LOCUS       sequence-205310-        3196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3196)
  TITLE     Moravec lab plasmids
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDB1alpha12:empty
FEATURES             Location/Qualifiers
     source          1..3196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      213..648
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    775..797
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     primer_bind     918..937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1131..1153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1145..1162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1146..1162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1163..1219
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1232..1248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1232..1248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1245..1267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1256..1272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1280..1286,1287..1304,1305..1310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1325..1346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1429..1453
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1544..2132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1624..1643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2303..3118)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2303..3118)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3026..3045
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3110..3129
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt
       61 gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt
      121 caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt
      181 ctggctggat gatggggcga ttcaggcgat ccccatccaa cagcccgccg tcgagcgggc
      241 ttttttatcc ccggaagcct gtggatagag ggtagttatc cacgtgaaac cgctaatgcc
      301 ccgcaaagcc ttgattcacg gggctttccg gcccgctcca aaaactatcc acgtgaaatc
      361 gctaatcagg gtacgtgaaa tcgctaatcg gagtacgtga aatcgctaat aaggtcacgt
      421 gaaatcgcta atcaaaaagg cacgtgagaa cgctaatagc cctttcagat caacagcttg
      481 caaacacccc tcgctccggc aagtagttac agcaagtagt atgttcaatt agcttttcaa
      541 ttatgaatat atatatcaat tattggtcgc ccttggcttg tggacaatgc gctacgcgca
      601 ccggctccgc ccgtggacaa ccgcaagcgg ttgcccaccg tcgagcgcct ttgcccacaa
      661 cccggcggcc ggccgcaaca gatcgtttta taaatttttt tttttgaaaa agaaaaagcc
      721 cgaaaggcgg caacctctcg ggcttctgga tttccgatcc ccggaattag atcttggcag
      781 gatatattgt ggtgtaaaga attcgtctca ccatggagag agacccacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      961 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     1021 gctgcgcaac tgttgggaag ggcgatcggc gcaggcctct tcgctattac gccagctggc
     1081 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1141 acgttgtaaa acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga
     1201 cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1261 atccgctcac aattccacac aacatacgag ccggaagcat atagtgtaaa gcctggggtg
     1321 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1381 gaaacctgtc gtgccagctg gggtctctcg ctgctttgag acgaattctg acaggatata
     1441 ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc
     1501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga agaagagttg
     2041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2281 atgtgtaaca ttggtctagt gattagaaaa actcatcgag catcaaatga aactgcaatt
     2341 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     2401 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     2461 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     2521 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     2581 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     2641 aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag
     2701 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     2761 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc cctgggatcg
     2821 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     2881 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     2941 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcggtaga
     3001 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     3061 ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata tggctcataa
     3121 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     3181 tatcttgtgc aatgta
//
