LOCUS       sequence-20533-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GAL-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(29..153)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(161..177)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(161..177)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(192..633)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(198..221)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    516..633
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(657..677)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(657..675)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcctgggg gtggcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta
       61 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      121 cgtttctcgt tcagcttttt tgtacaaact tgtgatgggg gatccactag ttctagaatc
      181 cggggttttt tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac
      241 ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag
      301 tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg
      361 attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa
      421 tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata
      481 accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat
      541 ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg
      601 agggctgtca cccgctcggc ggcttctaat ccgtactaga gctccagctt ttgttccctt
      661 tagtgagggt taattgcgcg cttggcgtaa tcatg
//
