LOCUS       sequence-205304-        4229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides N.tabacum RB7 matrix attachment region as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4229)
  TITLE     Moravec lab plasmids
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD1:Rb7-MAR
FEATURES             Location/Qualifiers
     source          1..4229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1394..1412)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1395..1412)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1430..1446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1430..1446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1443..1465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1454..1470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1478..1484,1485..1502,1503..1508))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1523..1544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1661..1678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1832..2420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1912..1931)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2591..3382,3383..3451))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3214..3233
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3452..3556)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3634..4089)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3771..3792)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3983..4002
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(4215..4229,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4229,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tgggcgatga gtggtctcgg
      121 gagtcgatta aaaatcccaa ttatatttgg tctaatttag tttggtattg agtaaaacaa
      181 attcgaacca aaccaaaata taaatatata gtttttatat atatgccttt aagacttttt
      241 atagaatttt ctttaaaaaa tatctagaaa tatttgcgac tcttctggca tgtaatattt
      301 cgttaaatat gaagtgctcc atttttatta actttaaata attggttgta cgatcacttt
      361 cttatcaagt gttactaaaa tgcgtcaatc tctttgttct tccatattca tatgtcaaaa
      421 tctatcaaaa ttcttatata tctttttcga atttgaagtg aaatttcgat aatttaaaat
      481 taaatagaac atatcattat ttaggtatca tattgatttt tatacttaat tactaaattt
      541 ggttaacttt gaaagtgtac atcaacgaaa aattagtcaa acgactaaaa taaataaata
      601 tcatgtgtta ttaagaaaat tctcctataa gaatatttta atagatcata tgtttgtaaa
      661 aaaaattaat ttttactaac acatatattt acttatcaaa aatttgacaa agtaagatta
      721 aaataatatt catctaacaa aaaaaaaacc agaaaatgct gaaaacccgg caaaaccgaa
      781 ccaatccaaa ccgatatagt tggtttggtt tgattttgat ataaaccgaa ccaactcggt
      841 ccatttgcac ccctaatcat aatagcttta atatttcaag atattattaa gttaacgttg
      901 tcaatatcct ggaaattttg caaaatgaat caagcctata tggctgtaat atgaatttaa
      961 aagcagctcg atgtggtggt aatatgtaat ttacttgatt ctaaaaaaat atcccaagta
     1021 ttaataattt ctgctaggaa gaaggttagc tacgatttac agcaaagcca gaatacaaag
     1081 aaccataaag tgattgaagc tcgaaatata cgaaggaaca aatattttta aaaaaatacg
     1141 caatgacttg gaacaaaaga aagtgatata ttttttgttc ttaaacaagc atcccctcta
     1201 aagaatggca gttttccttt gcatgtaact attatgctcc cttcgttaca aaaattttgg
     1261 actactattg ggaacttctt ctgaaaatag tcgctcgaga ccactcatcg cccaatcact
     1321 agtgaattcg cggccgcctg caggtcgacc atatgggaga gctcccaacg cgttggatgc
     1381 atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt
     1441 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1501 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1561 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1621 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1681 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1741 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1801 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1861 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2161 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2221 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2281 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2341 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2401 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2461 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2521 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2581 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2641 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2701 catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct ccagatttat
     2761 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2821 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2881 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2941 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3001 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3061 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3121 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3181 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3241 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3301 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3361 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3421 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3481 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3541 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg tgaaataccg
     3601 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa
     3661 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3721 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3781 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3841 agggcgctgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3901 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3961 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4021 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     4081 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4141 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     4201 aacgccaggg ttttcccagt cacgacgtt
//
