LOCUS       sequence-205350-        2789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP
            and dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-SNCAe3-WT
FEATURES             Location/Qualifiers
     source          1..2789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             103..804
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(883..902)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    948..1003
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1002..1021)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   2418..2452
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
       61 tggttattgt gctgtctcat cattttggca aaggccgcca ccatgagcga gctgattaag
      121 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
      181 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
      241 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
      301 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
      361 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
      421 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
      481 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg
      541 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
      601 gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac
      661 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
      721 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
      781 aaactggggc acaagcttaa ttaacactcc tcaggtgcag gctgcctatc agaaggtggt
      841 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
      901 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
      961 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     1021 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     1081 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     1141 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     1201 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     1261 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     1321 atggagatcc ctcgacctgc agcccaagct tggatcccct aggtttctga tcctgtgtag
     1381 agaccctagt ttgaatgtgc ttatagccta ttattacatg tgtaatgtta cataaattac
     1441 ttaactcgga tttttaattt catcagctat ttaaaatggg cataatataa ctatattaaa
     1501 tggctgttat gaagattaaa taagatgata tgtaaaatgt gttttttgtt tgtttgtttg
     1561 tttgtctgtt tgtttttttg agacagagtc ttgctctgtt acccaggctg gagtgcagtg
     1621 gcacaatctt ggctcactgc aagttctacc tcccgagttc atgccattct cctgcctcag
     1681 cccctcccaa gtagctggga ctacaggcac ccgccaccac gcctggctaa ttttttgtat
     1741 ttttggtaga gatggggttt caccatatta gccaggatgg tctcgatctc ctgacctcgt
     1801 gatctgccca cctcggcctc ccaaattgct gggattacag gcatgagcca ctgcgcccag
     1861 cctaaaatgt ttttttacat aatgggtgtt cagcacatgt taaagccttc tctccatcct
     1921 tcttcccttt tgtttcatgg gttgactgat ctgtctctag tgctgtactt ttaaagcttc
     1981 tacagttctg aattcaaaat tatcttctca ctgggccccg gtgttatctc attctttttt
     2041 ctcctctgta agttgacatg tgatgtggga acaaagggga taaagtcatt attttgtgct
     2101 aaaatcgtaa ttggagagga cctcctgtta gctgggcttt cttctattta ttgtggtggt
     2161 tactggagtt ccttcttcta gttttaggat atatatatat atttttttct ttccctgaag
     2221 atataataat atatatactt ctgaagattg agatttttaa attagttgta ttgaaaacta
     2281 gctaatcagc aatttaaggc tagcttgaga cttatgtctt gaatttgttt ttgtaggctc
     2341 caaaaccaag gagggagtgg tgcatggtgt ggcaacaggt aagctccatt gtgcttatat
     2401 cgaaagatga tatttaaccc tagaaagata gtctgcgtaa aattgacgca tgcattcttg
     2461 aaatattgct ctctctttct aaatagcgcg aatccgtcgc tgtgcattta ggacatctca
     2521 gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc ggtaagtgtc actgattttg
     2581 aactataacg accgcgtgag tcaaaatgac gcatgattat cttttacgtg acttttaaga
     2641 tttaactcat acgataatta tattgttatt tcatgttcta cttacgtgat aacttattat
     2701 atatatattt tcttgttata gatatcaact agaatgctag ttattaatag taatcaatta
     2761 cggggtcatt agttcatagc ccatatatg
//
