LOCUS       sequence-205382-       10212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for creation of PhiC31 attP-flanked platforms for 
            cassette exchange using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Voutev R, Mann RS
  TITLE     Robust PhiC31-Mediated Genome Engineering in Drosophila melanogaster
            Using Minimal attP/attB Phage Sites.
  JOURNAL   G3 (Bethesda). 2018 Mar 9. pii: g3.118.200051. doi: 
            10.1534/g3.118.200051.
  PUBMED    29523637
REFERENCE   2  (bases 1 to 10212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Mar 9. pii: g3.118.200051. doi: 
            10.1534/g3.118.200051."
COMMENT     Sequence Label: pRVV669
FEATURES             Location/Qualifiers
     source          1..10212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            665..4801
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(674..692)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1144..1215,1617..1890,1965..2619,2681..2996,3217..3348,
                     3419..4033)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     CDS             join(6807..6809,6810..6812,6813..7526)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6852..6873)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7113..7132)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7460..7481
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7548..7669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7559..7578)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7613..7632
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(8024..8042)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8063..8079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8063..8079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8076..8098)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8087..8103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8111..8117,8118..8135,8136..8141))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8156..8177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8294..8311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8465..9053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8545..8564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9224..10015,10016..10084))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9847..9866
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10085..10189)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac
      721 attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact
      781 tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc aaaatgacat
      841 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
      901 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc
      961 cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
     1021 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
     1081 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
     1141 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
     1201 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
     1261 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
     1321 aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg
     1381 ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc
     1441 gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
     1501 acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc
     1561 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg
     1621 acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg
     1681 gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc caactagccg
     1741 agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct
     1801 ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac
     1861 cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg
     1921 tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta
     1981 tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa
     2041 tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct
     2101 caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga
     2161 tgacctcttt atcggctccc taacggccag ggaacacctg attttccagg ccatggtgcg
     2221 gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga
     2281 gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct
     2341 gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gcactaaccg atccgccgct
     2401 tctgatctgc gatgagccca cctccggact ggactcattt accgcccaca gcgtcgtcca
     2461 ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc
     2521 ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt
     2581 cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta
     2641 agggtatcta gcattacatt acatctcaac tcctatccag cgtgggtgcc cagtgtccta
     2701 ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga
     2761 tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgctattagc aaagtagccc
     2821 gggatatgga gcagttgttg gccaccaaaa atttggagaa gccactggag cagccggaga
     2881 atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct
     2941 ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga
     3001 gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga
     3061 ttttagttag aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt
     3121 ttatagtttt gcagaaaata aataaatttc atttaactcg cgaacatgtt gaagatatga
     3181 atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt gattggcctc
     3241 atctttttgg gccaacaact cacgcaagtg ggcgtgatga atatcaacgg agccatcttc
     3301 ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt aagtcttgtt
     3361 tagaatacat ttgcatatta ataatttact aactttctaa tgaatcgatt cgatttaggt
     3421 gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt atcgctgtga
     3481 cacatacttt ctgggcaaaa cgattgccga attaccgctt tttctcacag tgccactggt
     3541 cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa
     3601 ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat atctaatatc
     3661 ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta tcataccatt
     3721 cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt
     3781 gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga
     3841 cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa
     3901 ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact acgtgggtct
     3961 ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg
     4021 acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt ttaccattat
     4081 taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca
     4141 ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt
     4201 agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa
     4261 ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg
     4321 cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc
     4381 tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt
     4441 tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg tctcaataga
     4501 aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc
     4561 cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac
     4621 ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt
     4681 gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata tatcaaaaat
     4741 aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc actggaacta
     4801 ggctagattt aaatgcatgc cctgcaggaa gcttcctagg aggcctagat ctgcggccgc
     4861 ccccaactgg ggtaaccttt gagttctctc agttgggggt taattaagaa gttcctattc
     4921 ttcaaaaggt ataggaactt ctctagcccg gcttgctgtt cttcgcgttc aaaatctcca
     4981 gctccatttt gctttcggtg cgcttgcaat cagtactgtc caaaatcgaa aatcgccgaa
     5041 ccgtagtgtg acgtgcgggg ctctgcgaaa ataaactttt ttaggtatat ggccacacac
     5101 ggggaaagca cagtggatta tatgttttaa tattataata tgcaggtttt cattacttat
     5161 ccagatgtaa gcccacttaa agcgatttaa caattatttg ccgaaagagt ataaacaaat
     5221 ttcacttaaa aaatggatta agaaaagctt gtgtaagatt atgcgcagcg ttgccagata
     5281 gctccattta aaacacttca aaaacaataa gttttgaaaa tatatacata aatagcagtc
     5341 gttgccgcaa cgctcaacac atcacacttt taaaacaccc tttacctaca cagaattact
     5401 ttttaaattt ccagtcaagc tgcgagtttc aaaattatag ccggtagaga agacagtgct
     5461 atttcaaaag caaactaaca agggtcttaa attccaaaac accaatccta acaagccttg
     5521 gacttttgta agtttagatc aaaggtggca ttgcattcaa tgtcatggta agaagtaggt
     5581 cgtctaggta gaaatcctca ttcagccggt caagtcagta cgagaaaggt ctcaatttga
     5641 aattgtctta aaaatatttt attgttttgt actgtggtga gtttaaacga aaaacacaaa
     5701 aaaaaagtga tacacagaaa tcataaaaaa ttttaataca aggtattcgt acgtatcaaa
     5761 aacatttcgg cacaattttt tttctctgta ctaaagtgtt acgaacacta cggtattttt
     5821 tagtgatttt caacggacac cgaaggtata taaacagcgt tcgcgaacgg tcgccttcaa
     5881 aaccaattga catttgcagc agcaagtaca agcagaaagt aaagcgcaat cagcgaaaaa
     5941 tttatactta attgttggtg attaaagtac aattaaaaga acattctcga aagtcacaag
     6001 aaacgtaagt ttttaactcg ctgttaccaa ttagtaataa gagcaacaag acgttgagta
     6061 atttcaagaa aaactgcatt tcaaggtctt tgttcggcca tttttttttt attcaacgct
     6121 ctacgtaatt acaaaataag aaattggcag ccacgcatct tgttttccca atcaattggc
     6181 atcaaaacgc aaacaaatct ataaataaaa cttgcgtgtt gattttcgcc aagatttatt
     6241 ggcaaattgt gaaattcgca gtgacgcatt tgaaaattcg agaaatcacg aacgcactcg
     6301 agcatttgtg tgcatgttat tagttagtta gttctttgct taattgaagt attttaccaa
     6361 cgaaatccac ttatttttag ctgaaataga gtaggttgct tgaaacgaaa gccacgtctg
     6421 gaaaatttct tattgcttag tagttgtgac gtcaccatat acacacaaaa taatgtgtat
     6481 gcatgcgttt cagctgtgta tatatacatg cacacactcg cattatgaaa acgatgacga
     6541 gcaacggaac aggtttctca actacctttg ttcctgtttc ttcgctttcc tttgttccaa
     6601 tattcgtaga gggttaatag gggtttctca acaaagttgg cgtcgataaa taagtttccc
     6661 atttttattc cccagccagg aagttagttt caatagtttt gtaatttcaa cgaaactcat
     6721 ttgatttcgt actaattttc cacatctcta ttttctgccc gcagaataat ccaagggcgg
     6781 atcccccggg ctgcaggaat tcaaaaatgg tgagcaaggg cgaggagctg ttcaccgggg
     6841 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     6901 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     6961 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     7021 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     7081 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     7141 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     7201 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     7261 atatcatggc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     7321 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     7381 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     7441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     7501 tcggcatgga cgagctgtac aagtaaggta cctctagaca gacatgataa gatacattga
     7561 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     7621 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     7681 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcgaagt
     7741 tcctattctt caaaaggtat aggaacttca ccggtgacgt ccccccaact gagagaactc
     7801 aaaggttacc ccagttgggg ggcgcgccgc tagccatatg gggattggtg gcgacgactc
     7861 ctggagcccg tcagtatcgg cggaattaca gctgagcgcc ggtcgctacc attaccagtt
     7921 ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag
     7981 gaaatccctc gagggtacct ctagttctag ccagcttttg ttccctttag tgagggttaa
     8041 tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8101 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8161 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8221 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8281 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8341 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8401 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8461 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8521 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8581 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8641 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8701 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8761 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8821 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8881 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8941 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9001 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9061 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9121 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9181 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9241 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9301 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9361 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9421 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9481 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9541 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9601 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9661 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9721 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9781 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9841 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9901 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9961 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10021 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10081 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10141 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10201 gaaaagtgcc ac
//
