LOCUS       sequence-205308-        4064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides N.tabacum TM2 matrix attachment region as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4064)
  TITLE     Moravec lab plasmids
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD1:TM2-MAR
FEATURES             Location/Qualifiers
     source          1..4064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1229..1247)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1230..1247)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1265..1281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1265..1281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1278..1300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1289..1305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1313..1319,1320..1337,1338..1343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1358..1379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1496..1513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1667..2255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1747..1766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2426..3217,3218..3286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3049..3068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3287..3391)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3469..3924)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3606..3627)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3818..3837
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(4050..4064,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4064,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tgggcgatga gtggtctcgg
      121 gagtcgatta aaaatcccaa ttattgcgag tgaaagctgc tcaatattgc ttaatagaag
      181 aaaatttgta tcgaaatgtt catgggaccc ttagcacggt gccttggtct atcacaaact
      241 gaatatgtga tgcaagaagt acataaagaa cattgttgca atcacgttgg aggaaggtca
      301 ttgttaaaga cgctaaaagg ggcggtatta tttgcaaaaa atagaagaca aagcgaaaat
      361 ttttattaga aggtgtgata aatgtcaacg gggtgcaaac aatatgcatc aaccagcagt
      421 gtttcttcat tcaatcatag caccgtggcc gtttatgaag tgggttatgt attgtaggtc
      481 ctctacctaa agcaccagga aaggaacgac ttttattaat cttaactgat tatttttcta
      541 tgtgggtaga agcatgtgcc tttaaataga tacgagagaa agaggttgtt gatattattt
      601 ggaggaacat aatatgtcga ttttgtgtcc caagaaaaat agtacgcgat aatgggccac
      661 aattcgtagg ttcaaaagtt accgattttc tcaaaatctg gaaaattaaa caaattattt
      721 cggcgcactg ccatcccata gctaatggga aaccataatc gacaaataca attattgtca
      781 acaatataaa gaaacggtta gaatcatcaa aaggcaagtt ccctgaagta ctacttgggc
      841 tagtatggga ttacaggaca acgacgaaga taagcacaag agaaacacca ttctcactta
      901 tttatggtac tgaggcctta atttcggtgg aaataggaga gccaagaaca agatacgtac
      961 atacaaatga agtaacaaat gagaaagaac ttttaacaaa tttagatttg acaaaggaga
     1021 taagggaagc aactttgata cagatggcgg cacaaaagta gaggattgag caatattaca
     1081 acagaaaggc gaaccttcga tatttcaaga ttgggaactt cttcctcgct cgagaccact
     1141 catcgcccaa tcactagtga attcgcggcc gcctgcaggt cgaccatatg ggagagctcc
     1201 caacgcgttg gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat
     1261 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1321 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     1381 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1441 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1501 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac
     2581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg
     3421 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
     3481 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3541 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3601 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3661 gaaaaaccgt ctatcagggc gctggcccac tacgtgaacc atcaccctaa tcaagttttt
     3721 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3781 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3841 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3901 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     3961 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     4021 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgtt
//
