641 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5701 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5761 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5821 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5881 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5941 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6001 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6061 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6121 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6241 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6301 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6361 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6421 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6481 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6541 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6601 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6661 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8581 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8641 cgcgttggcc gattcattaa tgcagctg
//
